Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | methylation of the adjacent uridine at position 747 by RlmCD enhances guanine748 methylation by the enzyme | Streptococcus pneumoniae |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptococcus pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Streptococcus pneumoniae | - |
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Streptococcus pneumoniae S1 | - |
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Streptococcus pneumoniae S1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography and Superdex 75 gel filtration | Streptococcus pneumoniae |
glutathione Sepharose column chromatography and Superdex75 gel filtration | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine748 in 23S rRNA | - |
Streptococcus pneumoniae | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | - |
Streptococcus pneumoniae S1 | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Streptococcus pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
RlmAII | - |
Streptococcus pneumoniae |
Organism | Comment | Expression |
---|---|---|
Streptococcus pneumoniae | RlmCD disruption does not influence the level of the enzyme | additional information |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme enhances binding of telithromycin to A752 in ribosomes | Streptococcus pneumoniae |