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Literature summary for 2.4.1.99 extracted from

  • Lasseur, B.; Schroeven, L.; Lammens, W.; Le Roy, K.; Spangenberg, G.; Manduzio, H.; Vergauwen, R.; Lothier, J.; Prudhomme, M.P.; Van den Ende, W.
    Transforming a fructan:fructan 6G-fructosyltransferase from perennial ryegrass into a sucrose:sucrose 1-fructosyltransferase (2009), Plant Physiol., 149, 327-339.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Lolium perenne

General Stability

General Stability Organism
several attempts to purify 1-SST are unsuccessful, probably due to the instability of the enzyme and/or the rather low concentration in the yeast's expression medium Lolium perenne

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
272
-
sucrose wild type enzyme, in 50 mM sodium acetate buffer, pH 5.0, at 30°C Lolium perenne

Organism

Organism UniProt Comment Textmining
Lolium perenne Q84RM0 perennial ryegrass
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, several attempts (concanavalin A, Mono Q, Mono S) to further purify 1-SST are unsuccessful, probably due to the instability of the enzyme and/or the rather low concentration in the yeast's expression medium Lolium perenne

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + sucrose 1-SST uses sucrose as a preferential donor and acceptor substrate, 1-SST can only create a beta(2->1) linkage between two fructosyl residues Lolium perenne D-glucose + 1-kestose
-
?

Synonyms

Synonyms Comment Organism
1-SST
-
Lolium perenne
sucrose:sucrose 1-fructosyltransferase
-
Lolium perenne