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2'-deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
2'-deoxyuridine + phosphate
uracil + deoxyribose 1-phosphate
5'-deoxy-5-fluorouridine + alpha-D-ribose 1-phosphate
5'-deoxy-5-fluorouridine + phosphate
-
-
-
-
?
5'-deoxy-5-fluorouridine + phosphate
5-fluorouracil + 5-deoxyribose-1-phosphate
5-bromo-2'-deoxyuridine + phosphate
5-bromouracil + 2-deoxribose 1-phosphate
5-bromouracil + deoxyribose 1-phosphate
5-bromodeoxyuridine + phosphate
5-bromouridine + phosphate
5-bromouracil + alpha-D-ribose 1-phosphate
5-fluoro-2'-deoxyuridine + phosphate
5-fluorouracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
5-fluoro-2'-deoxyuridine + phosphate
5-fluorouracil + 2-deoxy-D-ribose-1-phosphate
-
-
also accepts 2'-deoxypyrimidine nucleosides in higher organisms
-
r
5-fluoro-2'-deoxyuridine + phosphate
5-fluorouracil + 2-deoxyribose-1-phosphate
5-fluorouracil + alpha-D-ribose 1-phosphate
5-fluorouridine + phosphate
-
-
-
-
?
5-fluorouracil + alpha-D-ribose-1-phosphate
5-fluorouridine + phosphate
5-fluorouridine + phosphate
5-fluorouracil + alpha-D-ribose 1-phosphate
5-fluorouridine + phosphate
5-fluorouracil + alpha-D-ribose-1-phosphate
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
5-methyluridine + phosphate
thymine + alpha-D-ribose 1-phosphate
-
19% of the activity with uridine
-
-
?
6-methyluracil + 2-deoxy-D-ribose 1-phosphate
6-methyl-2'-deoxyuridine + phosphate
-
VchUPh shows selectivity toward 6-methyluracil acting as a pseudosubstrate. Repulsion between the hydrophilic hydroxyl group of the active-site residue Thr93 of VchUPh and the hydrophobic methyl group of 6-methyluracil prevents the oxygen atom O4' of the ribose moiety and the phosphate oxygen atom O3P of ribose 1-phosphate from forming hydrogen bonds with OG1_Thr93, which are essential for the enzymatic reaction. This, apparently, renders VchUPh inactive in the enzymatic synthesis of 6-methyluridine from 6-methyluracil
-
-
ir
6-methyluracil + alpha-D-ribose 1-phosphate
6-methyluridine + phosphate
-
VchUPh shows selectivity toward 6-methyluracil acting as a pseudosubstrate. Repulsion between the hydrophilic hydroxyl group of the active-site residue Thr93 of VchUPh and the hydrophobic methyl group of 6-methyluracil prevents the oxygen atom O4' of the ribose moiety and the phosphate oxygen atom O3P of ribose 1-phosphate from forming hydrogen bonds with OG1_Thr93, which are essential for the enzymatic reaction. This, apparently, renders VchUPh inactive in the enzymatic synthesis of 6-methyluridine from 6-methyluracil
-
-
ir
6-methyluridine + phosphate
6-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
?
arabinofuranosyl-5-ethyluracil + phosphate
5-ethyluracil + arabinose-1-phosphate
-
weak substrate
-
-
?
azathymine + deoxyribose 1-phosphate
azadeoxythymidine + phosphate
-
-
-
-
ir
azauracil + deoxyribose 1-phosphate
azadeoxyuridine + phosphate
-
-
-
-
?
azauracil + ribose 1-phosphate
azauridine + phosphate
-
-
-
-
ir
cytidine + phosphate
cytosine + alpha-D-ribose 1-phosphate
-
-
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-alpha-D-ribose 1-phosphate
deoxyuridine + phosphate
uracil + deoxy-D-ribose 1-phosphate
fluorouridine + phosphate
fluorouracil + alpha-D-ribose-1-phosphate
guanosine + phosphate
guanine + alpha-D-ribose 1-phosphate
10% of the activity with uridine
-
-
r
inosine + phosphate
hypoxanthine + alpha-D-ribose 1-phosphate
-
-
-
?
pyrimidine nucleoside + phosphate
pyrimidine base + alpha-D-ribose 1-phosphate
-
most pyrimidine nucleosides except for 4-amino-substituted species like deoxycytidine
or alpha-D-deoxyribose 1-phosphate
-
r
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
thymine + deoxyribose 1-phosphate
deoxythymidine + phosphate
-
-
-
-
?
thymine + ribose 1-phosphate
thymidine + phosphate
-
-
-
-
?
uracil + alpha-D-ribose 1-phosphate
uridine + phosphate
uracil arabinoside + phosphate
uracil + arabinose-1-phosphate
uridine + arsenate
?
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
additional information
?
-
2'-deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
?
2'-deoxyuridine + phosphate
uracil + 2-deoxy-D-ribose 1-phosphate
-
-
-
?
2'-deoxyuridine + phosphate
uracil + deoxyribose 1-phosphate
-
6% of the activity with uridine
-
?
2'-deoxyuridine + phosphate
uracil + deoxyribose 1-phosphate
-
activities of uridine, deoxyuridine and thymidine phosphorylase from Giardia lamblia remain associated throughout purification suggesting that a single enzyme is responsible for the 3 activities
-
-
?
2'-deoxyuridine + phosphate
uracil + deoxyribose 1-phosphate
-
12% of the activity with uridine
-
-
?
2'-deoxyuridine + phosphate
uracil + deoxyribose 1-phosphate
-
-
-
-
?
2'-deoxyuridine + phosphate
uracil + deoxyribose 1-phosphate
-
18% of the activity with uridine
-
-
?
2'-deoxyuridine + phosphate
uracil + deoxyribose 1-phosphate
-
-
-
-
?
2'-deoxyuridine + phosphate
uracil + deoxyribose 1-phosphate
-
catalyzed by a different protein or by a different active center of the same enzyme
-
r
2'-deoxyuridine + phosphate
uracil + deoxyribose 1-phosphate
-
unlike cytosolic enzyme, enzyme from plasma membranes shows little or no deoxyuridine-cleaving activity
-
-
?
2'-deoxyuridine + phosphate
uracil + deoxyribose 1-phosphate
-
-
-
-
?
2'-deoxyuridine + phosphate
uracil + deoxyribose 1-phosphate
-
-
-
-
r
5'-deoxy-5-fluorouridine + phosphate
5-fluorouracil + 5-deoxyribose-1-phosphate
-
-
-
-
?
