Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Aminooxyacetate | 2 mM, complete inhibition | Candida maltosa | |
Hg2+ | 0.1 mM, 81% inhibition | Candida maltosa | |
hydroxylamine | 2 mM, 73% inhibition | Candida maltosa | |
p-hydroxymercuribenzoate | 1 mM, 87% inhibition | Candida maltosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
pyruvate | pH 7.5, 25°C | Candida maltosa | |
0.37 | - |
2-oxoglutarate | pH 7.5, 25°C | Candida maltosa | |
2.6 | - |
L-alanine | pH 7.5, 25°C | Candida maltosa | |
6.3 | - |
L-glutamate | pH 7.5, 25°C | Candida maltosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
2 * 52000, SDS-PAGE | Candida maltosa |
99000 | - |
gel filtration | Candida maltosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida maltosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Candida maltosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
156 | - |
reverse reaction | Candida maltosa |
594 | - |
forward reaction | Candida maltosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanine + 2-oxobutyrate | 10.2% of the activity with 2-oxoglutarate | Candida maltosa | pyruvate + 2-aminobutanoate | - |
? | |
L-alanine + 2-oxoglutarate | - |
Candida maltosa | pyruvate + L-glutamate | - |
r | |
L-glutamate + 2-oxobutyrate | 32.4% of the activity with pyruvate | Candida maltosa | 2-oxoglutarate + 2-aminobutanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52000, SDS-PAGE | Candida maltosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Candida maltosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | strong decrease above | Candida maltosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
30 min, 50% loss of activity at pH 7.5, 5% loss of activity at pH 9.0 | Candida maltosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | - |
Candida maltosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | approx. 70% of maximal activity at pH 5.5, approx. 50% of maximal activity at pH 9.5 | Candida maltosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
40°C, 30 min, 50% loss of activity | Candida maltosa |
8.5 | 9.5 | more stable at alkaline pH than at neutral pH | Candida maltosa |
9 | - |
40°C, 30 min, 5% loss of activity | Candida maltosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | a pyridoxal phosphate protein, 2 mol pyridoxal phosphate per mol of enzyme | Candida maltosa |