Cloned (Comment) | Organism |
---|---|
recombinant enzyme expression in Escherichia coli strain BL21(DE3) | Thauera aromatica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | one [4Fe4S]-cluster is located in the small subunit beta | Thauera aromatica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
toluene + fumarate | Thauera aromatica | - |
benzylsuccinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thauera aromatica | O87943 AND O87944 AND O87942 | alpha-, beta-, and gamma-subunit | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) | Thauera aromatica |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
benzylsuccinate = toluene + fumarate | benzylsuccinate synthase (BSS) catalyzes the formation of a C-C bond between toluene and fumarate by a radical mechanism, overview. An enzyme-bound glycyl radical cofactor transiently forms a catalytically essential enzyme-bound thiyl (Cys) radical | Thauera aromatica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
toluene + fumarate | - |
Thauera aromatica | benzylsuccinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | 1 * 100000 + 1 * 8500 + 1 * 6500, the enzyme consists of three subunits encoded by three different genes and of very different sizes: the large subunit of circa 100 kDa contains the glycyl radical in the active site and carries out the catalysis | Thauera aromatica |
More | analysis of BSSalpha-BSSgamma subunit interactions and of BSSalpha-BSSbeta subunit interactions, detailed overview | Thauera aromatica |
Synonyms | Comment | Organism |
---|---|---|
BSS | - |
Thauera aromatica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
glycyl radical | an enzyme-bound glycyl radical cofactor transiently forms a catalytically essential enzyme-bound thiyl (Cys) radical | Thauera aromatica | |
additional information | the BSS small subunits resemble high potential iron-sulfur proteins | Thauera aromatica | |
[4Fe-4S] cluster | a [4Fe4S]-cluster is located in the small subunit beta, while there is no bound [4Fe-4S] cluster observable in the electron density of subunit BSSgamma | Thauera aromatica |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme BSS is a member of the glycyl radical enzyme (GRE) family | Thauera aromatica |
additional information | subunit BSSalpha adopts a conserved Gly radical enzyme fold. The beta subunit BSSbeta adopts a fold similar to that of a high potential iron-sulfur protein HiPIP. Subunit gamma, BSSgamma, is only 19% identical to BSSbeta, but they share the basic HiPIP fold along with the extended beta-sheet and hairpin loop. Analysis of BSSalpha-BSSgamma subunit interactions and of BSSalpha-BSSbeta subunit interactions, global structural changes in the BSSalpha-BSSgamma complex, influence of BSSbeta on the structure and flexibility of BSSalpha, overview | Thauera aromatica |
physiological function | benzylsuccinate synthase (BSS) catalyzes the first step in anaerobic toluene degradation, the generation of (R)-benzylsuccinate from toluene and fumarate. This unusual reaction is thought to proceed through radical mediated C-C bond formation, with the radical source being an enzyme-bound glycyl radical cofactor that transiently forms a catalytically essential enzyme-bound thiyl (Cys) radical | Thauera aromatica |