4.1.3.30: Methylisocitrate lyase
This is an abbreviated version!
For detailed information about Methylisocitrate lyase, go to the full flat file.
Word Map on EC 4.1.3.30
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4.1.3.30
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propionate
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methylcitrate
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tuberculosis
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propionyl-coa
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dehydratase
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glyoxylate
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prpc
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lyases
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acetyl-coa
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typhimurium
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mutase
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odd-chain
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3-bromopyruvate
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beta-oxidation
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enterica
- 4.1.3.30
- propionate
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methylcitrate
- tuberculosis
- propionyl-coa
- dehydratase
- glyoxylate
- prpc
- lyases
- acetyl-coa
- typhimurium
- mutase
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odd-chain
- 3-bromopyruvate
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beta-oxidation
- enterica
Reaction
Synonyms
2-Methylisocitrate lyase, Lyase, methylisocitrate, MCL, Mcl1, methylisocitrate lyase, MICL, mmgF, PrpB, TgPrpB, yqiQ
ECTree
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General Information
General Information on EC 4.1.3.30 - Methylisocitrate lyase
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evolution
the 2-methylcitrate cycle enzymes have been acquired via horizontal gene transfer, PrpB phylogenetic analysis, overview
malfunction
metabolism
physiological function
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the DELTAMCL1 strains fail to grow on propionate but exhibit no major changes in other traits, double deletion of both isocitrate lyase 1 and methylisocitrate lyase 1 cause significantly reduced virulence on host plants
malfunction
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a plate confrontation assay does not show a difference between the wild-type and the DELTAmcl deletion strain in antagonism towards Botrytis cinerea. But the DELTAmcl strain displays reduced antagonism towards Botrytis cinerea based on a secretion assay. An in vitro root colonization assay shows that the DELTAmcl strain has reduced ability to colonize Arabidopsis thaliana roots, which results in reduced induction of systemic resistance towards Botrytis cinerea. phenotype, overview. The DELTAmcl strain showed significantly reduced numbers of germinating conidia and delayed conidial pigmentation, as well as decreased tolerance to osmotic stress
malfunction
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absence of MCL activity results in the accumulation of methylisocitrate as a metabolic dead end product arising from the stoichiometric consumption of oxaloacetate by propionyl-CoA, phenotype, overview. Isocitrate lyases are essential for survival on both acetate and propionate because of its methylisocitrate lyase activity. Lack of methylisocitrate lyase activity converts the cell methylcitrate cycle into a dead end pathway that sequesters tricarboxylic acid (TCA) cycle intermediates into methylcitrate cycle intermediates, depletes gluconeogenic precursors, and results in defects of membrane potential and intrabacterial pH
malfunction
an enzyme-disruption mutant prpb-ko shows accumulation of 2-methylcitrate cycle intermediate metabolites, enzyme substrates accumulate in parasites deficient in the enzyme and its absence confers increased sensitivity to propionic acid
malfunction
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the DELTAMCL1 strains fail to grow on propionate but exhibit no major changes in other traits, double deletion of both isocitrate lyase 1 and methylisocitrate lyase 1 cause significantly reduced virulence on host plants
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malfunction
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a plate confrontation assay does not show a difference between the wild-type and the DELTAmcl deletion strain in antagonism towards Botrytis cinerea. But the DELTAmcl strain displays reduced antagonism towards Botrytis cinerea based on a secretion assay. An in vitro root colonization assay shows that the DELTAmcl strain has reduced ability to colonize Arabidopsis thaliana roots, which results in reduced induction of systemic resistance towards Botrytis cinerea. phenotype, overview. The DELTAmcl strain showed significantly reduced numbers of germinating conidia and delayed conidial pigmentation, as well as decreased tolerance to osmotic stress
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metabolism
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role of the methylcitrate cycle in propionate metabolism
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MCL1 is required for the methylcitrate cycle in Gibberella zeae
physiological function
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methylisocitrate lyase is a signature enzyme of the methylcitrate cycle, which is required for propionate metabolism, for secondary metabolite production and for virulence in bacteria and fungi. The enzyme is required in propionate resistance, growth, conidial pigmentation and germination, and abiotic stress tolerance
physiological function
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Mycobacterium tuberculosis isocitrate lyases are catalytically bifunctional isocitrate and methylisocitrate lyases required for growth on even and odd chain fatty acids
physiological function
the enzyme is involved in the 2-methylcitrate cycle required to metabolize and detoxify propionate by oxidation to pyruvate, but the rate-limiting enzyme 2-methylisocitrate lyase is dispensable for parasite survival
physiological function
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the enzyme is essential for the survival of Pseudomonas aeruginosa
physiological function
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MCL1 is required for the methylcitrate cycle in Gibberella zeae
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physiological function
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methylisocitrate lyase is a signature enzyme of the methylcitrate cycle, which is required for propionate metabolism, for secondary metabolite production and for virulence in bacteria and fungi. The enzyme is required in propionate resistance, growth, conidial pigmentation and germination, and abiotic stress tolerance
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