2.4.1.99: sucrose:sucrose fructosyltransferase

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For detailed information about sucrose:sucrose fructosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.99

2.4.1.99

fructans

fructan:fructan

fructosyltransferases

inulin

1-kestose

intybus

invertases

cichorium

chicory

sucrose:fructan

6g-fructosyltransferase

inulin-type

1-exohydrolase

tuberosus

6g-fft

fructooligosaccharides

nystose

tequilana

levansucrase

cerrado

exohydrolases

herbacea

perenne

taproot

scolymus

cynara

oligofructans

3.2.1.80

beta-fructosidase

synthesis

agriculture

nutrition

Reaction

2 sucrose =

beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl alpha-D-glucopyranoside

Synonyms

1-SST, 1-sucrose:sucrose fructosyltransferase, 6-SST, 6G-FFT2, fructooligosaccharide-producing extracellular enzyme, fructosyl-transferase, fructosyltransferase, sucrose 1F-, FST-1, FT1, FTA, SST, Suc:Suc 1-fructosyltransferase, sucrose 1F-fructosyltransferase, sucrose-sucrose 1-fructosyltransferase, sucrose: sucrose 1-fructosyltransferase, sucrose:sucrose 1-D-fructosyltransferase, sucrose:sucrose 1-fructosyltransferase, sucrose:sucrose 1F-beta-D-fructosyltransferase, sucrose:sucrose 6-fructosyltransferase, sucrose:sucrose fructosyltransferase, sucrose:sucrose-1-fructosyltransferase

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.1 Hexosyltransferases

2.4.1.99 sucrose:sucrose fructosyltransferase

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Results	in table
6	Activating Compound
36	AA Sequence
7	Application
1	CAS Registry Number
24	Cloned(Commentary)
1	Crystallization (Commentary)
10	Expression
3	General Information
3	General Stability
77	Inhibitors
1	Ki Value [mM]
14	KM Value [mM]
4	Localization
10	Metals/Ions
23	Molecular Weight [Da]
19	Natural Substrates/ Products (Substrates)
90	Organism
1	Pathway
14	pH Optimum
3	pH Range
8	pH Stability
1	pI Value
6	Posttranslational Modification
10	Protein Variants
16	Purification (Commentary)
1	Reaction
2	Reaction Type
86	Reference
64	Source Tissue
14	Specific Activity [micromol/min/mg]
118	Substrates and Products (Substrate)
18	Subunits
67	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
4	Temperature Range [°C]
14	Temperature Stability [°C]
3	Turnover Number [1/s]

Purification

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Purification on EC 2.4.1.99 - sucrose:sucrose fructosyltransferase

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10 entries

ammonium sulfate precipitation, several attempts (concanavalin A, Mono Q, Mono S) to further purify 1-SST are unsuccessful, probably due to the instability of the enzyme and/or the rather low concentration in the yeast's expression medium

partial

Q sepharose column chromatography

2 entries