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0.91
Met-Ala-Ala
pH 7.0, 97°C, recombinant wild-type
1.8
Met-Pro-Ala
pH 7.0, 97°C, recombinant wild-type
additional information
additional information
-
0.127
Ala-Pro
-
pH 7.8, 37°C, recombinant His-tagged wild-type enzyme
1.495
Ala-Pro
-
pH 7.8, 37°C, recombinant His-tagged mutant F822A
2.219
Ala-Pro
-
pH 7.8, 37°C, recombinant His-tagged mutant V833A
2.397
Ala-Pro
-
pH 7.8, 37°C, recombinant His-tagged mutant Y844A
2.72
Ala-Pro
-
pH 7.8, 37°C, recombinant His-tagged mutant H859A
8.3
Ala-Pro
-
native prolidase
0.006
Gly-L-Pro
-
-
1.64
Gly-L-Pro
-
pH 7.8, 37°C, presence of 20 mM glycine, prolidase I
2.13
Gly-L-Pro
-
in the presence of 0.1 mM colchicine, 1.0 mM Mn2+, in 30 mM phosphate buffer (pH 7.5) 5.0 mM Ru(bpy)32+, at 37°C
2.3
Gly-L-Pro
-
pH 7.8, 37°C, presence of 20 mM D-valine, prolidase I
2.59
Gly-L-Pro
-
pH 7.8, 37°C, prolidase I
2.63
Gly-L-Pro
-
pH 7.8, 37°C, presence of 20 mM L-valine, prolidase I
6.23
Gly-L-Pro
-
in the absence of colchicine, 1.0 mM Mn2+, in 30 mM phosphate buffer (pH 7.5) 5.0 mM Ru(bpy)32+, at 37°C
0.14
Gly-Pro
-
-
2.88 - 2.9
Gly-Pro
-
prolidase I from normal human and mother of patient with prolidase deficiency
0.39
His-Pro
-
-
0.395
L-Leu-L-Pro
-
in the presence of 0.1 mM colchicine, 1.0 mM Mn2+, in 30 mM phosphate buffer (pH 7.5) 5.0 mM Ru(bpy)32+, at 37°C
0.628
L-Leu-L-Pro
-
in the absence of colchicine, 1.0 mM Mn2+, in 30 mM phosphate buffer (pH 7.5) 5.0 mM Ru(bpy)32+, at 37°C
1.7
L-Leu-L-Pro
-
mutant enzyme S307G, in 20 mM sodium citrate (pH 6.5), and 1 mM ZnCl2, at 50°C
8
L-Leu-L-Pro
-
wild type enzyme, in 20 mM sodium citrate (pH 6.5), and 1 mM ZnCl2, at 50°C
10.1
L-Leu-L-Pro
-
mutant enzyme R293S/S307G, in 20 mM sodium citrate (pH 6.5), and 1 mM ZnCl2, at 50°C
14.7
L-Leu-L-Pro
-
mutant enzyme S307R, in 20 mM sodium citrate (pH 6.5), and 1 mM ZnCl2, at 50°C
18
L-Leu-L-Pro
-
mutant enzyme S307D, in 20 mM sodium citrate (pH 6.5), and 1 mM ZnCl2, at 50°C
23.3
L-Leu-L-Pro
-
mutant enzyme R293S, in 20 mM sodium citrate (pH 6.5), and 1 mM ZnCl2, at 50°C
0.81
L-Met-L-Pro
-
-
4.11
L-Met-L-Pro
-
pH 7.8, 37°C, presence of 20 mM glycine, prolidase I
4.43
L-Met-L-Pro
-
pH 7.8, 37°C, presence of 20 mM glycine, enzyme from patient with enzyme deficiency
4.65
L-Met-L-Pro
-
pH 7.8, 37°C, presence of 20 mM D-valine, prolidase I
5.39
L-Met-L-Pro
-
pH 7.8, 37°C, presence of 20 mM glycine, prolidase II
5.48
L-Met-L-Pro
-
pH 7.8, 37°C, presence of 20 mM L-valine, enzyme from patient with enzyme deficiency
5.65
