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Literature summary for 2.6.1.2 extracted from
- Glutamate-glyoxylate aminotransferase in rat liver cytosol. Purification, properties and identity with alanine-2-oxoglutarate aminotransferase (1977), Hoppe-Seyler's Z. Physiol. Chem., 358, 1533-1542.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Rattus norvegicus |  |
| hydroxylamine | 1 mM, 96% inhibition | Rattus norvegicus | |
| Isonicotinic hydrazide | - |
Rattus norvegicus | |
| KCN | - |
Rattus norvegicus | |
| Semicarbazide | 1 mM, 40% inhibition | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 112000 | - |
sucrose density gradient centrifugation | Rattus norvegicus |
| 112000 | 118000 | gel filtration | Homo sapiens |
| 112000 | 118000 | gel filtration | Canis lupus familiaris |
| 112000 | 118000 | gel filtration | Felis catus |
| 115000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canis lupus familiaris | - |
- |
- |
| Felis catus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| heat treatment, ammonium sulfate, DEAE-cellulose, isoelectric focusing, Sephadex G-150, hydroxylapatite, DEAE-cellulose | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
| liver | - |
Canis lupus familiaris | - |
| liver | - |
Felis catus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 480 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50 mM potassium phosphate buffer, pH 7.5, 5 mM 2-mercaptoethanol, 5% glycerol, stable for at least 5 weeks | Rattus norvegicus |
| 0-5°C, 50 mM potassium phosphate buffer, pH 7.5, 5 mM 2-mercaptoethanol, 5% glycerol, stable for 2 weeks | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glyoxylate + alanine | 70% of activity with glutamate | Rattus norvegicus | glycine + pyruvate | - |
ir | |
| glyoxylate + glutamate | - |
Rattus norvegicus | glycine + 2-oxoglutarate | - |
ir | |
| glyoxylate + glutamine | 42% of activity with glutamate | Rattus norvegicus | glycine + 2-oxosuccinamate | - |
? | |
| glyoxylate + methionine | 37% of activity with glutamate | Rattus norvegicus | glycine + 4-methylsulfanyl-2-oxobutanoate | - |
? | |
| L-alanine + 2-oxoglutarate | - |
Homo sapiens | pyruvate + L-glutamate | - |
? | |
| L-alanine + 2-oxoglutarate | - |
Canis lupus familiaris | pyruvate + L-glutamate | - |
? | |
| L-alanine + 2-oxoglutarate | - |
Felis catus | pyruvate + L-glutamate | - |
? | |
| L-alanine + 2-oxoglutarate | with 2-oxoglutarate specific for L-alanine | Rattus norvegicus | pyruvate + L-glutamate | - |
? | |
| L-alanine + 2-oxoglutarate | glutamate-glyoxylate aminotransferase is identical with alanine-2-oxoglutarate aminotransferase | Rattus norvegicus | pyruvate + L-glutamate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Rattus norvegicus |
| 37 | - |
assay at | Canis lupus familiaris |
| 37 | - |
assay at | Felis catus |
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