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Literature summary for 2.1.1.188 extracted from

  • Shoji, T.; Takaya, A.; Sato, Y.; Kimura, S.; Suzuki, T.; Yamamoto, T.
    RlmCD-mediated U747 methylation promotes efficient G748 methylation by methyltransferase RlmAII in 23S rRNA in Streptococcus pneumoniae; interplay between two rRNA methylations responsible for telithromycin susceptibility (2015), Nucleic Acids Res., 43, 8964-8972.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information methylation of the adjacent uridine at position 747 by RlmCD enhances guanine748 methylation by the enzyme Streptococcus pneumoniae

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptococcus pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
x * 32000, SDS-PAGE Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine748 in 23S rRNA Streptococcus pneumoniae
-
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA Streptococcus pneumoniae S1
-
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-
Streptococcus pneumoniae S1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography and Superdex 75 gel filtration Streptococcus pneumoniae
glutathione Sepharose column chromatography and Superdex75 gel filtration Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine748 in 23S rRNA
-
Streptococcus pneumoniae S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
-
Streptococcus pneumoniae S1 S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Streptococcus pneumoniae

Synonyms

Synonyms Comment Organism
RlmAII
-
Streptococcus pneumoniae

Expression

Organism Comment Expression
Streptococcus pneumoniae RlmCD disruption does not influence the level of the enzyme additional information

General Information

General Information Comment Organism
metabolism the enzyme enhances binding of telithromycin to A752 in ribosomes Streptococcus pneumoniae