L-Met-L-Pro
-
pH 7.8, 37°C, presence of 20 mM L-valine, prolidase II
6.16
L-Met-L-Pro
-
pH 7.8, 37°C, presence of 20 mM D-valine, enzyme from patient with enzyme deficiency
6.33
L-Met-L-Pro
-
pH 7.8, 37°C, presence of 20 mM D-valine, prolidase II
8.83
L-Met-L-Pro
-
pH 7.8, 37°C, presence of 20 mM L-valine, prolidase I
9.2
L-Met-L-Pro
-
pH 7.8, 37°C, prolidase I
9.65
L-Met-L-Pro
-
pH 7.8, 37°C, prolidase II
9.89
L-Met-L-Pro
-
pH 7.8, 37°C, enzyme from patient with enzyme deficiency
6.8
L-Pro-Gly
-
pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine
8.6
L-Pro-Gly
-
pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine, enzyme from patient with enzyme deficiency
9.7
L-Pro-Gly
-
pH 7.8, 37°C, presence of 0.1 mM MnCl2, enzyme from patient with enzyme deficiency
13.8
L-Pro-Gly
-
pH 7.8, 37°C, presence of 0.1 mM MnCl2
15.1
L-Pro-Gly
-
pH 7.8, 37°C, presence of 20 mM glycine
16.7
L-Pro-Gly
-
pH 7.8, 37°C, presence of 20 mM glycine, enzyme from patient with enzyme deficiency
18.2
L-Pro-Gly
-
pH 7.8, 37°C, enzyme from patient with enzyme deficiency
26.9
L-Pro-Gly
-
pH 7.8, 37°C
4.2
L-Pro-L-Met
-
pH 7.8, 37°C, presence of 20 mM glycine, enzyme from patient with enzyme deficiency
5
L-Pro-L-Met
-
pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine, enzyme from patient with enzyme deficiency
8.1
L-Pro-L-Met
-
pH 7.8, 37°C, enzyme from patient with enzyme deficiency
8.35
L-Pro-L-Met
-
pH 7.8, 37°C, presence of 20 mM glycine
9.7
L-Pro-L-Met
-
pH 7.8, 37°C, presence of 0.1 mM MnCl2, enzyme from patient with enzyme deficiency
9.9
L-Pro-L-Met
-
pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine
17.6
L-Pro-L-Met
-
pH 7.8, 37°C
19.1
L-Pro-L-Met
-
pH 7.8, 37°C, presence of 0.1 mM MnCl2
3.2
L-Pro-L-Val
-
pH 7.8, 37°C, presence of 20 mM glycine, enzyme from patient with enzyme deficiency
5.5
L-Pro-L-Val
-
pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine, enzyme from patient with enzyme deficiency
7.9
L-Pro-L-Val
-
pH 7.8, 37°C, enzyme from patient with enzyme deficiency
10.1
L-Pro-L-Val
-
pH 7.8, 37°C, presence of 0.1 mM MnCl2, enzyme from patient with enzyme deficiency
14.7
L-Pro-L-Val
-
pH 7.8, 37°C, presence of 20 mM glycine
17.3
L-Pro-L-Val
-
pH 7.8, 37°C, presence of 0.1 mM MnCl2 plus 20 mM glycine
26
L-Pro-L-Val
-
pH 7.8, 37°C
28.6
L-Pro-L-Val
-
pH 7.8, 37°C, presence of 0.1 mM MnCl2
0.06
L-Val-L-Pro
-
-
0.243
L-Val-L-Pro
-
in the presence of 0.1 mM colchicine, 1.0 mM Mn2+, in 30 mM phosphate buffer (pH 7.5) 5.0 mM Ru(bpy)32+, at 37°C
0.269
L-Val-L-Pro
-
