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Literature summary for 4.1.99.11 extracted from
- Biochemical and genetic characterization of benzylsuccinate synthase from Thauera aromatica: a new glycyl radical enzyme catalaysing the first step in anaerobic toluene metabolism (1998), Mol. Microbiol., 28, 615-628.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Thauera aromatica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 10000 | - |
alpha, alpha, beta, gamma, 1 * 94000 + 1 * 90000 + 1 * 12000 + 1 * 10000, SDS-PAGE | Thauera aromatica |
| 12000 | - |
alpha, alpha, beta, gamma, 1 * 94000 + 1 * 90000 + 1 * 12000 + 1 * 10000, SDS-PAGE | Thauera aromatica |
| 90000 | - |
alpha, alpha, beta, gamma, 1 * 94000 + 1 * 90000 + 1 * 12000 + 1 * 10000, SDS-PAGE | Thauera aromatica |
| 94000 | - |
alpha, alpha, beta, gamma, 1 * 94000 + 1 * 90000 + 1 * 12000 + 1 * 10000, SDS-PAGE | Thauera aromatica |
| 220000 | - |
gel filtration | Thauera aromatica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| toluene + fumarate | Thauera aromatica | - |
benzylsuccinate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thauera aromatica | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| rapidly inactivated by oxygen with a half-life of 20-30 s upon exposure to air | Thauera aromatica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose, hydroxyapatite, anion exchange chromatography, enzyme severely inactivated during purification | Thauera aromatica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0002 | - |
- |
Thauera aromatica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| toluene + fumarate | - |
Thauera aromatica | benzylsuccinate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha, alpha', beta, gamma, 1 * 94000 + 1 * 90000 + 1 * 12000 + 1 * 10000, SDS-PAGE | Thauera aromatica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Thauera aromatica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | indicated by fluorescence spectroscopy, chemical nature unknown | Thauera aromatica | |
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Release 2023.1 (January 2023)
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