Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
NADH | - |
Homo sapiens | |
0.00114 | - |
cytochrome b5 | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
recombinant enzyme, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome b5 + NADH | Homo sapiens | soluble form of erythrocytes: reduction of methemoglobin | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
lipoprotein | myristic acid at NH2-terminus, amide bond, no ester bond | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | soluble form | Homo sapiens | - |
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
96.3 | - |
recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome b5 + NADH | soluble form of erythrocytes: reduction of methemoglobin | Homo sapiens | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
NADH + ferricytochrome b5 | - |
Homo sapiens | NAD+ + H+ + ferrocytochrome b5 | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol FAD per mol of recombinant enzyme | Homo sapiens | |
NADH | - |
Homo sapiens |