Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
NADH | recombinant enzyme | Rattus norvegicus | |
0.007 | - |
ferricyanide | recombinant enzyme | Rattus norvegicus | |
0.011 | - |
ferricytochrome b5 | recombinant enzyme | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
1 * 30000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
30588 | - |
1 * 30588, recombinant enzyme, MALDI-TOF mass spectrometry | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, affinity chromatography on 5'-ADP-agarose | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1400 | - |
recombinant enzyme | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ferricytochrome b5 + NADH | recombinant enzyme, very low activity with NADPH | Rattus norvegicus | 2 ferrocytochrome b5 + NAD+ + H+ | - |
? | |
NADH + ferricytochrome b5 | - |
Rattus norvegicus | NAD+ + H+ + ferrocytochrome b5 | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
monomer | 1 * 30588, recombinant enzyme, MALDI-TOF mass spectrometry | Rattus norvegicus |