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4.1.99.11: benzylsuccinate synthase

This is an abbreviated version!
For detailed information about benzylsuccinate synthase, go to the full flat file.

Word Map on EC 4.1.99.11

Reaction

benzylsuccinate
=
Toluene
 +
fumarate

Synonyms

(R)-benzylsuccinate synthase, benzylsuccinate synthase, benzylsuccinate synthase A, BSS, BSSA, toluene-activating enzyme

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.11 benzylsuccinate synthase

Reference

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Reference on EC 4.1.99.11 - benzylsuccinate synthase

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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Krieger, C.J.; Rosebooms, W.; Albrachts, P.J.; Spormann, A.M.
A stable organic free radical in anaerobic benzylsuccinate synthase of Azoarcus sp. strain T
J. Biol. Chem.
276
12924-12927
2001
Azoarcus sp., Azoarcus sp. T
Manually annotated by BRENDA team
Beller, H.R.; Edwards, E.A.
Anaerobic toluene activation by benzylsuccinate synthase in a highly enriched methanogenic culture
Appl. Environ. Microbiol.
66
5503-5505
2000
Desulfotomaculum, Methanothrix sp., Methanospirillum sp.
Manually annotated by BRENDA team
Beller, H.R.; Spormann, A.M.
Substrate range of benzylsuccinate synthase from Azoarcus sp. strain T
FEMS Microbiol. Lett.
178
147-153
1999
Azoarcus sp.
Manually annotated by BRENDA team
Leutwein, C.; Heider, J.
Anaerobic toluene-catabolic pathway in denitrifying Thauera aromatica: activation and beta-oxidation of the first intermediate, (R)-(+)-benzylsuccinate
Microbiology
145
3265-3271
1999
Thauera aromatica
Manually annotated by BRENDA team
Beller, H.R.; Spormann, A.M.
Analysis of the novel benzylsuccinate synthase reaction for anaerobic toluene activation based on structural studies of the product
J. Bacteriol.
180
5454-5457
1998
Azoarcus sp.
Manually annotated by BRENDA team
Leuthner, B.; Leutwein, C.; Schultz,H.; Hörth, P.; Haehnel, W.; Schiltz, E.; Schägger, H.; Heider, J.
Biochemical and genetic characterization of benzylsuccinate synthase from Thauera aromatica: a new glycyl radical enzyme catalaysing the first step in anaerobic toluene metabolism
Mol. Microbiol.
28
615-628
1998
Thauera aromatica
Manually annotated by BRENDA team
Verfürth, K.; Pierik, A.J.; Leutwein, C.; Zorn, S.; Heider, J.
Substrate specificities and electron paramagnetic resonance properties of benzylsuccinate synthases in anaerobic toluene and m-xylene metabolism
Arch. Microbiol.
181
155-162
2004
Azoarcus sp., Azoarcus sp. T, Thauera aromatica, Thauera aromatica K172
Manually annotated by BRENDA team
Qiao, C.; Marsh, E.N.G.
Mechanism of benzylsuccinate synthase: Stereochemistry of toluene addition to fumarate and maleate
J. Am. Chem. Soc.
127
8608-8609
2005
Thauera aromatica
Manually annotated by BRENDA team
Bhandare, R.; Calabro, M.; Coschigano, P.W.
Site-directed mutagenesis of the Thauera aromatica strain T1 tutE tutFDGH gene cluster
Biochem. Biophys. Res. Commun.
346
992-998
2006
Thauera aromatica, Thauera aromatica T1
Manually annotated by BRENDA team
Li, L.; Marsh, E.N.
Deuterium isotope effects in the unusual addition of toluene to fumarate catalyzed by benzylsuccinate synthase
Biochemistry
45
13932-13938
2006
Thauera aromatica
Manually annotated by BRENDA team
Winderl, C.; Schaefer, S.; Lueders, T.
Detection of anaerobic toluene and hydrocarbon degraders in contaminated aquifers using benzylsuccinate synthase (bssA) genes as a functional marker
Environ. Microbiol.
9
1035-1046
2007
Azoarcus sp., Thauera aromatica, Desulfotomaculum sp., Desulfobacula toluolica, Geobacter grbiciae, Geobacter sp., Desulfosarcina cetonica, Desulfotomaculum sp. OX39, Geobacter sp. TMJ1, Azoarcus sp. T, Thauera aromatica K172
Manually annotated by BRENDA team
Li, L.; Patterson, D.P.; Fox, C.C.; Lin, B.; Coschigano, P.W.; Marsh, E.N.
Subunit structure of benzylsuccinate synthase (dagger)
Biochemistry
48
1284-1292
2009
Thauera aromatica
Manually annotated by BRENDA team