5'-deoxy-5-fluorouridine + phosphate
5-fluorouracil + 5-deoxyribose-1-phosphate
-
25% of the activity with uridine
-
-
?
5-bromo-2'-deoxyuridine + phosphate
5-bromouracil + 2-deoxribose 1-phosphate
-
27% of the activity with uridine
-
-
?
5-bromo-2'-deoxyuridine + phosphate
5-bromouracil + 2-deoxribose 1-phosphate
-
75% of the activity with uridine
-
-
?
5-bromouracil + deoxyribose 1-phosphate
5-bromodeoxyuridine + phosphate
-
-
-
-
?
5-bromouracil + deoxyribose 1-phosphate
5-bromodeoxyuridine + phosphate
-
-
-
-
?
5-bromouridine + phosphate
5-bromouracil + alpha-D-ribose 1-phosphate
-
69% of the activity with uridine
-
-
?
5-bromouridine + phosphate
5-bromouracil + alpha-D-ribose 1-phosphate
-
40% of the activity with uridine
-
-
?
5-bromouridine + phosphate
5-bromouracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
5-fluoro-2'-deoxyuridine + phosphate
5-fluorouracil + 2-deoxyribose-1-phosphate
-
14% of the activity with uridine
-
-
?
5-fluoro-2'-deoxyuridine + phosphate
5-fluorouracil + 2-deoxyribose-1-phosphate
-
15% of the activity with uridine
-
-
?
5-fluorouracil + alpha-D-ribose-1-phosphate
5-fluorouridine + phosphate
-
-
-
-
?
5-fluorouracil + alpha-D-ribose-1-phosphate
5-fluorouridine + phosphate
-
-
-
-
?
5-fluorouridine + phosphate
5-fluorouracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
5-fluorouridine + phosphate
5-fluorouracil + alpha-D-ribose 1-phosphate
-
-
-
-
r
5-fluorouridine + phosphate
5-fluorouracil + alpha-D-ribose-1-phosphate
-
-
-
?
5-fluorouridine + phosphate
5-fluorouracil + alpha-D-ribose-1-phosphate
-
15% of the activity with uridine
-
-
?
5-fluorouridine + phosphate
5-fluorouracil + alpha-D-ribose-1-phosphate
-
85% of the activity with uridine
-
-
?
5-fluorouridine + phosphate
5-fluorouracil + alpha-D-ribose-1-phosphate
-
-
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
92% compared to the activity with uridine
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
92% compared to the activity with uridine
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
27% of the activity with uridine
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
?
5-methyluridine + phosphate
5-methyluracil + alpha-D-ribose 1-phosphate
-
-
-
-
r
deoxyuridine + phosphate
uracil + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
deoxyuridine + phosphate
uracil + 2-deoxy-alpha-D-ribose 1-phosphate
14% of the activity with uridine
-
-
r
deoxyuridine + phosphate
uracil + deoxy-D-ribose 1-phosphate
66% compared to the activity with uridine
-
-
?
deoxyuridine + phosphate
uracil + deoxy-D-ribose 1-phosphate
66% compared to the activity with uridine
-
-
?
deoxyuridine + phosphate
uracil + deoxy-D-ribose 1-phosphate
-
-
-
r
fluorouridine + phosphate
fluorouracil + alpha-D-ribose-1-phosphate
-
-
-
-
?
fluorouridine + phosphate
fluorouracil + alpha-D-ribose-1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
60% compared to the activity with uridine
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
60% compared to the activity with uridine
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
2% of the activity with uridine
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
the activities of uridine, deoxyuridine and thymidine phosphorylases from Giardia lamblia remain associated throughout purification, suggesting that a single enzyme is responsible for the 3 activities
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
21% of the activity with uridine
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
to a lesser extent compared to uridine
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
22% of the activity with uridine
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
to a lesser extent compared to uridine
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
?
thymidine + phosphate
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
-
-
-
r
uracil + alpha-D-ribose 1-phosphate
uridine + phosphate
-
-
-
-
?
uracil + alpha-D-ribose 1-phosphate
uridine + phosphate
-
-
-
r
uracil + alpha-D-ribose 1-phosphate
uridine + phosphate
-
-
-
-
?
uracil + alpha-D-ribose 1-phosphate
uridine + phosphate
-
uridine prevents the glucose deprivation-induced death of immunostimulated astrocytes via the action of uridine phosphorylase
-
-
?
uracil arabinoside + phosphate
uracil + arabinose-1-phosphate
-
10% of the activity with uridine
-
-
?
uracil arabinoside + phosphate
uracil + arabinose-1-phosphate
-
10% of the activity with uridine
-
-
?
uracil arabinoside + phosphate
uracil + arabinose-1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
the optimal substrate for the enzyme is uridine. It shows no activity for 5-fluorouridine, cytidine, or 2-deoxycytidine
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
the optimal substrate for the enzyme is uridine. It shows no activity for 5-fluorouridine, cytidine, or 2-deoxycytidine
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
key enzyme in pyrimidine-salvage pathway
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
liver enzyme is highly specific to uridine
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
equilibrium position favouring nucleoside synthesis
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
broader specificity than human enzyme
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
enzyme of pyrimidine salvage pathway
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
in conjunction with uridine kinase, the enzyme provides a route for the conversion of uracil to UMP via uridine
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
role in degradation of pyrimidine nucleosides as well as in the salvage pathway for nucleic acid synthesis
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
cleavage of the N-glycosidic bond
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
enzyme of pyrimidine salvage pathway
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
best substrate, active site structure, overview
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
r
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
-
?
uridine + phosphate
uracil + alpha-D-ribose 1-phosphate
-
-
-
?
additional information
?
-
-
no substrate: cytidine
-
-
?
additional information
?
-
-
no substrates are: thymidine, deoxyuridine, fluorodeoxyuridiune, 5'-deoxyfluorouridine
-
-
?
additional information
?
-
-
no substrate: 5-substituted-2,2'-anhydrouridine
-
-
?
additional information
?
-
-
catalyzes the first step of uridine degradation to beta-alanine in liver
-
-
?
additional information
?
-
UPP catalyzes the reversible phosphorolysis of uracil ribosides and analogous compounds to their respective nucleobases and ribose-1-phosphate, structural mechanisms, overview
-
-
?
additional information
?
