2.4.1.68: glycoprotein 6-alpha-L-fucosyltransferase This is an abbreviated version!go to the full flat file .
Word Map on EC 2.4.1.68 Reaction
GDP-beta-L-fucose N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein] GDP N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->6)]-beta-D-GlcNAc]-L-asparaginyl-[protein]
Synonyms 6FucT, alfC, alpha(1,6)FT, alpha(1,6)fucosyltransferase, alpha-1,6-fucosyltransferase, alpha-6-fucosyltransferase, alpha1,6-fucosyltransferase, alpha1,6-FucT, alpha1,6FucT, alpha1-6,fucosyltransferase, alpha1-6FucT, alpha6FucT, BN194_28780, fucosyltransferase 8, fucosyltransferase, guanosine diphosphofucose-glycoprotein, FUT8, GDP-fucose glycoprotein fucosyltransferase, GDP-fucose-glycoprotein fucosyltransferase, GDP-L-fuc:N-acetyl-beta-D-glucosaminide alpha1-6fucosyltransferase, GDP-L-fucose-glycoprotein fucosyltransferase, GDP-L-fucose:2-acetamido-2-deoxy-beta-D-glucoside 6-alpha-L-fucosyltransferase, GDPfucose glycoprotein fucosyltransferase, glycoprotein fucosyltransferase, guanosine diphosphofucose-glycoprotein fucosyltransferase, NodZ, NodZ fucosyltransferase
ECTree
Cloned
Cloned on EC 2.4.1.68 - glycoprotein 6-alpha-L-fucosyltransferase
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cloned from cDNA library of MKN45 cells, DNA sequence determination and analysis
complete genomic organization for the gene and cloning of two retina splice variants. The presence of the 5'-untranslated exon B diminishes the amount of FUT8 transcript and decreases the alpha6-fucosyltransferase activity by two-thirds, as compared to FUT8 transcripts starting with exon A or C
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expressed in Escherichia coli
expressed in Sf21 insect cells
expressed in Sf9 insect cells as soluble enzyme lacking the transmembrane region
expression in COS-1 cells, DNA sequence determination and analysis
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expression in Sf21 and in COS-1cell
large-scale production system for recombinant human FUT8, in which the enzyme is produced in soluble form by baculovirus-infected insect cells
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mutant enzymes expressed in COS-1 cells
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2.4.1.68 glycoprotein 6-alpha-L-fucosyltransferase
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