Musat, F.; Galushko, A.; Jacob, J.; Widdel, F.; Kube, M.; Reinhardt, R.; Wilkes, H.; Schink, B.; Rabus, R.
Anaerobic degradation of naphthalene and 2-methylnaphthalene by strains of marine sulfate-reducing bacteria
Environ. Microbiol.
11
209-219
2009
uncultured delta proteobacterium
Manually annotated by BRENDA team
Vogt, C.; Cyrus, E.; Herklotz, I.; Schlosser, D.; Bahr, A.; Herrmann, S.; Richnow, H.H.; Fischer, A.
Evaluation of toluene degradation pathways by two-dimensional stable isotope fractionation
Environ. Sci. Technol.
42
7793-7800
2008
Azoarcus sp., Thauera aromatica, Desulfosarcina cetonica, Blastochloris sulfoviridis, Azoarcus sp. T
Manually annotated by BRENDA team
Salinero, K.; Keller, K.; Feil, W.; Feil, H.; Trong, S.; Di Bartolo, G.; Lapidus, A.
Metabolic analysis of the soil microbe Dechloromonas aromatica str. RCB: Indications of a surprisingly complex life-style and cryptic anaerobic pathways for aromatic degradation
BMC Genomics
10
351
2009
no activity in Dechloromonas aromatica RCB
Manually annotated by BRENDA team
Weelink, S.A.; van Doesburg, W.; Saia, F.T.; Rijpstra, W.I.; Roeling, W.F.; Smidt, H.; Stams, A.J.
A strictly anaerobic betaproteobacterium Georgfuchsia toluolica gen. nov., sp. nov. degrades aromatic compounds with Fe(III), Mn(IV) or nitrate as an electron acceptor
FEMS Microbiol. Ecol.
70
575-585
2009
Georgfuchsia toluolica, Georgfuchsia toluolica G5G6
Manually annotated by BRENDA team
Winderl, C.; Penning, H.; Netzer, F.V.; Meckenstock, R.U.; Lueders, T.
DNA-SIP identifies sulfate-reducing Clostridia as important toluene degraders in tar-oil-contaminated aquifer sediment
ISME J.
4
1314-1325
2010
Clostridia
Manually annotated by BRENDA team
Hilberg, M.; Pierik, A.J.; Bill, E.; Friedrich, T.; Lippert, M.L.; Heider, J.
Identification of FeS clusters in the glycyl-radical enzyme benzylsuccinate synthase via EPR and Moessbauer spectroscopy
J. Biol. Inorg. Chem.
17
49-56
2012
Escherichia coli
Manually annotated by BRENDA team
Bharadwaj, V.S.; Dean, A.M.; Maupin, C.M.
Insights into the glycyl radical enzyme active site of benzylsuccinate synthase: a computational study
J. Am. Chem. Soc.
135
12279-12288
2013
Thauera sp. DNT-1 (Q8L1A3)
Manually annotated by BRENDA team
Kuemmel, S.; Kuntze, K.; Vogt, C.; Boll, M.; Heider, J.; Richnow, H.H.
Evidence for benzylsuccinate synthase subtypes obtained by using stable isotope tools
J. Bacteriol.
195
4660-4667
2013
Desulfosarcina cetonica (A2TBP7), Thauera aromatica (O87943), Geobacter metallireducens (Q39VF1), Azoarcus sp. (Q8VPT7), Geobacter metallireducens GS-15 / ATCC 53774 / DSM 7210 (Q39VF1), Desulfosarcina cetonica DSM 7267 (A2TBP7), Azoarcus sp. T (Q8VPT7), Thauera aromatica K172 (O87943)
Manually annotated by BRENDA team
Szaleniec, M.; Heider, J.
Modeling of the reaction mechanism of enzymatic radical C-C coupling by benzylsuccinate synthase
Int. J. Mol. Sci.
17
514
2016
Thauera aromatica (O87943 AND O87944 AND O87942), Thauera aromatica
Manually annotated by BRENDA team
Funk, M.A.; Marsh, E.N.; Drennan, C.L.
Substrate-bound structures of benzylsuccinate synthase reveal how toluene is activated in anaerobic hydrocarbon degradation
J. Biol. Chem.
290
22398-22408
2015
Thauera aromatica (O87943 AND O87944 AND O87942)
Manually annotated by BRENDA team
Heider, J.; Szaleniec, M.; Martins, B.M.; Seyhan, D.; Buckel, W.; Golding, B.T.
Structure and function of benzylsuccinate synthase and related fumarate-adding glycyl radical enzymes
J. Mol. Microbiol. Biotechnol.
26
29-44
2016
Desulfosarcina cetonica, Azoarcus sp. CIB (I6LHT8), Thauera aromatica (O87943 AND O87944 AND O87942), Thauera aromatica K172 (O87943 AND O87944 AND O87942)
Manually annotated by BRENDA team
Funk, M.A.; Judd, E.T.; Marsh, E.N.; Elliott, S.J.; Drennan, C.L.
Structures of benzylsuccinate synthase elucidate roles of accessory subunits in glycyl radical enzyme activation and activity
Proc. Natl. Acad. Sci. USA
111
10161-10166
2014
Thauera aromatica (O87943 AND O87944 AND O87942)
Manually annotated by BRENDA team