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1.6.2.2: cytochrome-b5 reductase

This is an abbreviated version!
For detailed information about cytochrome-b5 reductase, go to the full flat file.

Word Map on EC 1.6.2.2

Reaction

NADH
+ 2 ferricytochrome b5 =
NAD+
 +
H+
 + 2 ferrocytochrome b5

Synonyms

b5 plusb5R, b5/b5R, B5R, cb5r, CBR, CBR1, CBR1A, CBR1B, CyB5R, CYB5R2, CYB5R3, Cyb5R4, cyt b5r, cyt b5R protein, cytochrome b5 reductase, cytochrome b5 reductase 2, cytochrome b5 reductase 3, DIA1, diaphorase I, dihydronicotinamide adenine dinucleotide-cytochrome b5 reductase, HPO-19, methemoglobin reductase, NADH cytochrome b5 oxidoreductase, NADH cytochrome B5 reductase, NADH-b5R, NADH-cytochrome b 5 reductase, NADH-cytochrome b5 reductase, NADH-cytochrome b5 reductase 3, NADH-cytochrome-b5 reductase, NADH-cytochrome-b5 reductase 3, NADH-dependent cytochrome b5 reductase, NADH-ferricyanide reductase, NADH-ferricytochrome b5 oxidoreductase, NADH-ferricytochrome reductase, NADH: ferricytochrome b5 oxidoreductase, NADH:cytochrome b5 reductase, NADH:ferricytochrome b5 oxidoreductase, Ncb5or, P34/P32, P35, reduced nicotinamide adeninedinucleotide-cytochrome b5 reductase, reductase, cytochrome b5, T05H4.4

ECTree

     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.2 With a heme protein as acceptor
                1.6.2.2 cytochrome-b5 reductase

Specific Activity

Specific Activity on EC 1.6.2.2 - cytochrome-b5 reductase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00072
-
liver microsomes, reduction of 1,2-dihydro-8-(4-methylpiperazin-1-yl)-4-phenylimidazol[3,2-e]pyrazine 5-oxide
0.0039
-
activity in liver microsomes
0.036 - 0.109
-
activity in lysates of different cancer cell lines
0.0648
-
activity in liver microsomes in the presence of 2 mM MgCl2
0.0888
-
activity in liver microsomes in the presence of 2 mM CaCl2
0.109
-
reduction of aquacobalamin
0.372
-
in the presence of 0.1% Triton X-100
1.69
-
methemoglobin-ferrocyanide reductases assay
10.6
-
low MW aggregate, cytochrome b as electron acceptor
1007
-
reduction of ferricyanide
1200 - 1300
-
ferricyanide reduction
137
-
-
138
-
low MW aggregate, dichlorophenolindophenol as electron acceptor
14.9
-
pH 7.0, 25°C, substrate ferricyanide
1400
-
recombinant enzyme
1470
-
enzyme from liver plasma membrane
1510
-
liver microsomal enzyme, ferricyanide as electron acceptor
2.042
-
reduction of cytochrome b5
230.5
-
high MW aggregate
2480
-
reduction of ferricyanide
3.5
-
low MW aggregate, nitro blue tetrazolium as electron acceptor
611.1
-
low MW aggregate, ferricyanide as electron acceptor
670
-
-
96.3
-
recombinant enzyme
additional information