Cloned (Comment) | Organism |
---|---|
chimeric complexes of Burkholderia xenovorans and Thermus thermophilus enzymes, TTHB246-BphJ and BphI-TTHB247 created by coexpression of the relevant genes in Escherichia coli using separate expression plasmids | Thermus thermophilus |
separate expression of TTHB247 in recombinant Escherichia coli | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
acetaldehyde | app. Km-value, single TTHB247, pH 8.0, 25°C | Thermus thermophilus | |
6.4 | - |
propionaldehyde | app. Km-value, single TTHB247, pH 8.0, 25°C | Thermus thermophilus | |
9.4 | - |
acetaldehyde | app. Km-value, TTHB247-BphI complex, pH 8.0, 25°C | Thermus thermophilus | |
9.6 | - |
propionaldehyde | app. Km-value, TTHB247-BphI complex, pH 8.0, 25°C | Thermus thermophilus | |
15.4 | - |
acetaldehyde | app. Km-value, TTHB246-TTHB247 complex, pH 8.0, 25°C | Thermus thermophilus | |
15.7 | - |
acetaldehyde | app. Km-value, BphJ-TTHB246 complex, pH 8.0, 25°C | Thermus thermophilus | |
16.1 | - |
propionaldehyde | app. Km-value, BphJ-TTHB246 complex, pH 8.0, 25°C | Thermus thermophilus | |
16.1 | - |
propionaldehyde | app. Km-value, TTHB246-TTHB247 complex, pH 8.0, 25°C | Thermus thermophilus | |
23.1 | - |
propionaldehyde | app. Km-value, BphJ-BphI complex, pH 8.0, 25°C | Thermus thermophilus | |
23.6 | - |
acetaldehyde | app. Km-value, BphJ-BphI complex, pH 8.0, 25°C | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
estimated by SDS-PAGE, in agreement with predicted molecular mass calculated from amino acid sequences | Thermus thermophilus |
33000 | - |
native molecular mass of TTHB247, determined by static light scattering | Thermus thermophilus |
40000 | - |
native molecular mass of TTHB247, determined by gel filtration | Thermus thermophilus |
137000 | - |
molecular mass of BphI-TTHB247 chimeric complex, determined by static light scattering | Thermus thermophilus |
142000 | - |
native molecular mass of TTHB246-TTHB247 complex, determined by gel filtration | Thermus thermophilus |
144000 | - |
molecular mass of BphI-TTHB247 chimeric complex, determined by gel filtration | Thermus thermophilus |
152000 | - |
native molecular mass of TTHB246-TTHB247 complex, determined by static light scattering | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q53WH9 | - |
- |
Purification (Comment) | Organism |
---|---|
purified to homogeneity using Ni2+-NTA chromatography | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + CoA + NAD+ | - |
Thermus thermophilus | acetyl-CoA + NADH + H+ | - |
? | |
propionaldehyde + CoA + NAD+ | - |
Thermus thermophilus | propanoyl-CoA + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000, SDS-PAGE, enzyme can form a stable heterotetrameric complex with TTHB246 in vitro, consisting of two aldolase and two dehydrogenase subunits | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
TTHB247 | - |
Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life in complex with TTHB246 is 5.9 h | Thermus thermophilus |
50 | - |
half-life of chimeric BphI-TTHB247 complex is 0.54 h for BphI activity and 2.47 for TTHB247 activity | Thermus thermophilus |
50 | - |
half-life of single enzyme is 1.6 h | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
acetaldehyde | BphJ-TTHB246 complex, pH 8.0, 25°C | Thermus thermophilus | |
2.3 | - |
propionaldehyde | BphJ-TTHB246 complex, pH 8.0, 25°C | Thermus thermophilus | |
2.6 | - |
propionaldehyde | TTHB247-BphI complex, pH 8.0, 25°C | Thermus thermophilus | |
2.8 | - |
acetaldehyde | TTHB247-BphI complex, pH 8.0, 25°C | Thermus thermophilus | |
3.5 | - |
acetaldehyde | TTHB246-TTHB247 complex, pH 8.0, 25°C | Thermus thermophilus | |
3.6 | - |
propionaldehyde | TTHB246-TTHB247 complex, pH 8.0, 25°C | Thermus thermophilus | |
7.4 | - |
acetaldehyde | single TTHB247, pH 8.0, 25°C | Thermus thermophilus | |
8.4 | - |
propionaldehyde | single TTHB247, pH 8.0, 25°C | Thermus thermophilus | |
16.3 | - |
propionaldehyde | BphJ-BphI complex, pH 8.0, 25°C | Thermus thermophilus | |
17.2 | - |
acetaldehyde | BphJ-BphI complex, pH 8.0, 25°C | Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
metabolism | together with TTHB246, EC 4.1.3.43, the enzyme forms a bacterial aldolase-dehydrogenase complex catalyzing the last steps in the meta cleavage pathway of aromatic hydrocarbon degradation | Thermus thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
acetaldehyde | BphJ-TTHB246 complex, pH 8.0, 25°C | Thermus thermophilus | |
0.14 | - |
propionaldehyde | BphJ-TTHB246 complex, pH 8.0, 25°C | Thermus thermophilus | |
0.22 | - |
propionaldehyde | TTHB246-TTHB247 complex, pH 8.0, 25°C | Thermus thermophilus | |
0.23 | - |
acetaldehyde | TTHB246-TTHB247 complex, pH 8.0, 25°C | Thermus thermophilus | |
0.27 | - |
propionaldehyde | TTHB247-BphI complex, pH 8.0, 25°C | Thermus thermophilus | |
0.3 | - |
acetaldehyde | TTHB247-BphI complex, pH 8.0, 25°C | Thermus thermophilus | |
0.7 | - |
propionaldehyde | BphJ-BphI complex, pH 8.0, 25°C | Thermus thermophilus | |
0.73 | - |
acetaldehyde | BphJ-BphI complex, pH 8.0, 25°C | Thermus thermophilus | |
1.31 | - |
propionaldehyde | single TTHB247, pH 8.0, 25°C | Thermus thermophilus | |
1.35 | - |
acetaldehyde | single TTHB247, pH 8.0, 25°C | Thermus thermophilus |