-
-
UPP catalyzes the reversible phosphorolysis of uracil ribosides and analogous compounds to their respective nucleobases and ribose-1-phosphate, structural mechanisms, overview
-
-
?
additional information
?
-
-
no substrate: cytidine
-
-
?
additional information
?
-
-
no substrate: cytidine
-
-
?
additional information
?
-
-
no substrates: 2-deoxyuridine, adenosine
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
no substrate: 5-substituted-2,2'-anhydrouridine
-
-
?
additional information
?
-
-
the enzyme is regulated by multiple lipid-sensing nuclear receptors, gene expression is diurnally regulated
-
-
?
additional information
?
-
-
no substrates: 3'-azido-2',3'-dideoxy-5-methyluridine, 3'-azido-2',3'-dideoxy-5-ethyluridine, 2',3'-dideoxy-5-ethyluridine, 3'-chloro-2',3'-dideoxy-5-ethyluridine, 3'-chloro-2',3'-dideoxy-5-methyluridine, 3'-bromo-2',3'-dideoxy-5-ethyluridine, 2'-deoxylyxofuranosyl-5-ethyluracil, 3'-O-acetyl-2,2'-anhydro-5-ethyluridine, 2,3'-anhydro-2'-deoxy-5-ethyluridine, 2,5'-anhydro-2'-deoxy-5-ethyluridine
-
-
?
additional information
?
-
-
catalyzes the first step of uridine degradation to beta-alanine in liver
-
-
?
additional information
?
-
-
no substrates: inosine, adenosine, guanosine, thymidine
-
-
?
additional information
?
-
-
the enzyme also activates pyrimidine-containing drugs, including 5-fluorouracil, mechanism of the enzyme-drug interaction, overview
-
-
?
additional information
?
-
-
no substrate: cytidine
-
-
?
additional information
?
-
-
no substrates: cytosine, deoxycytidine, orotidine
-
-
?
additional information
?
-
strict substrate specificty of the enzyme, overview. Deoxyadenosine, and deoxyguanosine are poor substrates, thymidine, cytidine, deoxycytidine, inosine, and adenosine are no substrates
-
-
?
additional information
?
-
-
strict substrate specificty of the enzyme, overview. Deoxyadenosine, and deoxyguanosine are poor substrates, thymidine, cytidine, deoxycytidine, inosine, and adenosine are no substrates
-
-
?
additional information
?
-
-
substrate specificity, and substrate binding structures, detailed overview
-
-
-
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1-((2-pyrrolidine-1-yl)ethyl)uracil
inhibits both enzymic activity and growth of Plasmodium falciparum
1-(1',2'-dihydroxypropyl)-5,6-tetramethyleneuracil
-
only the R-enantiomer inhibits, but not the S-enantiomer
1-(1',3'-dihydroxy-2'-propoxy)methyl-5,6-tetramethyleneuracil
-
inhibits forward and reverse reaction
1-(1',3'-dihydroxy-2'-propoxy)methyl-5-benzyluracil
-
i.e. DHPBU, competitive
2',3'-Dideoxy-5-ethyluridine
-
-
2'-deoxyglycosylthymine
-
-
2'-deoxylyxofuranosyl-5-ethyluracil
-
-
2,2'-Anhydro-5-ethyluridine
2,3'-Anhydro-2'-deoxy-5-ethyluridine
-
-
2,3'-anhydro-5-ethyluridine
-
competitive
2,5'-Anhydro-2'-deoxy-5-ethyluridine
-
-
2-deoxyglycosylthymine
-
-
3'-Azido-2',3'-dideoxy-5-ethyluridine
-
-
3'-Azido-2',3'-dideoxy-5-methyluridine
-
-
3'-Bromo-2',3'-dideoxy-5-ethyluridine
-
-
3'-Chloro-2',3'-dideoxy-5-ethyluridine
-
-
3'-Chloro-2',3'-dideoxy-5-methyluridine
-
-
3'-O-Acetyl-2,2'-anhydro-5-ethyluridine
-
-
3-((2-pyrrolidine-1-yl)ethyl)uracil
inhibits both enzymic activity and growth of Plasmodium falciparum
3-O-methyl-alpha-D-glucopyranose
-
-
4-benzyl-6-hydroxy-2-oxo-1,2-dihydropyridine-3-carbonitrile
2% inhibition at 0.001 mM
4-ethyl-6-hydroxy-2-oxo-1,2-dihydropyridine-3-carbonitrile
25% inhibition at 0.001 mM
4-hydroxy-5-[[(2-hydroxyethyl)amino]methyl]-3,4-dihydropyrimidin-2(1H)-one
2% inhibition at 0.001 mM
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
5-(3'-Benzyloxybenzyl)-1-[(1'-aminomethyl-2'-hydroxyethoxy)methyl]uracil
5-(Benzyloxybenzyloxybenzyl)acyluridine
-
-
-
5-(phenylthio) acyclouridine
-
5-(phenylthio)acyclouridine
5-Azauracil
-
reaction is inhibited in the direction of deoxyuridine synthesis more than in the direction of their cleavage at the same concentration of 5-azauracil
5-benzyl-1-(2'-hydroxyethoxymethyl)uracil
competitive inhibition with respect to uridine, noncompetitive inhibition with respect to phosphate
5-benzyl-6-hydroxy-4-methyl-2-oxo-1,2-dihydropyridine-3-carbonitrile
43% inhibition at 0.001 mM
5-fluoroacyclouridine
-
-
5-m-benzyloxybenzyl acyclouridine
-
5-m-benzyloxybenzyl barbituric acid acyclonucleoside
-
5-phenylselenenylacyclouridine
-
5-phenylthioacyclouridine
-
5-Substituted 2,2'-anhydrouridine
5-[(diethylamino)methyl]-6-hydroxy-4-methyl-2-oxo-1,2-dihydropyridine-3-carbonitrile
25% inhibition at 0.001 mM
5-[(dimethylamino)methyl]-6-hydroxy-4-methyl-2-oxo-1,2-dihydropyridine-3-carbonitrile
81% inhibition at 0.001 mM
5-[[(1,3-dihydroxypropan-2-yl)amino]methyl]-6-hydroxy-4-methyl-2-oxo-1,2-dihydropyridine-3-carbonitrile
82% inhibition at 0.001 mM
5-[[(2,3-dihydroxypropyl)amino]methyl]-6-hydroxy-4-methyl-2-oxo-1,2-dihydropyridine-3-carbonitrile
78% inhibition at 0.001 mM
6-hydroxy-1H-pyridin-2-one
38% inhibition at 0.001 mM
6-hydroxy-2-oxo-1,2-dihydropyridine-3-carbonitrile
67% inhibition at 0.001 mM
6-hydroxy-2-oxo-4-(propan-2-yl)-1,2-dihydropyridine-3-carbonitrile
31% inhibition at 0.001 mM
6-hydroxy-2-oxo-4-phenyl-1,2-dihydropyridine-3-carbonitrile
42% inhibition at 0.001 mM
6-hydroxy-2-oxo-4-propyl-1,2-dihydropyridine-3-carbonitrile
16% inhibition at 0.001 mM
6-hydroxy-3,4-dimethylpyridin-2(1H)-one
9.5% inhibition at 0.001 mM
6-hydroxy-3-methylpyridin-2(1H)-one
12% inhibition at 0.001 mM
6-hydroxy-4-methyl-1H-pyridin-2-one-3-carbonitrile
-
6-hydroxy-4-methyl-2-oxo-1,2-dihydropyridine-3-carbonitrile
70% inhibition at 0.001 mM
6-hydroxy-4-methyl-2-oxo-5-phenyl-1,2-dihydropyridine-3-carbonitrile
12% inhibition at 0.001 mM
6-hydroxy-4-methyl-2-oxo-5-[(piperidin-1-yl)methyl]-1,2-dihydropyridine-3-carbonitrile
49% inhibition at 0.001 mM
6-hydroxy-4-methyl-5-[(morpholin-4-yl)methyl]-2-oxo-1,2-dihydropyridine-3-carbonitrile
51% inhibition at 0.001 mM
6-hydroxy-4-methylpyridin-2(1H)-one
16% inhibition at 0.001 mM
6-hydroxy-4-propyl-1H-pyridin-2-one-3-carbonitrile
16% inhibition at 0.001 mM
6-hydroxy-5-(((2-hydroxyethyl) amino) methyl)-4-methyl-1H-pyridin-2-one-3-carbonitrile
-
6-hydroxy-5-(((2-hydroxyethyl)amino)methyl)-4-methyl-1H-pyridin-2-one-3-carbonitrile
80% inhibition at 0.001 mM
Acyclonucleoside analogues
-
consisting of 5- and 5,6-substituted uracils and different acyclic chains
-
Acyclothymidine
