1.2.1.87: propanal dehydrogenase (CoA-propanoylating)

This is an abbreviated version!
For detailed information about propanal dehydrogenase (CoA-propanoylating), go to the full flat file.

Reaction

propanal
+
CoA
+
NAD+
=
propanoyl-CoA
+
NADH
+
H+

Synonyms

BphJ, CoA-acylating aldehyde dehydrogenase, coenzyme A-acylating aldehyde dehydrogenase, More, PduP, TTHB247

ECTree

     1 Oxidoreductases
         1.2 Acting on the aldehyde or oxo group of donors
             1.2.1 With NAD+ or NADP+ as acceptor
                1.2.1.87 propanal dehydrogenase (CoA-propanoylating)

Purification

Purification on EC 1.2.1.87 - propanal dehydrogenase (CoA-propanoylating)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
BphI and BphJ form a stable complex as they bind and coelute from Ni2+-NTA column, after purification N-terminal histidine tag of BphJ is proteolytically cleaved by thrombin digestion
-
BphI and BphJ form a stable complex as they bind and coelute from Ni2+-NTA column, although only BphJ has the histidine tag. After purification, the N-terminal histidine tag of BphJ is proteolytically cleaved by thrombin digestion
purified to homogeneity using Ni2+-NTA chromatography