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Search Purification (Commentary)

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EC Number Purification (Commentary)
Show all pathways known for 1.2.1.87Display the reaction diagram Show all sequences 1.2.1.87BphI and BphJ form a stable complex as they bind and coelute from Ni2+-NTA column, after purification N-terminal histidine tag of BphJ is proteolytically cleaved by thrombin digestion
Show all pathways known for 1.2.1.87Display the reaction diagram Show all sequences 1.2.1.87BphI and BphJ form a stable complex as they bind and coelute from Ni2+-NTA column, although only BphJ has the histidine tag. After purification, the N-terminal histidine tag of BphJ is proteolytically cleaved by thrombin digestion
Show all pathways known for 1.2.1.87Display the reaction diagram Show all sequences 1.2.1.87purified to homogeneity using Ni2+-NTA chromatography
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