-
competitive
acyclouridine
-
competitive
alpha-D-ribose 1-phosphate
Arabinofuranosyl-5-ethyluracil
-
-
Deoxyglucosylthymine
-
phosphorolysis of uridine and deoxyuridine, synthesis of uridine at concentrations of 0.10 mM, 0.018 mM and 0.14 mM, not: phosphorolysis of deoxyuridine or thymidine at 0.19 mM
guanidine hydrochloride
-
50% loss of activity at 1.04 M, only little residual activity between 2 and 6 M
N-ethyl-5-phenylisoxazolium-3'-sulfonate
-
Woodward's reagent K
p-Mercuriphenylsulfonate
-
-
potassium 5-cyano-4-methyl-6-oxo-1,6-dihydropyridin-2-olate
60% inhibition at 0.001 mM
Pyrimidine acyclonucleosides
-
competitive
-
tetramethylene acyclouridine
-
-
thymidine 5-monophosphate
-
-
2,2'-Anhydro-5-ethyluridine
-
-
2,2'-Anhydro-5-ethyluridine
-
0.001 mM, competitive
2,2'-Anhydro-5-ethyluridine
-
-
2,2'-Anhydro-5-ethyluridine
-
competitive
2,2'-Anhydro-5-ethyluridine
-
-
2,2'-Anhydro-5-ethyluridine
-
-
2,2'-anhydrouridine
-
high-resolution three-dimensional structure of uridine phosphorylase from in complex with the competitive inhibitor determined by X-ray diffraction (PDB ID 6RCA), detailed binding structure analysis, overview. The binding of the inhibitor at the active site of the protein is energetically more favorable than the binding of the native substrate uridine. The inhibitor molecules are located in all six active sites of the hexameric UPh molecule. Effect of 2,2'-anhydrouridine binding on the conformation of structurally functional elements of VchUPh
2,2'-anhydrouridine
competitive inhibitor
5-(3'-Benzyloxybenzyl)-1-[(1'-aminomethyl-2'-hydroxyethoxy)methyl]uracil
-
-
5-(3'-Benzyloxybenzyl)-1-[(1'-aminomethyl-2'-hydroxyethoxy)methyl]uracil
-
-
5-(phenylthio)acyclouridine
-
specific inhibitor
5-(phenylthio)acyclouridine
-
-
5-(phenylthio)acyclouridine
-
specific inhibitor
5-benzylacyclouridine
-
5-benzylacyclouridine
-
0.01 mM, 90% inhibition of normal tissues, 40-60% inhibition of carcinoma tissue
5-benzylacyclouridine
-
-
5-benzylacyclouridine
an UPP1-specific inhibitor. Binding of the inhibitor to the UPP1 active site, structure, overview
5-benzylacyclouridine
BAU
5-fluoro-2'-deoxyuridine
-
-
5-fluoro-2'-deoxyuridine
-
-
5-Substituted 2,2'-anhydrouridine
-
-
5-Substituted 2,2'-anhydrouridine
-
-
6-Methyluracil
-
-
6-Methyluracil
-
acts as a pseudosubstrate, molecular docking into the active site, molecular-dynamics simulations of the complex. Repulsion between the hydrophilic hydroxyl group of the active-site residue Thr93 of VchUPh and the hydrophobic methyl group of 6-methyluracil prevents the oxygen atom O4' of the ribose moiety and the phosphate oxygen atom O3P of ribose 1-phosphate from forming hydrogen bonds with OG1_Thr93, which are essential for the enzymatic reaction. This, apparently, renders VchUPh inactive in the enzymatic synthesis of 6-methyluridine from 6-methyluracil
alpha-D-ribose 1-phosphate
product inhibition pattern, noncompetitive versus uridine, competitive versus phosphate, overview
alpha-D-ribose 1-phosphate
-
-
alpha-D-ribose 1-phosphate
-
product inhibition, noncompetitive with respect to uridine and phosphate
Benzylacyclouridines
-
0.1 mM
-
Benzylacyclouridines
-
-
-
Benzylacyclouridines
-
0.1 mM
-
Benzylacyclouridines
-
-
-
Benzylacyclouridines
-
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
phosphate
-
-
phosphate
-
product inhibition
phosphate
-
substrate inhibition above 10 mM
ribose 1-phosphate
-
-
ribose 1-phosphate
-
product inhibition
ribose 1-phosphate
-
inhibition above 0.6 mM
Uracil
-
product inhibition
Uracil
product inhibition pattern, noncompetitive versus uridine and phosphate, overview
Uracil
-
product inhibition, oncompetitive with respect to uridine and phosphate
uridine
-
product inhibition
uridine
-
substrate inhibition
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Properties of nucleoside phosphorylase from Enterobacter aerogenes
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49
3239-3246
1985
Klebsiella aerogenes, Klebsiella aerogenes AJ 11125
-
brenda
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1964
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Purification and characterization of uridine (thymidine) phosphorylase from Giardia lamblia
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1988
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Correlation of substrate-stabilization patterns with proposed mechanisms for three nucleoside phosphorylases
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703
247-249
1982
Escherichia coli
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Purification and characterization of uridine and thymidine phosphorylase from Lactobacillus casei
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1040
287-293
1990
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Thymidine phosphorylase and uridine phosphorylase of Lactobacillus casei
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56
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Lacticaseibacillus casei
-
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2002
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178
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1988
Rattus norvegicus
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58
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Uridine and deoxyuridine phosphorylases from Ehrlich ascites tumor
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Pyrimidine metabolism. II. Enzymatic pathways of uracil anabolism
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Pyrimidine acyclonucleosides, inhibitors of uridine phosphorylase
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122
397-404
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5-Substituted-2,2-anhydrouridines, potent inhibitors of uridine phosphorylase
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34
1737-1740
1985
Homo sapiens, Mus musculus
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New analogues of benzylacyclouridines, specific and potent inhibitors of uridine phosphorylase from human and mouse livers
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36
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Homo sapiens, Mus musculus
