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4.2.1.30: glycerol dehydratase

This is an abbreviated version!
For detailed information about glycerol dehydratase, go to the full flat file.

Word Map on EC 4.2.1.30

Reaction

glycerol
=
3-hydroxypropanal
 +
H2O

Synonyms

B12-dependent enzyme glycerol dehydratase, B12-dependent glycerol dehydratase, B12-independent GDH, B12-independent glycerol dehydratase, dehydratase, glycerol, DhaB, DhaB1, DhaBCE, DhaC, DhaE, GDH, GDHt, glycerol dehdydrogenase, glycerol dehydrase, glycerol dehydratase, glycerol hydro-lyase, glycerol hydrolyase, KpG, KpGDHt, vitamin B12-independent glycerol dehydratase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.30 glycerol dehydratase

Temperature Stability

Temperature Stability on EC 4.2.1.30 - glycerol dehydratase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
30 min, potassium phosphate buffer, pH 8.6, apoenzyme is quite stable. On addition of coenzyme B12 to the incubation mixture the holoenzyme is formed
46
-
4 min, 50% destruction of the apoenzyme
50
-
subunit A: quickly inactivated at temperatures above, K+ and Na+ stabilize at high concentrations
60
-
dissociation of the inactive enzyme complex with hydroxocobalamin, if the enzyme complex is heated to over 60°C. Heating products: an active subunit B, an inactive subunit A, and a B12 derivative. K+ inhibit heat-mediated dissociation
75
-
subunit B is stabilized by K+, Na2HPO4 and K2HPO4 protect against thermal inactivation
78
-
K+ stabilizes inactive enzyme complex with hydroxocobalamin
83
-
3 min, 0.6 M KCl, inactive enzyme complex with hydroxocobalamin loses 50% of activity, K+ and Rb+ have the greatest heat-stabilizing effect
additional information