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Literature summary for 2.4.2.3 extracted from
- Uridine phosphorylase, molecular properties and mechanism of catalysis (1974), Biochemistry, 13, 2051-2056.
General Stability
| General Stability | Organism |
|---|---|
| much less stable in Tris buffer than in phosphate buffer | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ribose 1-phosphate | inhibition above 0.6 mM | Rattus norvegicus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
4 * 26000, SDS-PAGE in presence of 6 M urea, without 2-mercaptoethanol | Rattus norvegicus |
| 102500 | - |
disc gel electrophoresis | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| uridine + phosphate = uracil + alpha-D-ribose 1-phosphate | ordered bi-bi mechanism | Rattus norvegicus | |
| uridine + phosphate = uracil + alpha-D-ribose 1-phosphate | phosphate binds before uridine and ribose 1-phosphate is released after uracil | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 26000, SDS-PAGE in presence of 6 M urea, without 2-mercaptoethanol | Rattus norvegicus |
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Release 2023.1 (January 2023)
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