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Literature summary for 2.1.1.188 extracted from

  • Liu, M.; Douthwaite, S.
    Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes gram-negative from gram-positive bacteria (2002), Mol. Microbiol., 44, 195-204.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Bacillus subtilis
-
Streptococcus pneumoniae
-
Corynebacterium glutamicum
-
Micromonospora griseorubida
-
Streptomyces fradiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine748 in 23S rRNA Priestia megaterium Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Bacillus megaterium shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Enterococcus faecalis Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterococcus faecalis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Lactiplantibacillus plantarum Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactobacillus plantarum shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Lactococcus lactis Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactococcus lactis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Rhodococcus corynebacterioides Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Nocardia corynebacteroides shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Streptococcus thermophilus Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Streptococcus thermophilus shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Bacillus subtilis Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Streptococcus pneumoniae Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Corynebacterium glutamicum Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Micromonospora griseorubida Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Streptomyces fradiae Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Corynebacterium glutamicum
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Enterococcus faecalis
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Lactiplantibacillus plantarum
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Lactococcus lactis
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Micromonospora griseorubida
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Priestia megaterium
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Rhodococcus corynebacterioides
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Streptococcus pneumoniae
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Streptococcus thermophilus
-
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-
Streptomyces fradiae Q9S1M6 comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis
-
Streptococcus pneumoniae
-
Corynebacterium glutamicum
-
Micromonospora griseorubida
-
Streptomyces fradiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Bacillus megaterium shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro Priestia megaterium S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterococcus faecalis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro Enterococcus faecalis S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactobacillus plantarum shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro Lactiplantibacillus plantarum S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactococcus lactis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro Lactococcus lactis S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Nocardia corynebacteroides shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro Rhodococcus corynebacterioides S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Streptococcus thermophilus shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro Streptococcus thermophilus S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Bacillus subtilis S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Streptococcus pneumoniae S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Corynebacterium glutamicum S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Micromonospora griseorubida S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate Streptomyces fradiae S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?

Synonyms

Synonyms Comment Organism
RlmAII
-
Lactiplantibacillus plantarum
RlmAII
-
Bacillus subtilis
RlmAII
-
Priestia megaterium
RlmAII
-
Lactococcus lactis
RlmAII
-
Enterococcus faecalis
RlmAII
-
Streptococcus pneumoniae
RlmAII
-
Streptococcus thermophilus
RlmAII
-
Micromonospora griseorubida
RlmAII
-
Rhodococcus corynebacterioides
RlmAII
-
Streptomyces fradiae
rRNA large subunit methyltransferase II
-
Lactiplantibacillus plantarum
rRNA large subunit methyltransferase II
-
Bacillus subtilis
rRNA large subunit methyltransferase II
-
Priestia megaterium
rRNA large subunit methyltransferase II
-
Lactococcus lactis
rRNA large subunit methyltransferase II
-
Enterococcus faecalis
rRNA large subunit methyltransferase II
-
Streptococcus pneumoniae
rRNA large subunit methyltransferase II
-
Streptococcus thermophilus
rRNA large subunit methyltransferase II
-
Micromonospora griseorubida
rRNA large subunit methyltransferase II
-
Rhodococcus corynebacterioides
rRNA large subunit methyltransferase II
-
Streptomyces fradiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Lactiplantibacillus plantarum
37
-
assay at Bacillus subtilis
37
-
assay at Priestia megaterium
37
-
assay at Lactococcus lactis
37
-
assay at Enterococcus faecalis
37
-
assay at Streptococcus pneumoniae
37
-
assay at Corynebacterium glutamicum
37
-
assay at Streptococcus thermophilus
37
-
assay at Micromonospora griseorubida
37
-
assay at Rhodococcus corynebacterioides
37
-
assay at Streptomyces fradiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Lactiplantibacillus plantarum
7.5
-
assay at Bacillus subtilis
7.5
-
assay at Priestia megaterium
7.5
-
assay at Lactococcus lactis
7.5
-
assay at Enterococcus faecalis
7.5
-
assay at Streptococcus pneumoniae
7.5
-
assay at Corynebacterium glutamicum
7.5
-
assay at Streptococcus thermophilus
7.5
-
assay at Micromonospora griseorubida
7.5
-
assay at Rhodococcus corynebacterioides
7.5
-
assay at Streptomyces fradiae