Activating Compound | Comment | Organism | Structure |
---|---|---|---|
coenzyme B12 | GDHt utilizes adenosylcobalamin, i.e. AdoCbl or CoB12, as a cofactor, Km is 10.03 nM | Klebsiella pneumoniae |
Cloned (Comment) | Organism |
---|---|
genes dhaBCE, recombinant expression of His6-tagged GDHt in Escherichia coli strain Bl21(DE3), optimization of IPTG inducting process for recombinant GDHt to increase specific activity and ratio of soluble form using 0.8 mM IPTG at 20°C for 3 h, method development, overview | Klebsiella pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.48 | - |
glycerol | pH 8.0, 37°C | Klebsiella pneumoniae | |
1.43 | - |
1,2-propanediol | pH 8.0, 37°C | Klebsiella pneumoniae | |
3.07 | - |
1,2-ethanediol | pH 8.0, 37°C | Klebsiella pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | A6NA24 | large subunit, gene dhaB; gene dhaB | - |
Klebsiella pneumoniae | A6NA25 | medium subunit, gene dhaC; gene dhaC | - |
Klebsiella pneumoniae | Q59475 | gamma subunit, gene dhaB3 or dhaE; gene dhaB3 or dhaE | - |
Klebsiella pneumoniae XJPD-Li | A6NA24 | large subunit, gene dhaB; gene dhaB | - |
Klebsiella pneumoniae XJPD-Li | A6NA25 | medium subunit, gene dhaC; gene dhaC | - |
Klebsiella pneumoniae XJPD-Li | Q59475 | gamma subunit, gene dhaB3 or dhaE; gene dhaB3 or dhaE | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged GDHt 2.11fold by nickel affinity chromatography and ultrafiltration | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-ethanediol | - |
Klebsiella pneumoniae | acetaldehyde + H2O | - |
? | |
1,2-ethanediol | - |
Klebsiella pneumoniae XJPD-Li | acetaldehyde + H2O | - |
? | |
1,2-propanediol | - |
Klebsiella pneumoniae | propionaldehyde + H2O | - |
? | |
1,2-propanediol | - |
Klebsiella pneumoniae XJPD-Li | propionaldehyde + H2O | - |
? | |
glycerol | - |
Klebsiella pneumoniae | 3-hydroxypropanal + H2O | - |
? | |
glycerol | - |
Klebsiella pneumoniae XJPD-Li | 3-hydroxypropanal + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GDHt | - |
Klebsiella pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant enzyme | Klebsiella pneumoniae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 45 | assay range | Klebsiella pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Klebsiella pneumoniae |
General Information | Comment | Organism |
---|---|---|
metabolism | GDHt is the key enzyme and catalyzes the rate limiting step for biotransformation from glycerol to 1,3-propanediol | Klebsiella pneumoniae |