2.4.1.99: sucrose:sucrose fructosyltransferase
This is an abbreviated version!
For detailed information about sucrose:sucrose fructosyltransferase, go to the full flat file.
Word Map on EC 2.4.1.99
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2.4.1.99
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fructans
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fructan:fructan
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fructosyltransferases
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inulin
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1-kestose
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intybus
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invertases
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cichorium
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chicory
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sucrose:fructan
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6g-fructosyltransferase
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inulin-type
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1-exohydrolase
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tuberosus
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6g-fft
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fructooligosaccharides
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nystose
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tequilana
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levansucrase
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cerrado
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exohydrolases
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herbacea
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perenne
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taproot
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scolymus
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cynara
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oligofructans
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3.2.1.80
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beta-fructosidase
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synthesis
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agriculture
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nutrition
- 2.4.1.99
- fructans
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fructan:fructan
- fructosyltransferases
- inulin
- 1-kestose
- intybus
- invertases
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cichorium
- chicory
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sucrose:fructan
- 6g-fructosyltransferase
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inulin-type
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1-exohydrolase
- tuberosus
- 6g-fft
- fructooligosaccharides
- nystose
- tequilana
- levansucrase
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cerrado
-
exohydrolases
- herbacea
- perenne
- taproot
- scolymus
-
cynara
- oligofructans
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3.2.1.80
- beta-fructosidase
- synthesis
- agriculture
- nutrition
Reaction
2 sucrose
=
Synonyms
1-SST, 1-sucrose:sucrose fructosyltransferase, 6-SST, 6G-FFT2, fructooligosaccharide-producing extracellular enzyme, fructosyl-transferase, fructosyltransferase, sucrose 1F-, FST-1, FT1, FTA, SST, Suc:Suc 1-fructosyltransferase, sucrose 1F-fructosyltransferase, sucrose-sucrose 1-fructosyltransferase, sucrose: sucrose 1-fructosyltransferase, sucrose:sucrose 1-D-fructosyltransferase, sucrose:sucrose 1-fructosyltransferase, sucrose:sucrose 1F-beta-D-fructosyltransferase, sucrose:sucrose 6-fructosyltransferase, sucrose:sucrose fructosyltransferase, sucrose:sucrose-1-fructosyltransferase
ECTree
2.4.1.99 sucrose:sucrose fructosyltransferaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.4.1.99 - sucrose:sucrose fructosyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,1-nystose + 1,1-nystose
1,1,1-fructosylnystose + 1-kestose
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self-transfructosylation at a low rate
-
?
1-kestose + 1-kestose
1,1-nystose + sucrose
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self-transfructosylation at a low rate
-
?
1-kestose + 1-kestose
D-fructose + sucrose + ?
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and formation of some nystose
-
?
1-kestose + 1-kestose
fructose + nystose
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23% activity compared to sucrose
-
-
?
2 sucrose
D-glucose + beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside
crystalline cane sugar
1-kestose + sucrose + D-glucose + nystose
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-
-
-
?
fructosyl-nystose + H2O
?
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4% activity compared to sucrose. The reaction generates mainly hydrolytic products
-
-
?
nystose + H2O
?
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8% activity compared to sucrose. The reaction generates mainly hydrolytic products
-
-
?
raffinose + H2O
?
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14% activity compared to sucrose. The reaction generates mainly hydrolytic products
-
-
?
stachyose + H2O
?
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2% activity compared to sucrose. The reaction generates mainly hydrolytic products
-
-
?
sucrose + 6G(1-beta-fructofuranosyl)2-sucrose
D-glucose + 1F-beta-fructofuranosyl-6G(1-beta-fructofuranosyl)2-sucrose
-
-
-
?
sucrose + neokestose
D-glucose + 1F,6G-di-beta-fructofuranosylsucrose
-
-
-
?
sucrose + sucrose
1-kestose + nystose + fructosylnystose
-
-
18.7% (w/w) 1-kestose, 35.6% (w/w) nystose and 6.4% (w/w) fructosylnystose. The rest of carbohydrates in the mixture are fructose (minoritary), glucose and remaining sucrose
-
?
sucrose + sucrose
D-glucose + 6-kestose
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FT1 has high affinity for 6-kestose and low affinity for 1-kestose
-
-
?
1-kestose
nystose + D-fructose
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plus sucrose as a secondary product
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?
1-kestose
nystose + D-fructose
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plus sucrose as a secondary product
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?
2 sucrose
D-glucose + beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside i.e. 1-kestose
-
-
?
2 sucrose
D-glucose + beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside i.e. 1-kestose
-
-
?
