4.2.1.30: glycerol dehydratase

This is an abbreviated version!
For detailed information about glycerol dehydratase, go to the full flat file.

Reaction

Synonyms

B12-dependent enzyme glycerol dehydratase, B12-dependent glycerol dehydratase, B12-independent GDH, B12-independent glycerol dehydratase, dehydratase, glycerol, DhaB, DhaB1, DhaBCE, DhaC, DhaE, GDH, GDHt, glycerol dehdydrogenase, glycerol dehydrase, glycerol dehydratase, glycerol hydro-lyase, glycerol hydrolyase, KpG, KpGDHt, vitamin B12-independent glycerol dehydratase

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.30 glycerol dehydratase

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Results	in table
43	Activating Compound
411	AA Sequence
22	Application
1	CAS Registry Number
27	Cloned(Commentary)
63	Cofactor
5	Crystallization (Commentary)
13	General Information
4	General Stability
48	Inhibitors
9	kcat/KM [mM/s]
98	KM Value [mM]
32	Metals/Ions
49	Molecular Weight [Da]
78	Natural Substrates/ Products (Substrates)
176	Organism
2	Oxidation Stability
3	Pathway
12	PDB ID
15	pH Optimum
3	pH Range
4	pH Stability
38	Protein Variants
17	Purification (Commentary)
14	Reaction
1	Reaction Type
117	Reference
27	Specific Activity [micromol/min/mg]
3	Storage Stability
222	Substrates and Products (Substrate)
32	Subunits
66	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
2	Temperature Range [°C]
13	Temperature Stability [°C]
12	Turnover Number [1/s]

KM Value

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KM Value on EC 4.2.1.30 - glycerol dehydratase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.5

(R)-1,2-Propanediol

Klebsiella pneumoniae

Q59476; O08505; Q59475

37°C, pH 7.0, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit

747370

3.07

1,2-ethanediol

Klebsiella pneumoniae

A6NA24, A6NA25, Q59475

pH 8.0, 37°C

713813

0.00002 - 7

1,2-propanediol

34 entries

8 - 20

adenosylcobalamin

2 entries

1.08 - 1.56

ethanediol

2 entries

0.4 - 3.3

glycerol

43 entries

0.7 - 2.6

meso-2,3-butanediol

2 entries

0.2 - 0.24

pentane-1,2-diol

3 entries

0.25 - 0.36

Propane-1,2-diol

3 entries

additional information

additional information

7 entries

-

0.00002

1,2-propanediol

Klebsiella pneumoniae

-

coenzyme B12

33828

0.000021

1,2-propanediol

Klebsiella pneumoniae

-

K+ as cofactor

33828

0.09

1,2-propanediol

Klebsiella pneumoniae

-

mutant I498N, pH 9.0, 42°C

704770

0.12

1,2-propanediol

Klebsiella pneumoniae

-

mutant I498M, pH 8.0, 42°C

704770

0.14

1,2-propanediol

Klebsiella pneumoniae

-

mutant Q42R, pH 9.5, 37°C

704770

0.17

1,2-propanediol

Klebsiella pneumoniae

-

mutant I498V, pH 9.0, 45°C

704770

0.19

1,2-propanediol

Klebsiella pneumoniae

-

mutant I498W, pH 8.0, 42°C. Mutant Q42D, pH 9.5, 42°C, mutant Q42E, pH 8.0, 42°C. Mutant Q42F, pH 9.5, 47°C. Mutant Q42N, pH 9.5, 42°C. Mutant Q42Y, pH 7.5, 42°C

