4.1.3.30: Methylisocitrate lyase

This is an abbreviated version!
For detailed information about Methylisocitrate lyase, go to the full flat file.

Reaction

Synonyms

2-Methylisocitrate lyase, Lyase, methylisocitrate, MCL, Mcl1, methylisocitrate lyase, MICL, mmgF, PrpB, TgPrpB, yqiQ

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.3 Oxo-acid-lyases

                4.1.3.30 Methylisocitrate lyase

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Results	in table
3	Activating Compound
11293	AA Sequence
1	CAS Registry Number
7	Cloned(Commentary)
1	Cofactor
3	Crystallization (Commentary)
2	Expression
18	General Information
1	General Stability
28	Inhibitors
4	kcat/KM [mM/s]
1	Ki Value [mM]
12	KM Value [mM]
6	Localization
7	Metals/Ions
7	Molecular Weight [Da]
35	Natural Substrates/ Products (Substrates)
73	Organism
1	Oxidation Stability
6	Pathway
62	PDB ID
3	pH Optimum
3	pH Range
1	pH Stability
1	Posttranslational Modification
13	Protein Variants
5	Purification (Commentary)
3	Reaction
1	Reaction Type
44	Reference
1	Source Tissue
12	Specific Activity [micromol/min/mg]
58	Substrates and Products (Substrate)
4	Subunits
30	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
9	Temperature Stability [°C]
8	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 4.1.3.30 - Methylisocitrate lyase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

19 entries

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

Pyruvate + succinate

15 entries

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate

succinate + pyruvate

5 entries

2-methylisocitrate

succinate + pyruvate

4 entries

2-methylisocitrate lactone

succinate + pyruvate

2 entries

methylisocitrate

succinate + pyruvate

2 entries

additional information

?

-

9 entries

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Aspergillus niger

-

enzyme of propionate catabolism

5348

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Diutina rugosa

-

enzyme of propionate catabolism

5348

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Diutina rugosa IFO 0750

-

enzyme of propionate catabolism

5348

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Mucor rouxianus

-

enzyme of propionate catabolism

5348

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Mucor rouxianus IFO 5773

-

enzyme of propionate catabolism

5348

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Neurospora crassa

-

enzyme of propionate catabolism

5348

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Neurospora crassa IFO 6067

-

enzyme of propionate catabolism

5348

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Rhizopus microsporus var. chinensis

-

enzyme of propionate catabolism

5348

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Rhizopus microsporus var. chinensis IAM 6003

-

enzyme of propionate catabolism

5348

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Ustilago crus-galli

-

enzyme of propionate catabolism

5348

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Ustilago crus-galli F-B-6

-

enzyme of propionate catabolism

5348

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Ustilago utriculosa

-

enzyme of propionate catabolism

5348

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Ustilago utriculosa F-B-5

-

enzyme of propionate catabolism

5348

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Wickerhamiella pararugosa

-

enzyme of propionate catabolism

5348

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Wickerhamiella pararugosa IFO 0966

-

enzyme of propionate catabolism

5348

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Yarrowia lipolytica

-

enzyme synthesis is repressed by glucose

5345

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Yarrowia lipolytica

-

key enzyme in propionate metabolism

5346

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Yarrowia lipolytica

-

the enzyme may function to supply pyruvate, the end-product of the catabolism of the propionate residue, to various reactions

5344

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

?

Yarrowia lipolytica

-

regulatory enzyme of the methylcitric acid cycle

5346

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

Pyruvate + succinate

Aspergillus nidulans

-

syn: methylisocitrate, enzyme acts only on threo-diastereomer, but not on the erythro-diastereomer

651100

-

651100

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

Pyruvate + succinate

Aspergillus nidulans

-

one of the key enzymes of the methylcitrate cycle. Deletion mutants do not grow on propionate as sole carbon and energy source and are severely inhibited during growth on alternative carbon sources, when propionate is present. The strongest inhibitory effect is observed, when glycerol is the main carbon source, followed by glucose and acetate. In addition, asexual conidiation is strongly impaired in the presence of propionate

663709

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

Pyruvate + succinate

Aspergillus nidulans

-

the enzyme is highly specific for (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate

