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Literature summary for 3.4.13.9 extracted from
- Isolation and characterization of a prolidase from Streptococcus cremoris H61 (1984), Agric. Biol. Chem., 48, 3035-3040.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Lactococcus cremoris |  |
| 2-mercaptoethanol | - |
Lactococcus cremoris | |
| Cu2+ | - |
Lactococcus cremoris | |
| EDTA | - |
Lactococcus cremoris | |
| Hg2+ | - |
Lactococcus cremoris | |
| N-ethylmaleimide | - |
Lactococcus cremoris | |
| p-chloromercuribenzoate | - |
Lactococcus cremoris | |
| Zn2+ | slightly | Lactococcus cremoris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.41 | - |
Val-Pro | - |
Lactococcus cremoris | |
| 0.61 | - |
Ala-Pro | - |
Lactococcus cremoris | |
| 0.76 | - |
Phe-Pro | - |
Lactococcus cremoris | |
| 0.82 | - |
Leu-Pro | - |
Lactococcus cremoris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
gel filtration | Lactococcus cremoris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactococcus cremoris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lactococcus cremoris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Lactococcus cremoris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Ala-Pro + H2O | - |
Lactococcus cremoris | L-Ala + Pro | - |
? | |
| L-Phe-Pro + H2O | - |
Lactococcus cremoris | L-Phe + Pro | - |
? | |
| L-Val-Pro + H2O | - |
Lactococcus cremoris | L-Val + Pro | - |
? | |
| Leu-Pro + H2O | - |
Lactococcus cremoris | Leu + Pro | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Lactococcus cremoris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
inactivation above | Lactococcus cremoris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Lactococcus cremoris |
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