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Purification and kinetic characterization of uridine phosphorylase from Dictyostelium discoiderm
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13
13-19
1989
Dictyostelium discoideum
-
brenda
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Isolation, crystallization in the macrogravitation field, preliminary X-ray investigation of uridine phosphorylase from Escherichia coli K-12
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26
607-615
1992
Escherichia coli
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A one-step procedure for the purification of uridine phosphorylase from Escherichia coli
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133
153-156
1983
Escherichia coli, Escherichia coli B / ATCC 11303
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226
687-692
1983
Escherichia coli, Escherichia coli B / ATCC 11303
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Crystallization and preliminary X-ray investigation of uridine phosphorylase from Escherichia coli
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2852-2853
1987
Escherichia coli
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Uridine phosphorylase from Escherichia coli. Kinetic properties and mechanism
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352-358
1976
Escherichia coli
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42c
288-296
1987
Escherichia coli, Escherichia coli B / ATCC 11303
-
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Uridine phosphorylase from rat liver
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1978
Rattus norvegicus
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Uridine phosphorylase, molecular properties and mechanism of catalysis
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2051-2056
1974
Rattus norvegicus
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Uridine phosphorylase activity of isolated plasma membranes of rat liver
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Rattus norvegicus
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Uridine phosphorylase from Escherichia coli. Physical and chemical characterization
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Escherichia coli
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Uridine phosphorylase from Schistosoma mansoni
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6081-6086
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Schistosoma mansoni
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Purification and substrate specificity of pyrimidine nucleoside phosphorylase from Haemophilus influenzae
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Acholeplasma laidlawii
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62
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1997
Escherichia coli
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Complete inactivation of Escherichia coli uridine phosphorylase by modification of Asp5 with Woodward's reagent K
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Escherichia coli
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Saccharomyces cerevisiae URH1 (encoding uridine-cytidine N-ribohydrolase): functional complementation by a nucleoside hydrolase from a protozoan parasite and by a mammalian uridine phosphorylase
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Isolation and initial characterization of the uridine phosphorylase from Salmonella typhimurium
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Salmonella enterica subsp. enterica serovar Typhimurium
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Uridine phosphorylase association with vimentin. Intracellular distribution and localization
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Purification, cloning, and expression of murine uridine phosphorylase
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Structure of Escherichia coli uridine phosphorylase at 2.0 A
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Purification, crystallization and preliminary X-ray analysis of uridine phosphorylase from Salmonella typhimurium
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Structural basis for inhibition of Escherichia coli uridine phosphorylase by 5-substituted acyclouridines
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Preliminary investigation of the three-dimensional structure of Salmonella typhimurium uridine phosphorylase in the crystalline state
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F61
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Johansson, M.
Identification of a novel human uridine phosphorylase
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307
41-46
2003
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Synthesis and evaluation of 6-methylene-bridged uracil derivatives. Part 1: discovery of novel orally active inhibitors of human thymidine phosphorylase
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12
3431-3441
2004
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Oliva, I.; Zuffi, G.; Orsini, G.; Tonon, G.; de Gioia, L.; Ghisotti, D.
Mutagenesis of Escherichia coli uridine phosphorylase by random pentapeptide insertions
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35
309-314
2004
Escherichia coli
-
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Oliva, I.; Zuffi, G.; Barile, D.; Orsini, G.; Tonon, G.; De Gioia, L.; Ghisotti, D.
Characterization of Escherichia coli uridine phosphorylase by single-site mutagenesis
J. Biochem.
135
495-499
2004
Escherichia coli (P12758), Escherichia coli
brenda
Caradoc-Davies, T.T.; Cutfield, S.M.; Lamont, I.L.; Cutfield, J.F.
Crystal structures of Escherichia coli uridine phosphorylase in two native and three complexed forms reveal basis of substrate specificity, induced conformational changes and influence of potassium
J. Mol. Biol.
337
337-354
2004
Escherichia coli (P12758), Escherichia coli
brenda
Zhang, Y.; Repa, J.J.; Inoue, Y.; Hayhurst, G.P.; Gonzalez, F.J.; Mangelsdorf, D.J.
Identification of a liver-specific uridine phosphorylase that is regulated by multiple lipid-sensing nuclear receptors
Mol. Endocrinol.
18
851-862
2004
Mus musculus
brenda
Yan, R.; Wan, L.; Pizzorno, G.; Cao, D.
Uridine phosphorylase in breast cancer: a new prognostic factor?
Front. Biosci.
