Information on EC 2.1.1.188 - 23S rRNA (guanine748-N1)-methyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.188
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RECOMMENDED NAME
GeneOntology No.
23S rRNA (guanine748-N1)-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + guanine748 in 23S rRNA = S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:23S rRNA (guanine748-N1)-methyltransferase
The enzyme specifically methylates guanine748 at N1 in 23S rRNA. The methyltransferase RlmAII confers resistance to the macrolide antibiotic tylosin in the drug-producing strain Streptomyces fradiae [1].
CAS REGISTRY NUMBER
COMMENTARY hide
50812-25-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
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Manually annotated by BRENDA team
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
-
Manually annotated by BRENDA team
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
-
Manually annotated by BRENDA team
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
-
Manually annotated by BRENDA team
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
-
Manually annotated by BRENDA team
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
-
Manually annotated by BRENDA team
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
-
Manually annotated by BRENDA team
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
-
Manually annotated by BRENDA team
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
show the reaction diagram
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methyluanine748 in 23S rRNA
show the reaction diagram
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in vivo TlrB modifies nucleotide G748 within helix 35 of 23S rRNA
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-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
show the reaction diagram
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methyluanine748 in 23S rRNA
show the reaction diagram
-
in vivo TlrB modifies nucleotide G748 within helix 35 of 23S rRNA
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-
?
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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methylation of the adjacent uridine at position 747 by RlmCD enhances guanine748 methylation by the enzyme
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31500
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x * 31500, calculated from amino acid sequence
32000
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x * 32000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
glutathione Sepharose column chromatography and Superdex 75 gel filtration; glutathione Sepharose column chromatography and Superdex75 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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expression in Escherichia coli
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introduction of tlrB into Streptomyces lividans confers tylosin resistance
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
RlmCD disruption does not influence the level of the enzyme
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A135T
-
inactive
L113P
-
inactive
L164P
-
inactive
L165P
-
inactive
L247P
-
inactive
R200C
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inactive
Y212C
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inactive
A135T
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inactive
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C23R
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inactive
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C36R
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inactive
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L113P
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inactive
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Y212C
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inactive
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