General Stability | Organism |
---|---|
dithiothreitol is essential for stability | Phascolopsis gouldii |
EDTA is essential for stability | Phascolopsis gouldii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by NAD+ and ferrocyanide | Phascolopsis gouldii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
cytochrome b5 | - |
Phascolopsis gouldii | |
0.06 | - |
NADH | - |
Phascolopsis gouldii | |
0.09 | - |
ferricyanide | - |
Phascolopsis gouldii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Phascolopsis gouldii | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE | Phascolopsis gouldii |
200000 | - |
oligomeric aggregate of detergent-solubilized enzyme in aqueous media, gel filtration | Phascolopsis gouldii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phascolopsis gouldii | - |
sipuncilid worm | - |
Oxidation Stability | Organism |
---|---|
O2, solubilized enzyme very sensitive to atmospheric oxygen | Phascolopsis gouldii |
Purification (Comment) | Organism |
---|---|
- |
Phascolopsis gouldii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | soluble form | Phascolopsis gouldii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.5 | - |
low MW aggregate, nitro blue tetrazolium as electron acceptor | Phascolopsis gouldii |
10.6 | - |
low MW aggregate, cytochrome b as electron acceptor | Phascolopsis gouldii |
138 | - |
low MW aggregate, dichlorophenolindophenol as electron acceptor | Phascolopsis gouldii |
230.5 | - |
high MW aggregate | Phascolopsis gouldii |
611.1 | - |
low MW aggregate, ferricyanide as electron acceptor | Phascolopsis gouldii |
Storage Stability | Organism |
---|---|
liquid N2 | Phascolopsis gouldii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methemerythrin + NADH | - |
Phascolopsis gouldii | deoxymethemerythrin + NAD+ | - |
? | |
NADH + ferricyanide | - |
Phascolopsis gouldii | NAD+ + H+ + ferrocyanide | - |
r | |
NADH + ferricytochrome b5 | - |
Phascolopsis gouldii | NAD+ + H+ + ferrocytochrome b5 | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Phascolopsis gouldii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
acceptor ferricyanide | Phascolopsis gouldii |
6 | 8 | acceptor Phascolopsis gouldii cytochrome b5 | Phascolopsis gouldii |
8 | - |
acceptor dichlorphenolindophenol | Phascolopsis gouldii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Phascolopsis gouldii | |
NADH | - |
Phascolopsis gouldii |