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2759-2766
2006
Gallus gallus, Homo sapiens, Rattus norvegicus
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Temmink, O.H.; de Bruin, M.; Laan, A.C.; Turksma, A.W.; Cricca, S.; Masterson, A.J.; Noordhuis, P.; Peters, G.J.
The role of thymidine phosphorylase and uridine phosphorylase in (fluoro)pyrimidine metabolism in peripheral blood mononuclear cells
Int. J. Biochem. Cell Biol.
38
1759-1765
2006
Homo sapiens
brenda
Temmink, O.H.; de Bruin, M.; Turksma, A.W.; Cricca, S.; Laan, A.C.; Peters, G.J.
Activity and substrate specificity of pyrimidine phosphorylases and their role in fluoropyrimidine sensitivity in colon cancer cell lines
Int. J. Biochem. Cell Biol.
39
565-575
2007
Homo sapiens
brenda
Wan, L.; Cao, D.; Zeng, J.; Yan, R.; Pizzorno, G.
Modulation of uridine phosphorylase gene expression by tumor necrosis factor-alpha enhances the antiproliferative activity of the capecitabine intermediate 5-deoxy-5-fluorouridine in breast cancer cells
Mol. Pharmacol.
69
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2006
Mus musculus
brenda
Balestri, F.; Barsotti, C.; Lutzemberger, L.; Camici, M.; Ipata, P.L.
Key role of uridine kinase and uridine phosphorylase in the homeostatic regulation of purine and pyrimidine salvage in brain
Neurochem. Int.
51
517-523
2007
Homo sapiens, Rattus norvegicus
brenda
Choi, J.W.; Yoo, B.K.; Shin, C.Y.; Ryu, M.K.; Ryu, J.H.; el Kouni, M.H.; Lee, J.C.; Kim, W.K.; Ko, K.H.
Uridine prevents the glucose deprivation-induced death of immunostimulated astrocytes via the action of uridine phosphorylase
Neurosci. Res.
56
111-118
2006
Rattus norvegicus
brenda
Timofeev, V.I.; Lashkov, A.A.; Gabdoulkhakov, A.G.; Pavlyuk, B.P.; Kachalova, G.S.; Betzel, C.; Morgunova, E.Y.; Zhukhlistova, N.E.; Mikhailov, A.M.
Isolation, crystallization and preliminary crystallographic analysis of Salmonella typhimurium uridine phosphorylase crystallized with 2,2-anhydrouridine
Acta Crystallogr. Sect. F
63
852-854
2007
Salmonella enterica subsp. enterica serovar Typhimurium
brenda
Kawamura, K.; Takiguchi, N.; Wada, A.; Takenobu, H.; Kimura, H.; Soda, H.; Nagata, M.; Asano, T.; Tagawa, M.
Up-regulated expression of the uridine phosphorylase gene in human gastric tumors is correlated with a favorable prognosis
Anticancer Res.
26
4647-4651
2007
Homo sapiens
brenda
Choi, J.W.; Shin, C.Y.; Choi, M.S.; Yoon, S.Y.; Ryu, J.H.; Lee, J.C.; Kim, W.K.; El Kouni, M.H.; Ko, K.H.
Uridine protects cortical neurons from glucose deprivation-induced death: possible role of uridine phosphorylase
J. Neurotrauma
25
695-707
2008
Mus musculus, Rattus norvegicus
brenda
Fujimoto, K.; Matsumura, Y.; Tani, Y.; Ozono, S.; Hirao, Y.; Okajima, E.
Tissue levels of pyrimidine nucleoside phosphorylase activity in human and rodent bladder cancer and normal bladder tissue
Int. J. Urol.
14
754-759
2007
Mus musculus, Rattus norvegicus
brenda
Lashkov, A.A.; Gabdoulkhakov, A.G.; Shtil, A.A.; Mikhailov, A.M.
Crystallization and preliminary X-ray c uridine phosphorylase complexed with 5-fluorouracil
Acta Crystallogr. Sect. F
65
601-603
2009
Salmonella enterica subsp. enterica serovar Typhimurium
brenda
Renck, D.; Ducati, R.G.; Palma, M.S.; Santos, D.S.; Basso, L.A.
The kinetic mechanism of human uridine phosphorylase 1: Towards the development of enzyme inhibitors for cancer chemotherapy
Arch. Biochem. Biophys.
497
35-42
2010
Homo sapiens (Q16831), Homo sapiens
brenda
Roosild, T.P.; Castronovo, S.; Fabbiani, M.; Pizzorno, G.
Implications of the structure of human uridine phosphorylase 1 on the development of novel inhibitors for improving the therapeutic window of fluoropyrimidine chemotherapy
BMC Struct. Biol.
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14
2009
Homo sapiens (Q16831), Homo sapiens
brenda
Larson, E.T.; Mudeppa, D.G.; Gillespie, J.R.; Mueller, N.; Napuli, A.J.; Arif, J.A.; Ross, J.; Arakaki, T.L.; Lauricella, A.; Detitta, G.; Luft, J.; Zucker, F.; Verlinde, C.L.; Fan, E.; Van Voorhis, W.C.; Buckner, F.S.; Rathod, P.K.; Hol, W.G.; Merritt, E.A.
The crystal structure and activity of a putative trypanosomal nucleoside phosphorylase reveal it to be a homodimeric uridine phosphorylase
J. Mol. Biol.
396
1244-1259
2010
Trypanosoma brucei (Q57VZ2), Trypanosoma brucei
brenda
Kong, X.; Fan, H.; Liu, X.; Wang, R.; Liang, J.; Gupta, N.; Chen, Y.; Fang, F.; Chang, Y.
Peroxisome proliferator-activated receptor gamma coactivator-1alpha enhances antiproliferative activity of 5-deoxy-5-fluorouridine in cancer cells through induction of uridine phosphorylase
Mol. Pharmacol.
76
854-860
2009
Homo sapiens
brenda
Lashkov, A.A.; Zhukhlistova, N.E.; Gabdoulkhakov, A.H.; Shtil, A.A.; Efremov, R.G.; Betzel, C.; Mikhailov, A.M.
The X-ray structure of Salmonella typhimurium uridine nucleoside phosphorylase complexed with 2,2-anhydrouridine, phosphate and potassium ions at 1.86 A resolution
Acta Crystallogr. Sect. D
66
51-60
2010
Salmonella enterica subsp. enterica serovar Typhimurium (P0A1F6), Salmonella enterica subsp. enterica serovar Typhimurium
brenda
Lashkov, A.A.; Sotnichenko, S.E.; Prokofiev, I.I.; Gabdulkhakov, A.G.; Agapov, I.I.; Shtil, A.A.; Betzel, C.; Mironov, A.S.; Mikhailov, A.M.
