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4.1.99.11: benzylsuccinate synthase

This is an abbreviated version!
For detailed information about benzylsuccinate synthase, go to the full flat file.

Word Map on EC 4.1.99.11

Reaction

benzylsuccinate
=
Toluene
 +
fumarate

Synonyms

(R)-benzylsuccinate synthase, benzylsuccinate synthase, benzylsuccinate synthase A, BSS, BSSA, toluene-activating enzyme

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.11 benzylsuccinate synthase

Crystallization

Crystallization on EC 4.1.99.11 - benzylsuccinate synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant trimeric enzyme BSS in complex with fumarate and toluene or recombinant dimeric BSSalphagamma enzyme form in complex with fumarate, sitting drop vapor diffusion method, mixing of 8 mg/ml trimeric enzyme in 50 mM Tris, pH 7.6, 15% v/v glycerol, and 200 mM NaCl, in a 2:1 ratio with well solution containing 25% w/v PEG 3350, 100 mM Tris, pH 8.5, 60 mM KCl, and 5 mM fumarate, 0.001-0.002 ml of toluene is added to the bottom of the well and allowed to diffuse slowly into the protein drop, 3 weeks at room temperature. Mixing of 15 mg/ml dimeric enzyme in 20 mM HEPES, pH 7.6, 100 mM NaCl, and 5 mM fumarate, in a 1:1 ratio with well solution containing 20% w/v PEG 400, 50 mM Bis-Tris, pH 6.5, and 25 mM Tris, pH 8.0, several days at room temperature, soaking of crystals in mother liquor with ligands at 50 mM, X-ray diffraction structure determination and analysis at 3.3 A and 2.0 A resolution, respectively, modelling