704770

0.2

1,2-propanediol

Klebsiella pneumoniae

-

enzyme in situ, toluene-treated cells

33830

0.24

1,2-propanediol

Klebsiella pneumoniae

A8CIU9

-

673100

0.24

1,2-propanediol

Klebsiella pneumoniae

-

mutant I498C, pH 9.0, 40°C

704770

0.24

1,2-propanediol

Klebsiella pneumoniae

-

mutant Q42M, pH 7.5, 42°C

704770

0.24

1,2-propanediol

Klebsiella pneumoniae

-

wild-type, pH 9.0, 45°C

704770

0.25

1,2-propanediol

Citrobacter freundii

A8CH84

-

673100

0.27

1,2-propanediol

Klebsiella pneumoniae

-

mutant Q42K, pH 9.5, 42°C

704770

0.31

1,2-propanediol

Klebsiella pneumoniae

-

mutant Q42P, pH 9.0, 42°C

704770

0.32

1,2-propanediol

Klebsiella pneumoniae

-

-

675575

0.36

1,2-propanediol

Klebsiella pneumoniae

-

enzyme in vitro

33830

0.37

1,2-propanediol

Klebsiella pneumoniae

-

mutant I498S, pH 7.5, 42°C

704770

0.45

1,2-propanediol

Klebsiella pneumoniae

-

mutant Q42S, pH 9.5, 46.7°C

704770

0.55

1,2-propanediol

Klebsiella pneumoniae

-

mutant Q42H, pH 9.0, 47°C

704770

0.64

1,2-propanediol

Klebsiella pneumoniae

-

mutant Q42I, pH 8.5, 37°C

704770

0.65

1,2-propanediol

Klebsiella pneumoniae

-

mutant Q42G, pH 7.5, 42°C

704770

0.75

1,2-propanediol

Klebsiella pneumoniae

-

mutant Q42A, pH 7.5, 42°C

704770

0.77

1,2-propanediol

Klebsiella pneumoniae

-

mutant Q42L, pH 7.5, 42°C

704770

0.95

1,2-propanediol

Klebsiella pneumoniae

-

mutant I498A, pH 9.0, 45°C

704770

1

1,2-propanediol

Klebsiella pneumoniae

-

mutant I498D, pH 8.0, 45°C

704770

1.13

1,2-propanediol

Klebsiella pneumoniae

-

mutant Q42C, pH 8.0, 42°C

704770

1.2

1,2-propanediol

Klebsiella pneumoniae

-

-

672728

1.38

1,2-propanediol

Klebsiella pneumoniae

-

mutant Q42T, pH 7.5, 42°C

704770

1.43

1,2-propanediol

Klebsiella pneumoniae

A6NA24, A6NA25, Q59475

pH 8.0, 37°C

713813

1.54

1,2-propanediol

Klebsiella pneumoniae

-

mutant Q42V, pH 7.5, 42°C

704770

1.9

1,2-propanediol

Klebsiella pneumoniae

-

mutant I498P, pH 8.0, 42°C

704770

3.3

1,2-propanediol

Klebsiella pneumoniae

-

mutant I498T, pH 8.5, 45°C

704770

7

1,2-propanediol

Limosilactobacillus reuteri

-

in the presence of 0.5 mM coenzyme B12

33833

8

adenosylcobalamin

Klebsiella pneumoniae

-

37°C, recombinant enzyme, purification method 1 and 2

651133

20

adenosylcobalamin

Klebsiella pneumoniae

-

37°C

651133

1.08

ethanediol

Klebsiella pneumoniae

-

enzyme in situ, toluene-treated cells

33830

1.56

ethanediol

Klebsiella pneumoniae

-

enzyme in vitro

33830

0.4

glycerol

Klebsiella pneumoniae

-

mutant Q42L, pH 7.5, 42°C

704770

0.44

glycerol

Klebsiella pneumoniae

-

mutant Q42R, pH 9.5, 37°C

704770

0.48

glycerol

Klebsiella pneumoniae

A6NA24, A6NA25, Q59475

pH 8.0, 37°C

713813

0.48

glycerol

Klebsiella pneumoniae

-

mutant Q42S, pH 9.5, 46.7°C

704770

0.5

glycerol

Klebsiella pneumoniae

-

mutant I498M, pH 8.0, 42°C

704770

0.52

glycerol

Klebsiella pneumoniae

-

mutant I498T, pH 8.5, 45°C

704770

0.53

glycerol

Klebsiella pneumoniae

-

enzyme in situ, toluene-treated cells

33830

0.54

glycerol

Klebsiella pneumoniae

-

enzyme in vitro

33830

0.55

glycerol

Klebsiella pneumoniae

-

mutant I498N, pH 9.0, 42°C

704770

0.57

glycerol

Klebsiella pneumoniae

-

mutant Q42W, pH 7.5, 42°C

704770

0.58

glycerol

Klebsiella pneumoniae

-

mutant I498A, pH 9.0, 45°C

704770

0.59

glycerol

Citrobacter freundii

A8CH84

-

673100

0.59

glycerol

Klebsiella pneumoniae

-

mutant I498V, pH 9.0, 45°C

704770

0.59

glycerol

Citrobacter freundii

A8CH84; A8CH91; A8CH95

at pH 8.5 and 45°C

725951

0.6

glycerol

Klebsiella pneumoniae

A8CIU9

-

673100

0.6

glycerol

Klebsiella pneumoniae

A8CIU9

wild type enzyme, at pH 6.0 and 37°C

729463

0.6

glycerol

Klebsiella pneumoniae

-

wild-type, pH 9.0, 45°C. Mutant I498C, pH 9.0, 40°C. Mutant Q42G, pH 7.5, 42°C. Mutant Q42N, pH 9.5, 42°C