X-ray structure of Salmonella typhimurium uridine phosphorylase complexed with 5-fluorouracil and molecular modelling of the complex of 5-fluorouracil with uridine phosphorylase from Vibrio cholerae
Acta Crystallogr. Sect. D
68
968-974
2012
Vibrio cholerae serotype O1, Salmonella enterica subsp. enterica serovar Typhimurium (P0A1F6), Salmonella enterica subsp. enterica serovar Typhimurium
brenda
Safonova, T.N.; Mordkovich, N.N.; Polyakov, K.M.; Manuvera, V.A.; Veiko, V.P.; Popov, V.O.
Crystallization of uridine phosphorylase from Shewanella oneidensis MR-1 in the laboratory and under microgravity and preliminary X-ray diffraction analysis
Acta Crystallogr. Sect. F
68
1387-1389
2012
Shewanella oneidensis (C7EWM3), Shewanella oneidensis MR-1 / ATCC 700550 (C7EWM3)
brenda
Lashkov, A.A.; Gabdulkhakov, A.G.; Prokofev, I.I.; Seregina, T.A.; Sotnichenko, S.E.; Lyashenko, A.V.; Shtil, A.A.; Mironov, A.S.; Betzel, C.; Mikhailov, A.M.
Expression, purification, crystallization and preliminary X-ray structure analysis of Vibrio cholerae uridine phosphorylase in complex with thymidine
Acta Crystallogr. Sect. F
68
1394-1397
2012
Vibrio cholerae (Q9K4U1), Vibrio cholerae
brenda
Silva, R.G.; Schramm, V.L.
Uridine phosphorylase from Trypanosoma cruzi: kinetic and chemical mechanisms
Biochemistry
50
9158-9166
2011
Trypanosoma cruzi
brenda
Silva, R.G.; Kipp, D.R.; Schramm, V.L.
Constrained bonding environment in the Michaelis complex of Trypanosoma cruzi uridine phosphorylase
Biochemistry
51
6715-6717
2012
Trypanosoma cruzi
brenda
Zhu, S.; Ren, L.; Wang, J.; Zheng, G.; Tang, P.
Two-step efficient synthesis of 5-methyluridine via two thermostable nucleoside phosphorylase from Aeropyrum pernix
Bioorg. Med. Chem. Lett.
22
2102-2104
2012
Aeropyrum pernix (Q9YA34), Aeropyrum pernix
brenda
Lashkov, A.; Zhukhlistova, N.; Sotnichenko, S.; Gabdulkhakov, A.; Mikhailov, A.
Structural basis for the mechanism of inhibition of uridine phosphorylase from Salmonella typhimurium
Crystallogr. Rep.
55
41-57
2010
Salmonella enterica subsp. enterica serovar Typhimurium (P0A1F6)
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brenda
Cui, H.; Ruda, G.F.; Carrero-Lerida, J.; Ruiz-Perez, L.M.; Gilbert, I.H.; Gonzalez-Pacanowska, D.
Exploring new inhibitors of Plasmodium falciparum purine nucleoside phosphorylase
Eur. J. Med. Chem.
45
5140-5149
2010
Plasmodium falciparum (Q8I3X4), Plasmodium falciparum
brenda
Visser, D.; Hennessy, F.; Rashamuse, J.; Pletschke, B.; Brady, D.
Stabilization of Escherichia coli uridine phosphorylase by evolution and immobilization
J. Mol. Catal. B
68
279-285
2011
Escherichia coli
-
brenda
Roosild, T.P.; Castronovo, S.; Villoso, A.; Ziemba, A.; Pizzorno, G.
A novel structural mechanism for redox regulation of uridine phosphorylase 2 activity
J. Struct. Biol.
176
229-237
2011
Homo sapiens (O95045), Homo sapiens
brenda
Roosild, T.; Castronovo, S.
Active site conformational dynamics in human uridine phosphorylase 1
PLoS ONE
5
e12741
2010
Homo sapiens (Q16831), Homo sapiens
brenda
Zhu, S.; Song, D.; Gong, C.; Tang, P.; Li, X.; Wang, J.; Zheng, G.
Biosynthesis of nucleoside analogues via thermostable nucleoside phosphorylase
Appl. Microbiol. Biotechnol.
97
6769-6778
2013
Aeropyrum pernix (Q9YA34), Aeropyrum pernix, Aeropyrum pernix DSM 11879 (Q9YA34)
brenda
Safonova, T.N.; Mikhailov, S.N.; Veiko, V.P.; Mordkovich, N.N.; Manuvera, V.A.; Alekseev, C.S.; Kovalchuk, M.V.; Popov, V.O.; Polyakov, K.M.
High-syn conformation of uridine and asymmetry of the hexameric molecule revealed in the high-resolution structures of Shewanella oneidensis MR-1 uridine phosphorylase in the free form and in complex with uridine
Acta Crystallogr. Sect. D
70
3310-3319
2014
Shewanella oneidensis (C7EWM3), Shewanella oneidensis MR-1 / ATCC 700550 (C7EWM3)
brenda
Safonova, T.N.; Mordkovich, N.N.; Veiko, V.P.; Okorokova, N.A.; Manuvera, V.A.; Dorovatovskii, P.V.; Popov, V.O.; Polyakov, K.M.
Concerted action of two subunits of the functional dimer of Shewanella oneidensis MR-1 uridine phosphorylase derived from a comparison of the C212S mutant and the wild-type enzyme
Acta Crystallogr. Sect. D
72
203-210
2016
Shewanella oneidensis (C7EWM3), Shewanella oneidensis MR-1 / ATCC 700550 (C7EWM3)
brenda
Prokofev, I.I.; Lashkov, A.A.; Gabdulkhakov, A.G.; Dontsova, M.V.; Seregina, T.A.; Mironov, A.S.; Betzel, C.; Mikhailov, A.M.
Crystallization and preliminary X-ray study of Vibrio cholerae uridine phosphorylase in complex with 6-methyluracil
Acta Crystallogr. Sect. F
70
60-63
2014
Vibrio cholerae (Q9K4U1), Vibrio cholerae
brenda
da Silva Neto, A.M.; Torini de Souza, J.R.; Romanello, L.; Cassago, A.; Serrao, V.H.; DeMarco, R.; Brandao-Neto, J.; Garratt, R.C.; Pereira, H.D.
