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2.6.1.2: alanine transaminase

This is an abbreviated version!
For detailed information about alanine transaminase, go to the full flat file.

Word Map on EC 2.6.1.2

Reaction

L-alanine
+
2-oxoglutarate
=
pyruvate
 +
L-glutamate

Synonyms

AAT, AKT, AlaAT, AlaAT1, AlaAT2, alanine aminotransferase, alanine aminotransferase 1, alanine aminotransferase-1, alanine transaminase, alanine-2-oxoglutarate aminotransferase, alanine-alpha-ketoglutarate aminotransferase, alanine-aminotransferase, alanine-pyruvate aminotransferase, alanine: 2-oxoglutarate aminotransferase, ALAT, ALT, ALT1, aminotransferase, alanine, At1g17290, AT3, beta-A:P TAm, beta-alanine aminotransferase, beta-alanine: pyruvate transaminase, Bh D-AlaAT, c-AAT, glutamate pyruvate 2-phosphotransferase, glutamate-pyruvate transaminase, glutamic acid-pyruvic acid transaminase, glutamic-alanine transaminase, glutamic-pyruvic aminotransferase, glutamic-pyruvic transaminase, GmAlaAT, GPT, HvAlaAT, L-alanine aminotransferase, L-alanine transaminase, L-alanine-alpha-ketoglutarate aminotransferase, LOC_Os10g25130, More, OsAlaAT, Pp beta-AlaAT, pyruvate transaminase, pyruvate-alanine aminotransferase, pyruvate-glutamate transaminase, Qsd1, Rp D-AlaAT, ScAlaAT, SkPyd4p, YLSs alanine aminotransferase, ZmAlaAT1, ZmAlaAT2

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.2 alanine transaminase

Purification

Purification on EC 2.6.1.2 - alanine transaminase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 minor forms: AlaAT-1, AlaAT-3, 1 major form, AlaAT-2, ammonium sulfate, DEAE-cellulose, Sephacryl S-200, phenyl-Sepharose
-
ammonium sulfate, DEAE-cellulose, 3 isoforms
-
ammonium sulfate, DEAE-Sephadex, Sephadex G-200, partial purification
-
ammonium sulfate, DEAE-Sepharose, partial purification of cytosolic and mitochondrial isoenzymes
-
ammonium sulfate, hydroxylapatite, preparative electrophoresis
-
ammonium sulfate, Sephadex G-150, DEAE-Sephadex, partial purification
-
anaerobically induced isoform, ammonium sulfate, gel filtration, Q-Sepharose, Mono P, Mono Q
-
DEAE-Sephadex
-
Dowex 50WX8 200-400 mesh chromatography
-
heat treatment, ammonium sulfate, DEAE-cellulose, isoelectric focusing, Sephadex G-150, hydroxylapatite, DEAE-cellulose
-
heat treatment, ammonium sulfate, DEAE-Sepharose, hydroxylapatite, Sephacryl S-200
-
HisTrap HP column chromatography
-
native enzyme, Q-Sepharose, phenyl-Sepharose, hydroxyapatite, S-Sepharose, Mono Q, Superdex 200, recombinant enzyme, heat treatment, Mono Q
Ni-agarose resin column chromatography
-
Ni-NTA agarose column chromatography
Ni2+-NTA agarose resin chromatography and Poly-Prep mini-column chromatography
-
recombinant enzyme
-
Sephadex G-150 column chromatography, DEAE-Sephadex A-25 column chromatography, and hydroxyapatite column chromatography
-
Sephadex G-150, DEAE-cellulose, ammonium sulfate, isoelectric focusing
-
Sepharose S12 gel filtration and nickel-chelating Sepharose column chromatography
using a DE52 column, butyl-Toyopearl column, DEAE-Toyopearl column, CHT ceramic hydroxyapatite column and MonoQ column
-
using anion exchange protein Pak Q 8HR column attached to HPLC system
-
using saline precipitation, gel filtration, preparative electrophoresis and anion exchange chromatography on protein-Pak Q 8HR column attached to HPLC
-