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Literature summary for 1.2.1.87 extracted from

  • Baker, P.; Hillis, C.; Carere, J.; Seah, S.Y.K.
    Protein-protein interactions and substrate channeling in orthologous and chimeric aldolase-dehydrogenase complexes (2012), Biochemistry, 51, 1942-1952.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
chimeric complexes of Burkholderia xenovorans and Thermus thermophilus enzymes, TTHB246-BphJ and BphI-TTHB247 created by coexpression of the relevant genes in Escherichia coli using separate expression plasmids Thermus thermophilus
separate expression of TTHB247 in recombinant Escherichia coli Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5
-
acetaldehyde app. Km-value, single TTHB247, pH 8.0, 25°C Thermus thermophilus
6.4
-
propionaldehyde app. Km-value, single TTHB247, pH 8.0, 25°C Thermus thermophilus
9.4
-
acetaldehyde app. Km-value, TTHB247-BphI complex, pH 8.0, 25°C Thermus thermophilus
9.6
-
propionaldehyde app. Km-value, TTHB247-BphI complex, pH 8.0, 25°C Thermus thermophilus
15.4
-
acetaldehyde app. Km-value, TTHB246-TTHB247 complex, pH 8.0, 25°C Thermus thermophilus
15.7
-
acetaldehyde app. Km-value, BphJ-TTHB246 complex, pH 8.0, 25°C Thermus thermophilus
16.1
-
propionaldehyde app. Km-value, BphJ-TTHB246 complex, pH 8.0, 25°C Thermus thermophilus
16.1
-
propionaldehyde app. Km-value, TTHB246-TTHB247 complex, pH 8.0, 25°C Thermus thermophilus
23.1
-
propionaldehyde app. Km-value, BphJ-BphI complex, pH 8.0, 25°C Thermus thermophilus
23.6
-
acetaldehyde app. Km-value, BphJ-BphI complex, pH 8.0, 25°C Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
estimated by SDS-PAGE, in agreement with predicted molecular mass calculated from amino acid sequences Thermus thermophilus
33000
-
native molecular mass of TTHB247, determined by static light scattering Thermus thermophilus
40000
-
native molecular mass of TTHB247, determined by gel filtration Thermus thermophilus
137000
-
molecular mass of BphI-TTHB247 chimeric complex, determined by static light scattering Thermus thermophilus
142000
-
native molecular mass of TTHB246-TTHB247 complex, determined by gel filtration Thermus thermophilus
144000
-
molecular mass of BphI-TTHB247 chimeric complex, determined by gel filtration Thermus thermophilus
152000
-
native molecular mass of TTHB246-TTHB247 complex, determined by static light scattering Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q53WH9
-
-

Purification (Commentary)

Purification (Comment) Organism
purified to homogeneity using Ni2+-NTA chromatography Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + CoA + NAD+
-
Thermus thermophilus acetyl-CoA + NADH + H+
-
?
propionaldehyde + CoA + NAD+
-
Thermus thermophilus propanoyl-CoA + NADH + H+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 33000, SDS-PAGE, enzyme can form a stable heterotetrameric complex with TTHB246 in vitro, consisting of two aldolase and two dehydrogenase subunits Thermus thermophilus

Synonyms

Synonyms Comment Organism
TTHB247
-
Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life in complex with TTHB246 is 5.9 h Thermus thermophilus
50
-
half-life of chimeric BphI-TTHB247 complex is 0.54 h for BphI activity and 2.47 for TTHB247 activity Thermus thermophilus
50
-
half-life of single enzyme is 1.6 h Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2
-
acetaldehyde BphJ-TTHB246 complex, pH 8.0, 25°C Thermus thermophilus
2.3
-
propionaldehyde BphJ-TTHB246 complex, pH 8.0, 25°C Thermus thermophilus
2.6
-
propionaldehyde TTHB247-BphI complex, pH 8.0, 25°C Thermus thermophilus
2.8
-
acetaldehyde TTHB247-BphI complex, pH 8.0, 25°C Thermus thermophilus
3.5
-
acetaldehyde TTHB246-TTHB247 complex, pH 8.0, 25°C Thermus thermophilus
3.6
-
propionaldehyde TTHB246-TTHB247 complex, pH 8.0, 25°C Thermus thermophilus
7.4
-
acetaldehyde single TTHB247, pH 8.0, 25°C Thermus thermophilus
8.4
-
propionaldehyde single TTHB247, pH 8.0, 25°C Thermus thermophilus
16.3
-
propionaldehyde BphJ-BphI complex, pH 8.0, 25°C Thermus thermophilus
17.2
-
acetaldehyde BphJ-BphI complex, pH 8.0, 25°C Thermus thermophilus

General Information

General Information Comment Organism
metabolism together with TTHB246, EC 4.1.3.43, the enzyme forms a bacterial aldolase-dehydrogenase complex catalyzing the last steps in the meta cleavage pathway of aromatic hydrocarbon degradation Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.14
-
acetaldehyde BphJ-TTHB246 complex, pH 8.0, 25°C Thermus thermophilus
0.14
-
propionaldehyde BphJ-TTHB246 complex, pH 8.0, 25°C Thermus thermophilus
0.22
-
propionaldehyde TTHB246-TTHB247 complex, pH 8.0, 25°C Thermus thermophilus
0.23
-
acetaldehyde TTHB246-TTHB247 complex, pH 8.0, 25°C Thermus thermophilus
0.27
-
propionaldehyde TTHB247-BphI complex, pH 8.0, 25°C Thermus thermophilus
0.3
-
acetaldehyde TTHB247-BphI complex, pH 8.0, 25°C Thermus thermophilus
0.7
-
propionaldehyde BphJ-BphI complex, pH 8.0, 25°C Thermus thermophilus
0.73
-
acetaldehyde BphJ-BphI complex, pH 8.0, 25°C Thermus thermophilus
1.31
-
propionaldehyde single TTHB247, pH 8.0, 25°C Thermus thermophilus
1.35
-
acetaldehyde single TTHB247, pH 8.0, 25°C Thermus thermophilus