Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain AM201 | Vibrio cholerae |
Crystallization (Comment) | Organism |
---|---|
in complex with 6-methyluracil, hanging drop vapor diffusion method, using 15% (w/v) PEG 4000, 0.1 M MgCl2, 0.1 M Tris-HCl pH 8.5 | Vibrio cholerae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27500 | - |
6 * 27500, calculated from amino acid sequence | Vibrio cholerae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uridine + phosphate | Vibrio cholerae | - |
uracil + alpha-D-ribose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae | Q9K4U1 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, butyl Sepharose column chromatography, and Q Sepharose column chromatography | Vibrio cholerae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-methyluridine + phosphate | - |
Vibrio cholerae | 6-methyluracil + alpha-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | - |
Vibrio cholerae | uracil + alpha-D-ribose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 27500, calculated from amino acid sequence | Vibrio cholerae |
Synonyms | Comment | Organism |
---|---|---|
UPH | - |
Vibrio cholerae |