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Literature summary for 1.6.2.2 extracted from
- Purification and properties of soluble NADH-cytochrome b5 reductase of rabbit erythrocytes (1982), J. Biochem., 91, 1467-1477.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Atebrin | 0.5 mM, complete inhibition | Oryctolagus cuniculus |  |
| iodoacetamide | 1 mM, complete inhibition | Oryctolagus cuniculus | |
| N-ethylmaleimide | 1 mM, 90% inhibition | Oryctolagus cuniculus | |
| p-chloromercuribenzoate | 0.001 mM, complete inhibition | Oryctolagus cuniculus | |
| Proflavin | 0.1 mM, 74% inhibition | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0025 | - |
NADH | - |
Oryctolagus cuniculus | |
| 0.009 | - |
ferricytochrome b5 | - |
Oryctolagus cuniculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | rate of reduction depends on ionic strength | Oryctolagus cuniculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
gel filtration | Oryctolagus cuniculus |
| 34700 | - |
erythrocytes, cytosol, gel filtration, calculation from FAD content | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5 | ordered bi bi mechanism | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | soluble form | Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 497 | - |
- |
Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 25 mM phosphate buffer, pH 7.6, 1 mM EDTA, 0.1 mM dithiothreitol, at least a few months | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricytochrome b5 + NADH | specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol | Oryctolagus cuniculus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| 2 ferricytochrome b5 + NADH | additional acceptor: hemin | Oryctolagus cuniculus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| 2 ferricytochrome b5 + NADH | poor donor: NADPH | Oryctolagus cuniculus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
| NADH + ferricytochrome b5 | - |
Oryctolagus cuniculus | NAD+ + H+ + ferrocytochrome b5 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 33000, soluble enzyme from erythrocyte, SDS-PAGE | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
no distinct optimum with phosphate or Tris-HCl buffer | Oryctolagus cuniculus |
| 6.4 | - |
citrate-phosphate buffer | Oryctolagus cuniculus |
| 6.6 | - |
Tris-maleate buffer | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 1 mol FAD per mol enzyme | Oryctolagus cuniculus | |
| NADH | - |
Oryctolagus cuniculus | |
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