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Literature summary for 4.1.99.11 extracted from
- Substrate range of benzylsuccinate synthase from Azoarcus sp. strain T (1999), FEMS Microbiol. Lett., 178, 147-153.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 6500 | - |
alpha, alpha, beta, gamma, 1 * 97000 + 1 * 97000 + 1 * 14500 + 1 * 6500, SDS-PAGE | Azoarcus sp. |
| 14500 | - |
alpha, alpha, beta, gamma, 1 * 97000 + 1 * 97000 + 1 * 14500 + 1 * 6500, SDS-PAGE | Azoarcus sp. |
| 97000 | - |
alpha, alpha, beta, gamma, 1 * 97000 + 1 * 97000 + 1 * 14500 + 1 * 6500, SDS-PAGE | Azoarcus sp. |
| 260000 | - |
gel filtration | Azoarcus sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| toluene + fumarate | Azoarcus sp. | - |
benzylsuccinate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azoarcus sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| hydroxyapatite, gel filtration, 95% loss of specific activity after gel filtration | Azoarcus sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.003 | - |
- |
Azoarcus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-fluorotoluene + fumarate | - |
Azoarcus sp. | (2-fluorobenzyl)succinate | - |
? | |
| 3-fluorotoluene + fumarate | - |
Azoarcus sp. | (3-fluorobenzyl)succinate | - |
? | |
| 4-fluorotoluene + fumarate | - |
Azoarcus sp. | (4-fluorobenzyl)succinate | - |
? | |
| benzaldehyde + fumarate | - |
Azoarcus sp. | benzoylsuccinate | - |
? | |
| m-xylene-d6 + fumarate | - |
Azoarcus sp. | (3-methylbenzyl)succinate-d6 | - |
? | |
| o-xylene-d10 + fumarate | - |
Azoarcus sp. | (2-methylbenzyl)succinate-d10 | - |
? | |
| p-xylene-d10 + fumarate | - |
Azoarcus sp. | (4-methylbenzyl)succinate-d10 | - |
? | |
| toluene + fumarate | - |
Azoarcus sp. | benzylsuccinate | - |
? | |
| toluene + maleate | - |
Azoarcus sp. | benzylsuccinate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha, alpha', beta, gamma, 1 * 97000 + 1 * 97000 + 1 * 14500 + 1 * 6500, SDS-PAGE | Azoarcus sp. |
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