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Literature summary for 2.4.1.99 extracted from
- Purification and kinetic characterization of a fructosyltransferase from Aspergillus aculeatus (2007), J. Biotechnol., 128, 204-211.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| SDS | 1.5fold activation at 10 mM | Aspergillus aculeatus |  |
| sodium deoxycholate | 1.4fold activation at 1 mM | Aspergillus aculeatus | |
| Triton X-100 | 1.4fold activation at 5% (w/v) | Aspergillus aculeatus | |
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | high transferase/hydrolase ratio confers it a great potential for the industrial production of prebiotic fructooligosaccharides | Aspergillus aculeatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 27 | - |
sucrose | 60°C, pH 5.5, hydrolytic activity | Aspergillus aculeatus | |
| 535 | - |
sucrose | 60°C, pH 5.5, transferase activity | Aspergillus aculeatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
2 * 65000, SDS-PAGE | Aspergillus aculeatus |
| 135000 | - |
- |
Aspergillus aculeatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus aculeatus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | enzyme possesses about 20% (w/w) carbohydrate | Aspergillus aculeatus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus aculeatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2635 | - |
- |
Aspergillus aculeatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the pure enzyme displays the preference: sucrose > raffinose > 1-kestose > nystose (in a ratio 100:24:10:6). Other carbohydrates, such as turanose, cellobiose, melibiose, leucrose, methyl-alpha-dglucopyranoside and stachyose are not substrates | Aspergillus aculeatus | ? | - |
? | |
| sucrose + H2O | - |
Aspergillus aculeatus | D-fructose + D-glucose | - |
? | |
| sucrose + sucrose | - |
Aspergillus aculeatus | 1-kestose + nystose + fructosylnystose | 18.7% (w/w) 1-kestose, 35.6% (w/w) nystose and 6.4% (w/w) fructosylnystose. The rest of carbohydrates in the mixture are fructose (minoritary), glucose and remaining sucrose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 65000, SDS-PAGE | Aspergillus aculeatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Aspergillus aculeatus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 75 | 40°C: about 60% ofmaximal activity, 75°C: about 80% of maximal activity | Aspergillus aculeatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
24 h, stable up to | Aspergillus aculeatus |
| 60 | - |
24 h, about 5% loss of activity | Aspergillus aculeatus |
| 65 | - |
24 h, about 50% loss of activity | Aspergillus aculeatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 775 | - |
sucrose | 60°C, pH 5.5, hydrolytic activity | Aspergillus aculeatus | |
| 16200 | - |
sucrose | 60°C, pH 5.5, transferase activity | Aspergillus aculeatus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | substrate sucrose | Aspergillus aculeatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | pH 4.0: about 50% of maximal activity, pH 8.0: about 79% of maximal activity | Aspergillus aculeatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
24 h, about 65% loss of activity | Aspergillus aculeatus |
| 4 | - |
24 h, about 50% loss of activity | Aspergillus aculeatus |
| 5.5 | 7.5 | 24 h, stable, pH stability is assayed in the presence of high concentration of a carbohydrate (600 g/l alpha-D-methylglucopyranoside) | Aspergillus aculeatus |
| 8 | - |
24 h, about 20% loss of activity | Aspergillus aculeatus |
| 9 | - |
24 h, about 30% loss of activity | Aspergillus aculeatus |
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