BRENDA - Enzyme Database
show all sequences of 1.2.1.87

Investigating the molecular determinants for substrate channeling in BphI-BphJ, an aldolase-dehydrogenase complex from the polychlorinated biphenyls degradation pathway

Carere, J.; Baker, P.; Seah, S.Y.K.; Biochemistry 50, 8407-8416 (2011)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
bphI and bphJ cloned into the plasmids pBTL4-T7 and pET28a
Paraburkholderia xenovorans
Engineering
Protein Variants
Commentary
Organism
I195F
created by site-specific mutagenesis, no significant reduction of channeling efficiency of the enzyme complex toward acetaldehyde or propionaldehyde
Paraburkholderia xenovorans
I195L
created by site-specific mutagenesis
Paraburkholderia xenovorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
3
propionaldehyde
wild-type protein, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
7.4
-
acetaldehyde
I195L mutant, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
7.7
-
propionaldehyde
I195L mutant, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
24
-
acetaldehyde
wild-type protein, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
35
-
acetaldehyde
I195F mutant, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
48
-
propionaldehyde
I195F mutant, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
Organism
Organism
UniProt
Commentary
Textmining
Paraburkholderia xenovorans
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
BphI and BphJ form a stable complex as they bind and coelute from Ni2+-NTA column, after purification N-terminal histidine tag of BphJ is proteolytically cleaved by thrombin digestion
Paraburkholderia xenovorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
acetaldehyde + CoA + NAD+
-
721651
Paraburkholderia xenovorans
acetyl-CoA + NADH + H+
-
-
-
?
propionaldehyde + CoA + NAD+
-
721651
Paraburkholderia xenovorans
propanoyl-CoA + NADH + H+
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
BphJ
-
Paraburkholderia xenovorans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8.9
-
propionaldehyde
I195F mutant, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
16
-
acetaldehyde
I195F mutant, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
16.3
-
propionaldehyde
wild-type protein, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
17.2
-
acetaldehyde
wild-type protein, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
42
-
acetaldehyde
I195L mutant, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
46
-
propionaldehyde
I195L mutant, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
Cloned(Commentary) (protein specific)
Commentary
Organism
bphI and bphJ cloned into the plasmids pBTL4-T7 and pET28a
Paraburkholderia xenovorans
Engineering (protein specific)
Protein Variants
Commentary
Organism
I195F
created by site-specific mutagenesis, no significant reduction of channeling efficiency of the enzyme complex toward acetaldehyde or propionaldehyde
Paraburkholderia xenovorans
I195L
created by site-specific mutagenesis
Paraburkholderia xenovorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2
3
propionaldehyde
wild-type protein, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
7.4
-
acetaldehyde
I195L mutant, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
7.7
-
propionaldehyde
I195L mutant, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
24
-
acetaldehyde
wild-type protein, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
35
-
acetaldehyde
I195F mutant, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
48
-
propionaldehyde
I195F mutant, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
Purification (Commentary) (protein specific)
Commentary
Organism
BphI and BphJ form a stable complex as they bind and coelute from Ni2+-NTA column, after purification N-terminal histidine tag of BphJ is proteolytically cleaved by thrombin digestion
Paraburkholderia xenovorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
acetaldehyde + CoA + NAD+
-
721651
Paraburkholderia xenovorans
acetyl-CoA + NADH + H+
-
-
-
?
propionaldehyde + CoA + NAD+
-
721651
Paraburkholderia xenovorans
propanoyl-CoA + NADH + H+
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
8.9
-
propionaldehyde
I195F mutant, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
16
-
acetaldehyde
I195F mutant, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
16.3
-
propionaldehyde
wild-type protein, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
17.2
-
acetaldehyde
wild-type protein, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
42
-
acetaldehyde
I195L mutant, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
46
-
propionaldehyde
I195L mutant, steady state, pH 8.0, 25°C
Paraburkholderia xenovorans
General Information
General Information
Commentary
Organism
metabolism
enzyme is part of the BphI-BphJ-complex, an aldolase-dehydrogenase-complex from the polychlorinated biphenyl degradation pathway
Paraburkholderia xenovorans
General Information (protein specific)
General Information
Commentary
Organism
metabolism
enzyme is part of the BphI-BphJ-complex, an aldolase-dehydrogenase-complex from the polychlorinated biphenyl degradation pathway
Paraburkholderia xenovorans
Other publictions for EC 1.2.1.87
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742372
Lan
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1
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-
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-
2
1
1
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-
1
10
-
-
-
-
-
-
-
-
-
1
-
-
-
-
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-
-
9
-
-
6
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1
-
-
-
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2
1
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1
10
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-
-
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1
1
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10
10
721651
Carere
Investigating the molecular de ...
Paraburkholderia xenovorans
Biochemistry
50
8407-8416
2011
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1
-
2
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-
6
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-
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
6
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
6
-
-
-
-
-
1
1
-
-
-
721615
Baker
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Paraburkholderia xenovorans
Biochemistry
48
6551-6558
2009
-
-
1
-
-
-
-
6
-
-
3
1
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1
-
-
1
-
-
-
-
1
8
1
1
-
-
-
6
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
6
-
-
3
1
-
-
-
1
-
-
-
1
8
1
-
-
-
6
-
-
-
-
-
1
1
-
7
7