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2.4.1.99: sucrose:sucrose fructosyltransferase

This is an abbreviated version!
For detailed information about sucrose:sucrose fructosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.99

Reaction

2 sucrose =

D-glucose
+
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside

Synonyms

1-SST, 1-sucrose:sucrose fructosyltransferase, 6-SST, 6G-FFT2, fructooligosaccharide-producing extracellular enzyme, fructosyl-transferase, fructosyltransferase, sucrose 1F-, FST-1, FT1, FTA, SST, Suc:Suc 1-fructosyltransferase, sucrose 1F-fructosyltransferase, sucrose-sucrose 1-fructosyltransferase, sucrose: sucrose 1-fructosyltransferase, sucrose:sucrose 1-D-fructosyltransferase, sucrose:sucrose 1-fructosyltransferase, sucrose:sucrose 1F-beta-D-fructosyltransferase, sucrose:sucrose 6-fructosyltransferase, sucrose:sucrose fructosyltransferase, sucrose:sucrose-1-fructosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.99 sucrose:sucrose fructosyltransferase

Temperature Stability

Temperature Stability on EC 2.4.1.99 - sucrose:sucrose fructosyltransferase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
the enzyme is inactivated at 100°C after 10 min
20 - 37
-
10 min, stable
25
optimal stability at
30
-
pH 4.0-8.0, 30 min, stable
30 - 50
-
the half-life values of the enzyme incubated at 30, 40 and 50°C are more than 7 days, 74 h and 16 h, respectively. Temperatures higher than 55°C inactivated the enzyme. In the absence of methyl-alpha-D-glucopyranoside, the enzyme behaves significantly less thermostable (half-life of 42 min at 40°C in 100 mM sodium acetate buffer, pH 5.5)
35
rapid inactivation above
50 - 60
-
10 min, inactivation
55
-
24 h, stable up to
65
-
24 h, about 50% loss of activity
66
-
very stable at temperatures below 66°C, the half-life of the enzyme decreases very quickly at temperatures above 66°C