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2.6.1.2: alanine transaminase

This is an abbreviated version!
For detailed information about alanine transaminase, go to the full flat file.

Word Map on EC 2.6.1.2

Reaction

L-alanine
+
2-oxoglutarate
=
pyruvate
 +
L-glutamate

Synonyms

AAT, AKT, AlaAT, AlaAT1, AlaAT2, alanine aminotransferase, alanine aminotransferase 1, alanine aminotransferase-1, alanine transaminase, alanine-2-oxoglutarate aminotransferase, alanine-alpha-ketoglutarate aminotransferase, alanine-aminotransferase, alanine-pyruvate aminotransferase, alanine: 2-oxoglutarate aminotransferase, ALAT, ALT, ALT1, aminotransferase, alanine, At1g17290, AT3, beta-A:P TAm, beta-alanine aminotransferase, beta-alanine: pyruvate transaminase, Bh D-AlaAT, c-AAT, glutamate pyruvate 2-phosphotransferase, glutamate-pyruvate transaminase, glutamic acid-pyruvic acid transaminase, glutamic-alanine transaminase, glutamic-pyruvic aminotransferase, glutamic-pyruvic transaminase, GmAlaAT, GPT, HvAlaAT, L-alanine aminotransferase, L-alanine transaminase, L-alanine-alpha-ketoglutarate aminotransferase, LOC_Os10g25130, More, OsAlaAT, Pp beta-AlaAT, pyruvate transaminase, pyruvate-alanine aminotransferase, pyruvate-glutamate transaminase, Qsd1, Rp D-AlaAT, ScAlaAT, SkPyd4p, YLSs alanine aminotransferase, ZmAlaAT1, ZmAlaAT2

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.2 alanine transaminase

Storage Stability

Storage Stability on EC 2.6.1.2 - alanine transaminase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 10% glycerol, half-life of 2 weeks
-
-20°C, 20 mM Tris-HCl, pH 7.5, freezing is worse than storage at 4°C or 25°C
-
-20°C, 50 mM potassium phosphate buffer, pH 7.5, 5 mM 2-mercaptoethanol, 5% glycerol, stable for at least 5 weeks
-
-20°C, stable for several months
-
-25°C, 10 mM potassium phosphate, pH 7.5, 1 mM EDTA, 2 mM 2-mercaptoethanol, 0.04 mM pyridoxal 5'-phosphate, a few days, 50% loss of activity
-
-80°C, 20 mM Tris-HCl, pH 7.5, 20% loss of activity in first two days, thereafter stable for at least 20 days. Addition of 25% glycerol preserves most of the activity
-
-80°C, 20 mM Tris-HCl, pH 7.5, stable for at least 20 days. Addition of 25% glycerol preserves most of the activity
-
0-5°C, 50 mM potassium phosphate buffer, pH 7.5, 5 mM 2-mercaptoethanol, 5% glycerol, stable for 2 weeks
-
0°C, stable for several h in solution
-
25°C, 20 mM Tris-HCl, pH 7.5, 56% loss of activity in first two days. Addition of 25% glycerol preserves most of the activity
-
25°C, 20 mM Tris-HCl, pH 7.5, 80-90% residual activity after 10 days, 60% residual activity after 20 days. Addition of 25% glycerol preserves most of the activity
-
37°C, 20 mM Tris-HCl, pH 7.5, 50% loss of activity in 24 h, and complete loss of activity within one week
-
37°C, 20 mM Tris-HCl, pH 7.5, 72% loss of activity in first two days
-
4°C, 12 days, 57% residual activity
4°C, 20 mM Tris-HCl, pH 7.5, 53% loss of activity in first two days. Addition of 25% glycerol preserves most of the activity
-
4°C, 20 mM Tris-HCl, pH 7.5, stable for at least 20 days. Addition of 25% glycerol preserves most of the activity
-
4°C, 80% saturated solution of ammonium sulfate, 10 days, 40% loss of activity
-