663709

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

Pyruvate + succinate

Escherichia coli

P77541

syn: methylisocitrate

652912

-

652912

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

Pyruvate + succinate

Escherichia coli

P77541

a catalytic mechanism is proposed that involves an R-carboxy-carbanion intermediate/transition state, which is consistent with previous stereochemical experiments showing inversion of configuration at the C(3) of 2-methyl-isocitrate

664091

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

Pyruvate + succinate

Mycobacterium tuberculosis

-

-

681989, 681995

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

Pyruvate + succinate

Mycolicibacterium smegmatis

-

-

681801

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

Pyruvate + succinate

Mycolicibacterium smegmatis mc(2)155 / ATCC 700084

-

-

681801

-

-

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

Pyruvate + succinate

Salmonella enterica

-

syn: methylisocitrate

651720

-

651720

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

Pyruvate + succinate

Yarrowia lipolytica

-

-

5344

-

5344

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

Pyruvate + succinate

Yarrowia lipolytica

-

-

5345

-

5345

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

Pyruvate + succinate

Yarrowia lipolytica

-

reverse reaction not detected

5346

-

5346

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

Pyruvate + succinate

Yarrowia lipolytica

-

specific for threo-DS-2-methylisocitrate

5346

-

5346

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

Pyruvate + succinate

Yarrowia lipolytica

-

no activity with 2R,3S-isocitrate

5346

-

5346

?

(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate

Pyruvate + succinate

Yarrowia lipolytica

-

i.e. 2-methylisocitrate, , threo-DS-2-methylisocitrate

5347

-

5347

?

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate

succinate + pyruvate

Mycobacterium tuberculosis

-

-

728701, 748460, 749369

-

-

?

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate

succinate + pyruvate

Mycobacterium tuberculosis BCG

-

-

749369

-

-

?

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate

succinate + pyruvate

Pseudomonas aeruginosa

-

-

748248

-

-

?

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate

succinate + pyruvate

Trichoderma atroviride

-

-

728261

-

-

?

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate

succinate + pyruvate

Trichoderma atroviride IMI206040

-

-

728261

-

-

?

2-methylisocitrate

succinate + pyruvate

Bacillus subtilis

P54528

-

747131

-

-

?

2-methylisocitrate

succinate + pyruvate

Bacillus subtilis

P54528

the enzyme is involved in the methylcitric acid cycle

747131

-

-

?

2-methylisocitrate

succinate + pyruvate

Bacillus subtilis 168

P54528

-

747131

-

-

?

2-methylisocitrate

succinate + pyruvate

Bacillus subtilis 168

P54528

the enzyme is involved in the methylcitric acid cycle

747131

-

-

?

2-methylisocitrate lactone

succinate + pyruvate

Fusarium graminearum

-

-

703509

-

-

?

2-methylisocitrate lactone

succinate + pyruvate

Fusarium graminearum Z03643

-

-

703509

-

-

?

methylisocitrate

succinate + pyruvate

Aspergillus fumigatus

-

-

714846

-

-

?

methylisocitrate

succinate + pyruvate

Aspergillus fumigatus Af293

-

-

714846

-

-

?

additional information

?

-

Aspergillus nidulans

-

no substrate: isocitrate

651100

-

651100

?

additional information

?

-

Aspergillus nidulans

-

enzyme in methylcitrate cycle

651100

-

651100

?

additional information

?

-

Escherichia coli

P77541

enzyme in methylcitrate cycle

652912

-

652912

?

additional information

?

-

Escherichia coli

-

enzyme in methylcitrate cycle

652912

-

652912

?

additional information

?

-

Mycobacterium tuberculosis

-

Mycobacterium tuberculosis isocitrate lyases are catalytically bifunctional isocitrate and methylisocitrate lyases, EC 4.1.3.1 and EC 4.1.3.30, required for growth on even and odd chain fatty acids

728701

-

-

?

additional information

?

-

Saccharomyces cerevisiae

-

enzyme in methylcitrate cycle

651674

-

651674

?

additional information

?

-

Salmonella enterica

-

no substrate: isocitrate

651720

-

651720

?

additional information

?

-

Salmonella enterica

-

enzyme in methylcitrate cycle

651720

-

651720

?

additional information

?

-

Salmonella enterica subsp. enterica serovar Typhimurium

-

enzyme in methylcitrate cycle

649628

-

649628

?