2 sucrose
D-glucose + beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside
-
-
-
-
?
sucrose + sucrose
1-kestose + D-glucose
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no production of nystose or fructose from glucose
-
?
sucrose + sucrose
1-kestose + D-glucose
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no production of nystose or fructose from glucose
-
?
sucrose + sucrose
1-kestose + D-glucose
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plus a small amount of fructose and traces of nystose, products of both native enzyme and chimeric enzyme with large subunit of festuca enzyme and the small subunit of barley sucrose:fructan 6-D-fructosyltransferase
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?
sucrose + sucrose
D-glucose + 1-kestose
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the enzyme catalyzes the first step, transferring one fructose moiety between two sucrose molecules
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-
?
sucrose + sucrose
D-glucose + 1-kestose
1-SST uses sucrose as a preferential donor and acceptor substrate, 1-SST can only create a beta(2->1) linkage between two fructosyl residues
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-
?
sucrose + sucrose
D-glucose + 1-kestose
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1-SST activity is about 5times higher than 6-SST activity when the protein is incubated with sucrose for short periods
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-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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-
-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
-
-
-
-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
-
-
-
r
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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the enzyme initiates fructan biosynthesis
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-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
-
-
-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
key enzyme in fructan biosynthesis. After 1 month of growth plantelets do not contain the enzyme. The onset of fructan synthesis coincides with the increase in 1-SST activity in roots. Expression of the 1-SST gene can be observed in roots and leaves of stressed plants
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-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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the enzyme initiates fructan biosynthesis
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-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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EC 2.1.4.99, EC 2.4.1.100 and EC 3.2.1.26 simultaneously control fructan in young chicory roots
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-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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-
1-kestose is the only fructan product
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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the enzyme is the entry point of fructan synthesis, giving rise to the accumulation of 1-kestose and nystose on expression in plant storage organs
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-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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-
-
-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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-
formation of isokestose and upon prolonged incubation some nystose
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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the enzyme is involved in fructan biosynthesis
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-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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the enzyme initiates fructan biosynthesis
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-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
-
-
-
-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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-
production of 1-kestose exclusively
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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the inducible enzyme is the key enzyme diverting sucrose into the biosynthetic pathways of the fructans by formation of 1-kestose, can act in concert with sucrose-sucrose fructosyl-6-transferase to initiate fructan accumulation in barley leaves
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-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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the enzyme initiates fructan biosynthesis
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-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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and moderate production of nystose
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
-
-
-
-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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key enzyme initiating fructan synthesis
-
-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
-
-
-
-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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the enzyme is important in fructan accumulation during cold hardening of winter wheat
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-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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first enzyme in the biosynthetic pathway of most fructans
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-
?
sucrose + sucrose
D-glucose + 1F-beta-D-fructosylsucrose
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the enzyme initiates fructan biosynthesis
-
-
?
additional information
?
-
Agave vera cruz
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free fructose, glucose and raffinose also serves as acceptor for fructose units
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-
?
additional information
?
-
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the pure enzyme displays the preference: sucrose > raffinose > 1-kestose > nystose (in a ratio 100:24:10:6). Other carbohydrates, such as turanose, cellobiose, melibiose, leucrose, methyl-alpha-dglucopyranoside and stachyose are not substrates
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-
?
additional information
?
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in silico evaluation of the interactions between substrate and native fructooligosaccharides-synthesizing enzyme from fungus, docking study and molecular dynamic simulation, comparison with other fungal fructooligosaccharides-synthesizing enzymes, overview
-
-
?
additional information
?
-
-
in silico evaluation of the interactions between substrate and native fructooligosaccharides-synthesizing enzyme from fungus, docking study and molecular dynamic simulation, comparison with other fungal fructooligosaccharides-synthesizing enzymes, overview
-
-
?
additional information
?
-
-
de-novo synthesis of fructans from sucrose in vitro by a combination of two purified enzymes: sucrose:sucrose 1-fructosyl transferase and fructan:fructan 1-fructosyl transferase
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-
?
additional information
?
-
-
in combination EC 2.4.1.99 and EC 2.4.1.100 can synthesize long-chain inulins in vitro from sucrose
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-
?
additional information
?
-
-
the enzyme also catalyzes the removal of the terminal fructosyl unit from both 1-kestose and 1,1-nystose, which results in the release of sucrose and 1-kestose, respectively and free fructose. The enzyme has no hydrolytic activity against sucrose
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-
?
additional information
?
-
enzyme ins the pacemaker enzyme of fructan synthesis in barley leaves
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-
?
additional information
?
-
1-SST shows relatively low hydrolase activity at all offered sucrose concentrations as compared to the glycosyltransferase activity
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-
?
additional information
?
-
-
no activity with melezitose, inulin and levan
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-
-
additional information
?
-
-
the main 1-SST-catalyzed reaction is the formation of 1-kestotriose and glucose from two sucrose molecules. During incubation with 6G-kestotriose and sucrose, the enzyme is again able to form 1-kestotriose from sucrose. 1+6G-kestotetraose is formed during incubation with 6G-kestotriose and sucrose, also cf. EC 2.4.1.243
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-
?
additional information
?
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the main 1-SST-catalyzed reaction is the formation of 1-kestotriose and glucose from two sucrose molecules. During incubation with 6G-kestotriose and sucrose, the enzyme is again able to form 1-kestotriose from sucrose. 1+6G-kestotetraose is formed during incubation with 6G-kestotriose and sucrose, also cf. EC 2.4.1.243
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-
?
