KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22200 | - |
8 * 22200, SDS-PAGE | Escherichia coli |
160000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
homogeneity | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
133 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxyuridine + phosphate | 6% of the activity with uridine | Escherichia coli | uracil + deoxyribose 1-phosphate | - |
? | |
5-bromo-2'-deoxyuridine + phosphate | 27% of the activity with uridine | Escherichia coli | 5-bromouracil + 2-deoxribose 1-phosphate | - |
? | |
5-bromouridine + phosphate | 69% of the activity with uridine | Escherichia coli | 5-bromouracil + alpha-D-ribose 1-phosphate | - |
? | |
5-fluoro-2'-deoxyuridine + phosphate | 14% of the activity with uridine | Escherichia coli | 5-fluorouracil + 2-deoxyribose-1-phosphate | - |
? | |
5-methyluridine + phosphate | 19% of the activity with uridine | Escherichia coli | thymine + alpha-D-ribose 1-phosphate | - |
? | |
thymidine + phosphate | 2% of the activity with uridine | Escherichia coli | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | - |
Escherichia coli | uracil + alpha-D-ribose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 22200, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
uridine phosphorolysis | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 9 | pH 5.8: about 50% of activity maximum, pH 9.0: about 40% of activity maximum | Escherichia coli |