Analysis of two Schistosoma mansoni uridine phosphorylases isoforms suggests the emergence of a protein with a non-canonical function
Biochimie
125
12-22
2016
Schistosoma mansoni (G4VGH9), Schistosoma mansoni
brenda
Lashkov, A.; Sotnichenko, S.; Mikhailov, A.
In silico analysis of the three-dimensional structures of the homodimer of uridine phosphorylase from Yersinia pseudotuberculosis in the ligand-free state and in a complex with 5-fluorouracil
Crystallogr. Rep.
58
280-286
2013
Yersinia pseudotuberculosis
-
brenda
Balaev, V.; Lashkov, A.; Gabdulkhakov, A.; Dontsova, M.; Mironov, A.; Betzel, C.; Mikhailov, A.
Three-dimensional structures of unligated uridine phosphorylase from Yersinia pseudotuberculosis at 1.4 resolution and its complex with an antibacterial drug
Crystallogr. Rep.
60
525-531
2015
Yersinia pseudotuberculosis (Q7AZX0)
-
brenda
Mordkovich, N.; Safonova, T.; Manuvera, V.; Veiko, V.; Polyakov, K.; Alekseev, K.; Mikhailov, S.; Popov, V.
Physicochemical characterization of uridine phosphorylase from Shewanella oneidensis MR-1
Dokl. Biochem. Biophys.
451
187-189
2013
Shewanella oneidensis (C7EWM3), Shewanella oneidensis MR-1 / ATCC 700550 (C7EWM3)
brenda
Renck, D.; Santos, A.A.; Machado, P.; Petersen, G.O.; Lopes, T.G.; Santos, D.S.; Campos, M.M.; Basso, L.A.
Human uridine phosphorylase-1 inhibitors: a new approach to ameliorate 5-fluorouracil-induced intestinal mucositis
Invest. New Drugs
32
1301-1307
2014
Rattus norvegicus (Q499V1), Rattus norvegicus
brenda
Xiong, S.; Wang, Y.; Wang, X.; Wang, J.; Li, J.; Zhang, G.; Zhang, R.; Xie, L.; Wang, H.
Enzymatic synthesis of 2-deoxyuridine by whole cell catalyst co-expressing uridine phosphorylase and thymidine phosphorylase through auto-induction system
J. Biosci. Bioeng.
118
723-727
2014
Escherichia coli
brenda
Renck, D.; Machado, P.; Souto, A.A.; Rosado, L.A.; Erig, T.; Campos, M.M.; Farias, C.B.; Roesler, R.; Timmers, L.F.; de Souza, O.N.; Santos, D.S.; Basso, L.A.
Design of novel potent inhibitors of human uridine phosphorylase-1: synthesis, inhibition studies, thermodynamics, and in vitro influence on 5-fluorouracil cytotoxicity
J. Med. Chem.
56
8892-8902
2013
Homo sapiens (Q16831), Homo sapiens
brenda
Ritt, J.; Raymond, F.; Leprohon, P.; Lgar, D.; Corbeil, J.; Ouellette, M.
Gene amplification and point mutations in pyrimidine metabolic genes in 5-fluorouracil resistant Leishmania infantum
PLoS Negl. Trop. Dis.
7
e2564
2013
Leishmania infantum, Leishmania infantum MHOM/MA/67/ITMAP-263
brenda
Mordkovich, N.; Safonova, T.; Antipov, A.; Manuvera, V.; Polyakov, K.; Okorokova, N.; Veiko, V.
Study of structural-functional organization of nucleoside phosphorylases of gammaproteobacteria. Special aspects of functioning of uridine phosphorylase phosphate-binding site
Appl. Biochem. Microbiol.
54
12-20
2018
Shewanella oneidensis (Q8E927)
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brenda
Wang, J.; Xu, S.; Lv, W.; Shi, F.; Mei, S.; Shan, A.; Xu, J.; Yang, Y.
Uridine phosphorylase 1 is a novel immune-related target and predicts worse survival in brain glioma
Cancer Med.
9
5940-5947
2020
Homo sapiens (Q16831), Homo sapiens
brenda
Prokofev, I.; Lashkov, A.; Gabdulkhakov, A.; Balaev, V.; Seregina, T.; Mironov, A.; Betzel, C.; Mikhailov, A.
X-ray structures of uridine phosphorylase from Vibrio cholerae in complexes with uridine, thymidine, uracil, thymine, and phosphate anion substrate specificity of bacterial uridine phosphorylases
Crystallogr. Rep.
61
954-973
2016
Vibrio cholerae
-
brenda
Prokofev, I.; Lashkov, A.; Gabdulkhakov, A.; Balaev, V.; Mironov, A.; Betzel, C.; Mikhailov, A.
Structural and functional analysis of pyrimidine nucleoside phosphorylases of the NP-I and NP-II families in complexes with 6-methyluracil
Crystallogr. Rep.
63
418-427
2018
Vibrio cholerae
-
brenda
Eistrikh-Heller, P.; Rubinsky, S.; Prokofev, I.; Gabdulkhakov, A.; Mironov, A.; Lashkov, A.
X-ray structure and molecular dynamics study of uridine phosphorylase from Vibrio cholerae in complex with 2,2-anhydrouridine
Crystallogr. Rep.
65
269-277
2020
Vibrio cholerae
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brenda
Guan, Y.; Bhandari, A.; Zhang, X.; Wang, O.
Uridine phosphorylase 1 associates to biological and clinical significance in thyroid carcinoma cell lines
J. Cell. Mol. Med.
23
7438-7448
2019
Homo sapiens (Q16831)
brenda
Yang, C.; Li, J.; Huang, Z.; Zhang, X.; Gao, X.; Zhu, C.; Morris, P.F.; Zhang, X.
Structural and catalytic analysis of two diverse uridine phosphorylases in Phytophthora capsici
Sci. Rep.
10
9051
2020
no activity in Hyaloperonospora arabidopsidis, no activity in Albugo spp., no activity in Saprolegnia spp., no activity in Aphanomyces spp., no activity in Bremia lactuca, Phytophthora capsici (A0A410UCT3), Phytophthora capsici (A0A6G6VYG7), Phytophthora capsici, Phytophthora capsici LT1534 (A0A410UCT3), Phytophthora capsici LT1534 (A0A6G6VYG7)
brenda