Information on EC 2.4.1.68 - glycoprotein 6-alpha-L-fucosyltransferase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.4.1.68
-
RECOMMENDED NAME
GeneOntology No.
glycoprotein 6-alpha-L-fucosyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
GDP-beta-L-fucose + N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl}asparagine = GDP + N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-[alpha-L-fucosyl-(1->6)]-N-acetyl-beta-D-glucosaminyl}asparagine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycosaminoglycan biosynthesis - keratan sulfate
-
-
Metabolic pathways
-
-
N-Glycan biosynthesis
-
-
Various types of N-glycan biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
GDP-beta-L-fucose:glycoprotein (L-fucose to asparagine-linked N-acetylglucosamine of N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-gluco
This enzyme catalyses a reaction similar to that of EC 2.4.1.214, glycoprotein 3-alpha-L-fucosyltransferase, but transfers the L-fucosyl group from GDP-beta-L-fucose to form an alpha1,6-linkage rather than an alpha1,3-linkage.
CAS REGISTRY NUMBER
COMMENTARY hide
9033-08-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene nodZ
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
gene nodZ
SwissProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
IZD-Mb-0503 cells
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
enzyme down-regulation promotes hemoglobin production and erythroid differentiation
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GDP-beta-fucose + (N-acetylglucosaminyl)2-Man3-GlcNAc2-(6-biotinamidohexanoyl)asparagine
GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of (N-acetylglucosaminyl)2-Man3-GlcNAc2-(6-biotinamidohexanoyl)asparagine + GDP
show the reaction diagram
-
-
-
-
?
GDP-beta-fucose + Gal-N-acetylglucosaminyl-Man5-GlcNAc2-(6-biotinamidohexanoyl)asparagine
GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of Gal-N-acetylglucosaminyl-Man5-GlcNAc2-(6-biotinamidohexanoyl)asparagine + GDP
show the reaction diagram
-
low activity
-
-
?
GDP-beta-fucose + Gal2-(N-acetylglucosaminyl)2-Man3-GlcNAc2-(6-biotinamidohexanoyl)asparagine
GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of Gal2-(N-acetylglucosaminyl)2-Man3-GlcNAc2-(6-biotinamidohexanoyl)asparagine + GDP
show the reaction diagram
-
low activity
-
-
?
GDP-beta-fucose + N-acetylglucosaminyl-Man3-GlcNAc2-(6-biotinamidohexanoyl)asparagine
GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of N-acetylglucosaminyl-Man3-GlcNAc2-(6-biotinamidohexanoyl)asparagine + GDP
show the reaction diagram
-
best substrate
-
-
?
GDP-beta-fucose + N-acetylglucosaminyl-Man5-GlcNAc2-(6-biotinamidohexanoyl)asparagine
GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of N-acetylglucosaminyl-Man5-GlcNAc2-(6-biotinamidohexanoyl)asparagine + GDP
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + asialo-agalacto apotransferrin
?
show the reaction diagram
-
-
-
-
?
GDP-beta-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-[alpha-L-fucosyl-(1->6)]-N-acetyl-beta-D-glucosaminyl]asparagine
show the reaction diagram
GDP-beta-L-fucose + reducing terminal GlcNAc of the core structure of asparagine-linked oligosaccharide
?
show the reaction diagram
-
rapid equilibrium random mechanism
-
-
?
