Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Lolium arundinaceum |
Protein Variants | Comment | Organism |
---|---|---|
C203N | the mutant has an almost doubled relative hydrolytic activity in comparison to wild type enzyme | Lolium arundinaceum |
L322F | the mutant shows wild type activity | Lolium arundinaceum |
M173L | the mutant shows wild type activity | Lolium arundinaceum |
W362Y | the mutant has an almost doubled relative hydrolytic activity in comparison to wild type enzyme | Lolium arundinaceum |
Y248V | the mutant shows no activity | Lolium arundinaceum |
Y317W | the mutant shows wild type activity | Lolium arundinaceum |
Y408W | the mutant shows low activity | Lolium arundinaceum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.8 | - |
sucrose | wild type enzyme | Lolium arundinaceum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lolium arundinaceum | Q9FSV7 | before known as Festuca arundinacea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | 1-SST shows relatively low hydrolase activity at all offered sucrose concentrations as compared to the glycosyltransferase activity | Lolium arundinaceum | ? | - |
? | |
sucrose + sucrose | - |
Lolium arundinaceum | D-glucose + 1-kestose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-SST | - |
Lolium arundinaceum |
sucrose:sucrose 1-fructosyltransferase | - |
Lolium arundinaceum |