in the absence of colchicine, 1.0 mM Mn2+, in 30 mM phosphate buffer (pH 7.5) 5.0 mM Ru(bpy)32+, at 37°C
0.2
Leu-Pro
-
-
0.81
Leu-Pro
pH 7.0, 70°C, mutant enzyme A195T/G306S
0.92
Leu-Pro
pH 7.0, 70°C, wild-type enzyme
0.98
Leu-Pro
pH 7.0, 70°C, mutant enzyme E127G/E252D
1.6
Leu-Pro
pH 7.0, 70°C, mutant enzyme E36V
2.92
Leu-Pro
pH 7.0, 70°C, mutant enzyme Y301C/K342N
3
Leu-Pro
-
native prolidase
10.4
Leu-Pro
-
human fibroplasts with prolidase deficiency
1.5
Lys-Pro
-
pH 8.0, 55°C
0.81
Met-Pro
-
-
1.7
Met-Pro
-
pH 8.0, 55°C
1.9
Met-Pro
pH 7.0, 100°C
2.8
Met-Pro
-
native prolidase
3.3
Met-Pro
-
recombinant prolidase
3.4
Met-Pro
pH 7.0, 100°C
5.7
Met-Pro
pH 7.0, 70°C, recombinant wild-type enzyme
5.7
Met-Pro
pH 7.0, 70°C, wild-type enzyme
6.4
Met-Pro
pH 7.0, 35°C, wild-type enzyme
6.4
Met-Pro
pH 7.0, 35°C, recombinant wild-type enzyme
6.8
Met-Pro
pH 7.0, 70°C, recombinant mutant G39E
6.8
Met-Pro
pH 7.0, 70°C, wild-type enzyme G39E
7.5
Met-Pro
pH 7.0, 70°C, recombinant mutant R19G/G39E/K71E/S229T
7.5
Met-Pro
pH 7.0, 70°C, mutant enzyme R19G/G39E/K71E/S229T
7.9
Met-Pro
pH 7.0, 35°C, recombinant mutant R19G/G39E/K71E/S229T
7.9
Met-Pro
pH 7.0, 35°C, mutant enzyme R19G/G39E/K71E/S229T
7.95
Met-Pro
pH 7.0, 100°C, recombinant wild-type enzyme
7.95
Met-Pro
pH 7.0, 100°C, wild-type enzyme
9.2
Met-Pro
pH 7.0, 70°C, recombinant mutant R19G/K71E/S229T
9.2
Met-Pro
pH 7.0, 70°C, mutant enzyme R19G/K71E/S229T
9.8
Met-Pro
pH 7.0, 97°C, recombinant wild-type
11.1
Met-Pro
pH 7.0, 100°C, recombinant mutant R19G/G39E/K71E/S229T
11.1
Met-Pro
pH 7.0, 100°C, mutant enzyme R19G/G39E/K71E/S229T
11.3
Met-Pro
pH 7.0, 35°C, recombinant mutant R19G/K71E/S229T
11.3
Met-Pro
pH 7.0, 35°C, mutant enzyme R19G/K71E/S229T
13
Met-Pro
pH 7.0, 35°C, recombinant mutant G39E
13
Met-Pro
pH 7.0, 35°C, mutant enzyme G39E
13.7
Met-Pro
pH 7.0, 100°C, recombinant mutant R19G/K71E/S229T
13.7
Met-Pro
pH 7.0, 100°C, mutant enzyme R19G/K71E/S229T
14.5
Met-Pro
pH 7.0, 100°C, recombinant mutant G39E
14.5
Met-Pro
pH 7.0, 100°C, wild-type enzyme G39E
0.76
Phe-Pro
-
-
1.3
Phe-Pro
-
pH 8.0, 55°C
20
Phe-Pro
-
native prolidase
0.38
Pro-Pro
-
-
0.41
Val-Pro
-
-
4.2
Val-Pro
-
native prolidase
additional information
additional information
-
-
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics of wild-type and mutant enzymes
-
additional information
additional information
-
kinetics and substrate specificity of wild-type and mutant enzymes, overview
-
additional information
additional information
the recombinant enzyme shows an allosteric behaviour, overview
-