704770

0.6

glycerol

Klebsiella pneumoniae

A8CIU9

mutant enzyme KpG525, at pH 6.0 and 37°C

729463

0.62

glycerol

Klebsiella pneumoniae

-

mutant I498W, pH 8.0, 42°C

704770

0.62

glycerol

Klebsiella pneumoniae

-

mutant Q42E, pH 8.0, 42°C

704770

0.66

glycerol

Klebsiella pneumoniae

-

mutant Q42H, pH 9.0, 47°C

704770

0.67

glycerol

Klebsiella pneumoniae

-

mutant I498S, pH 7.5, 42°C

704770

0.7

glycerol

Klebsiella pneumoniae

-

mutant Q42P, pH 9.0, 42°C

704770

0.7

glycerol

Klebsiella pneumoniae

A8CIU9

mutant enzyme KpG60, at pH 6.0 and 37°C

729463

0.73

glycerol

Klebsiella pneumoniae

Q59476; O08505; Q59475

pH 7.0, 22°C

748257

0.82

glycerol

Klebsiella pneumoniae

-

mutant Q42T, pH 7.5, 42°C

704770

0.9

glycerol

Klebsiella pneumoniae

-

mutant I498P, pH 8.0, 42°C

704770

1.03

glycerol

Klebsiella pneumoniae

-

mutant Q42Y, pH 7.5, 42°C

704770

1.07

glycerol

Klebsiella pneumoniae

-

mutant Q42I, pH 8.5, 37°C

704770

1.1

glycerol

Klebsiella pneumoniae

-

-

675575

1.1

glycerol

Klebsiella pneumoniae

-

37°C, recombinant enzyme, purification method 2

651133

1.18

glycerol

Klebsiella pneumoniae

-

mutant Q42M, pH 7.5, 42°C

704770

1.2

glycerol

Klebsiella pneumoniae

-

37°C, recombinant enzyme, purification method 1

651133

1.22

glycerol

Klebsiella pneumoniae

-

mutant Q42C, pH 8.0, 42°C

704770

1.25

glycerol

Klebsiella pneumoniae

-

in the presence of 0.1 M potassium phosphate buffer

33824

1.3

glycerol

Klebsiella pneumoniae

-

mutant Q42V, pH 7.5, 42°C

704770

1.5

glycerol

Klebsiella pneumoniae

-

37°C

651133

1.5

glycerol

Klebsiella pneumoniae

-

mutant I498D, pH 8.0, 45°C

704770

1.8

glycerol

Klebsiella pneumoniae

-

mutant Q42A, pH 7.5, 42°C

704770

1.94

glycerol

Klebsiella pneumoniae

-

mutant Q42D, pH 9.5, 42°C

704770

2.61

glycerol

Klebsiella pneumoniae

-

mutant Q42F, pH 9.5, 47°C

704770

2.8

glycerol

Klebsiella pneumoniae

-

mutant Q42K, pH 9.5, 42°C

704770

3.3

glycerol

Limosilactobacillus reuteri

-

in the presence of 0.5 mM coenzyme B12

33833

0.7

meso-2,3-butanediol

Klebsiella pneumoniae

Q59476; O08505; Q59475

37°C, pH 7.0, mutant enzyme T200S

747370

2.6

meso-2,3-butanediol

Klebsiella pneumoniae

Q59476; O08505; Q59475

37°C, pH 7.0, linked complex of the fusion protein of alpha- and beta-subunits and the gamma subunit

747370

0.2

pentane-1,2-diol

Klebsiella pneumoniae

A8CIU9

mutant enzyme KpG525, at pH 6.0 and 37°C

729463

0.2

pentane-1,2-diol

Klebsiella pneumoniae

A8CIU9

mutant enzyme KpG60, at pH 6.0 and 37°C

729463

0.24

pentane-1,2-diol

Klebsiella pneumoniae

A8CIU9

wild type enzyme, at pH 6.0 and 37°C

729463

0.25

Propane-1,2-diol

Citrobacter freundii

A8CH84; A8CH91; A8CH95

at pH 8.5 and 45°C

725951

0.32

Propane-1,2-diol

Klebsiella pneumoniae

-

37°C, recombinant enzyme, purification method 1 and 2

651133

0.36

Propane-1,2-diol

Klebsiella pneumoniae

-

37°C

651133

additional information

additional information

Klebsiella pneumoniae

-

-

33823

-

additional information

additional information

Klebsiella pneumoniae

-

-

33833

-

additional information

additional information

Klebsiella pneumoniae

-

-

33835

-

additional information

additional information

Limosilactobacillus reuteri

-

-

33833

-

additional information

additional information

Limosilactobacillus reuteri

-

-

33835

-

additional information

additional information

Klebsiella pneumoniae

-

inactivation kinetics of the enzyme complexes with adenosylcobalamin and its analogs

33827

-

additional information

additional information

Klebsiella pneumoniae

-

adenosylcobalamin analogs

33825

-