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1.6.2.2: cytochrome-b5 reductase

This is an abbreviated version!
For detailed information about cytochrome-b5 reductase, go to the full flat file.

Word Map on EC 1.6.2.2

Reaction

NADH
+ 2 ferricytochrome b5 =
NAD+
 +
H+
 + 2 ferrocytochrome b5

Synonyms

b5 plusb5R, b5/b5R, B5R, cb5r, CBR, CBR1, CBR1A, CBR1B, CyB5R, CYB5R2, CYB5R3, Cyb5R4, cyt b5r, cyt b5R protein, cytochrome b5 reductase, cytochrome b5 reductase 2, cytochrome b5 reductase 3, DIA1, diaphorase I, dihydronicotinamide adenine dinucleotide-cytochrome b5 reductase, HPO-19, methemoglobin reductase, NADH cytochrome b5 oxidoreductase, NADH cytochrome B5 reductase, NADH-b5R, NADH-cytochrome b 5 reductase, NADH-cytochrome b5 reductase, NADH-cytochrome b5 reductase 3, NADH-cytochrome-b5 reductase, NADH-cytochrome-b5 reductase 3, NADH-dependent cytochrome b5 reductase, NADH-ferricyanide reductase, NADH-ferricytochrome b5 oxidoreductase, NADH-ferricytochrome reductase, NADH: ferricytochrome b5 oxidoreductase, NADH:cytochrome b5 reductase, NADH:ferricytochrome b5 oxidoreductase, Ncb5or, P34/P32, P35, reduced nicotinamide adeninedinucleotide-cytochrome b5 reductase, reductase, cytochrome b5, T05H4.4

ECTree

     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.2 With a heme protein as acceptor
                1.6.2.2 cytochrome-b5 reductase

Purification

Purification on EC 1.6.2.2 - cytochrome-b5 reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography on ADP-agarose
-
ammonium sulfate precipitation, DE-52 cellulose column chromatography, hydroxylapatite column chromatography, and NAD+-agarose column chromatography
-
ammonium sulfate precipitation, DEAE-Sepharose CL-6B column chromatography, 5'-ADP-agarose (or 5'-AMP-agarose) gel column chromatography, and Ni-NTA-agarose column chromatography
-
chimeric protein NADH-cytochrome b5 reductase-cytochrome b5
-
DEAE-cellulose column chromatography, DEAE-Sepharose column chromatography, DEAE-Trisacryl column chromatography, agarose-hexane-NAD column chromatography, ADP-agarose column chromatography, 5'-AMP-Sepharose column chromatography, blue-Ultrogel column chromatography, octylamino-Sepharose column chromatography, blue 2-Sepharose column chromatography, Sephadex G-100 gel filtration, 5'-ADP-hexane-agarose column chromatography, hydroxyapatite column chromatography, CM-Sephadex gel filtration, or NAD agarose column chromatography
DEAE-cellulose column chromatography, Toyopearl butyl column chromatography, and Sephacryl S200 gel filtration
-
DEAE-cellulose, 5'-ADP-agarose affinity chromatography
-
DEAE-cellulose, DEAE-Sephadex, hydroxyapatite
-
DEAE-trisacryl, Blue ultrogel, Mono Q
lysosome- and detergent-solubilization
-
lysosome-solubilization
native enzyme from liver
-
native enzyme from liver by anion-exchange chromatography of the detergent solubilized microsomes on two successive DEAE-cellulose columns and an affinity chromatography on adenosine 5'-diphosphate-agarose, to homogeneity
-
native enzyme from liver microsomes by anion exchange chromatography and affinity chromatography
-
native membrane-bound isozyme by solubiization from membranes, anion exchange chromatography, and affinity chromatography
-
Ni-NTA column chromatography
Ni2+-NTA bead chromatography, HiTrap Q column chromatography, HiTrap phenyl column chromatography, and Superdex 200 gel filtration
polyethylene glycol precipitation, ADP-agarose
-
polyethylene glycol precipitation, DEAE-cellulose, hydroxylapatite, ADP-agarose
-
recombinant enzyme
recombinant enzyme, affinity chromatography on 5'-ADP-agarose
-
recombinant enzymes
recombinant enzymes from Escherichia coli
recombinant enzymes purified from Escherichia coli
-
recombinant protein
-
recombinant wild-type, H49A, H49E, H49K, H49Y, and DELTAF272 mutant enzyme
-
recombinant wild-type, K110A, K110M and K110R mutant enzyme
-
recombinant wild-type, K41A, K125A and K163A mutant enzyme
-
recombinant wild-type, K97A, K97R, S99A, S99T, S99V, R63A, R63Q, R63K, Y65A and Y65F mutant enzymes
-
separation of membrane binding and catalytic domain
-
soluble domain
-
Triton X-100, anion exchange chromatography, gel filtration
-
Triton X-100, DEAE-cellulose, CM-cellulose, 5'-ADP-agarose
-
wild-type, K110R, K110H, K110A, K110E and K110Q mutant enzymes