4.1.99.11: benzylsuccinate synthase

This is an abbreviated version!
For detailed information about benzylsuccinate synthase, go to the full flat file.

(R)-benzylsuccinate synthase, benzylsuccinate synthase, benzylsuccinate synthase A, BSS, BSSA, toluene-activating enzyme

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Results	in table
3	Activating Compound
278	AA Sequence
17	Application
1	CAS Registry Number
12	Cloned(Commentary)
7	Cofactor
1	Crystallization (Commentary)
20	General Information
1	Inhibitors
3	Metals/Ions
14	Molecular Weight [Da]
45	Natural Substrates/ Products (Substrates)
74	Organism
10	Oxidation Stability
6	Pathway
8	PDB ID
5	pH Optimum
1	Posttranslational Modification
12	Protein Variants
7	Purification (Commentary)
12	Reaction
7	Reaction Type
56	Reference
1	Renatured (Commentary)
3	Source Tissue
11	Specific Activity [micromol/min/mg]
87	Substrates and Products (Substrate)
15	Subunits
41	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]
2	Turnover Number [1/s]

Purification on EC 4.1.99.11 - benzylsuccinate synthase

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DEAE-Sepharose, hydroxyapatite, anion exchange chromatography, enzyme severely inactivated during purification

hydroxyapatite, gel filtration, 95% loss of specific activity after gel filtration

loss of activity during purification due to high oxygen sensitivity

recombinant enzyme from Escherichia coli strain BL21(DE3)

using a Ni-NTA-agarose column

using a Strep-Tactin Superflow column