4.1.3.30: Methylisocitrate lyase

This is an abbreviated version!
For detailed information about Methylisocitrate lyase, go to the full flat file.

Reaction

Synonyms

2-Methylisocitrate lyase, Lyase, methylisocitrate, MCL, Mcl1, methylisocitrate lyase, MICL, mmgF, PrpB, TgPrpB, yqiQ

ECTree

     4 Lyases

         4.1 Carbon-carbon lyases

             4.1.3 Oxo-acid-lyases

                4.1.3.30 Methylisocitrate lyase

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Results	in table
3	Activating Compound
11293	AA Sequence
1	CAS Registry Number
7	Cloned(Commentary)
1	Cofactor
3	Crystallization (Commentary)
2	Expression
18	General Information
1	General Stability
28	Inhibitors
4	kcat/KM [mM/s]
1	Ki Value [mM]
12	KM Value [mM]
6	Localization
7	Metals/Ions
7	Molecular Weight [Da]
35	Natural Substrates/ Products (Substrates)
73	Organism
1	Oxidation Stability
6	Pathway
62	PDB ID
3	pH Optimum
3	pH Range
1	pH Stability
1	Posttranslational Modification
13	Protein Variants
5	Purification (Commentary)
3	Reaction
1	Reaction Type
44	Reference
1	Source Tissue
12	Specific Activity [micromol/min/mg]
58	Substrates and Products (Substrate)
4	Subunits
30	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]
9	Temperature Stability [°C]
8	Turnover Number [1/s]

Specific Activity

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Specific Activity on EC 4.1.3.30 - Methylisocitrate lyase

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.00055

Aspergillus fumigatus

-

substrate isocitrate, S523T mutant protein, pH not specified in the publication, temperature not specified in the publication

714846

0.00105

Aspergillus fumigatus

-

substrate isocitrate, L521F mutant protein, pH not specified in the publication, temperature not specified in the publication

714846

0.0033

Aspergillus fumigatus

-

substrate isocitrate, L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication

714846

0.037

Yarrowia lipolytica

-

-

5345

1.76

Yarrowia lipolytica

-

-

5346

21.4

Aspergillus nidulans

-

-

651100

27.43

Aspergillus fumigatus

-

L521F mutant protein, pH not specified in the publication, temperature not specified in the publication

714846

32.33

Aspergillus fumigatus

-

wild type protein, pH not specified in the publication, temperature not specified in the publication

714846

4.33

Aspergillus fumigatus

-

L521F/S523T mutant protein, pH not specified in the publication, temperature not specified in the publication

714846

7.52

Aspergillus fumigatus

-

S523T mutant protein, pH not specified in the publication, temperature not specified in the publication

714846

91

Aspergillus nidulans

-

-

651100

additional information

Aspergillus fumigatus

-

activity not detectable for substrate isocitrate and wild type protein, pH not specified in the publication, temperature not specified in the publication

714846