GDP-L-fucose + 4-methylumbelliferyl-N,N',N''-triacetyl chitotriose
?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + 4-nitrophenyl penta-N-acetylchitopentaoside
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + 4-nitrophenyl tetra-N-acetylchitotetraose
GDP + ?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + 4-nitrophenyl tri-N-acetylchitotriose
?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + asialo-agalactofetuin
?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + asialo-agalactotransferrin glycopeptide
GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of the asialo-agalactotransferrin glycopeptide + GDP
show the reaction diagram
GDP-L-fucose + factor VII EGF-1 domain
GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of the factor VII EGF-1 domain
show the reaction diagram
GDP-L-fucose + fibulin-1C
?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + glucose regulated glycoprotein of 94 kDa
?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + IgG Fc binding protein
?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + immunoglobulin alpha-1 constant region
?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + immunoglobulin G B12 heavy chain
?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + Man-alpha-(1-6)-[Man-alpha-(1-3)-]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl-asparagine
GDP + Man-alpha-(1-6)-[Man-alpha-(1-3)-]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl-asparagine
show the reaction diagram
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine
GDP + N4-(N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-[alpha-L-fucosyl-(1,6)]-N-acetyl-beta-D-glucosaminyl)asparagine
show the reaction diagram
-
alpha1,6-fucosylation, also referred to as core fucosylation, plays an essential role in various pathophysiological events. FUT8 null mice shows severe growth retardation and emphysema-like lung-destruction as a result of the dysfunction of epidermal growth factor and transforming growth factor-beta receptors
-
-
?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
show the reaction diagram
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine)
show the reaction diagram
-
pH 7.0, 37°C, 6 h, reaction stopped by heating at 100°C for 1 min
-
-
?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine)
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine)
show the reaction diagram
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminy]asparagine
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
show the reaction diagram
GDP-L-fucose + platelet glycoprotein IIIa
?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + polymeric immunoglobulins receptor
?
show the reaction diagram
-
-
-
-
?
GDP-L-fucose + vascular adhesion protein 1
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GDP-beta-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl]asparagine
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-[alpha-L-fucosyl-(1->6)]-N-acetyl-beta-D-glucosaminyl]asparagine
show the reaction diagram
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine
GDP + N4-(N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-[alpha-L-fucosyl-(1,6)]-N-acetyl-beta-D-glucosaminyl)asparagine
show the reaction diagram
-
alpha1,6-fucosylation, also referred to as core fucosylation, plays an essential role in various pathophysiological events. FUT8 null mice shows severe growth retardation and emphysema-like lung-destruction as a result of the dysfunction of epidermal growth factor and transforming growth factor-beta receptors
-
-
?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
show the reaction diagram
Q9AQ17
-
-
-
ir
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminy]asparagine
GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
show the reaction diagram
additional information
?
-
-
one of the enzymes involved in the synthesis of N-linked glycans of the GpIIb/IIIa (CD41a) which is present in megakaryocytes and platelets
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
activation
Ni2+
-
activation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
inactivation
Ca2+
-
-
dGDP
-
-
diethyl dicarbonate
-
10 mM, about 90% inhibition
diphosphate
-
-
EDTA
-
slight inhibition
GDP-glucose
-
-
GDP-mannose
-
-
gefitinib
-
-
iodoacetamide
-
10 mM, about 50% inhibition
iodoacetic acid
-
10 mM, 35% inhibition
Mersalyl
-
5 mM, complete inhibition, inhibition can be very significantly prevented by GDP-fucose, GDP or GnGnGp
methyl iodide
-
1 mM, about 70% inhibition
Mg2+
-
slight inhibition
N-ethylmaleimide
PCMB
-
5 mM, complete inhibition, inhibition can be very significantly prevented by GDP-fucose, GDP or GnGnGp
Phenylglyoxal
-
10 mM, 90% inhibition
pyridoxal 5'-phosphate
-
10 mM, about 85% inhibition
Streptavidin
-
inhibition due to stereochemical blockage of the fucosylation site
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.029 - 0.066
asialo-agalacto-transferrin-glycopeptide
0.0129
Asn-linked agalacto-biantennary sugar chain
-
-
-
0.011 - 0.015
factor VII EGF-1 domain
-
0.0193
GDP-beta-L-fucose
-
-
0.0042 - 0.046
GDP-L-fucose
0.012
N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
pH and temperature not specified in the publication
0.0174 - 0.025
N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine)
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.45
N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
Homo sapiens
-
pH and temperature not specified in the publication
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.9
ADP
-
-
3.1
CDP
-
-
0.0077
dGDP
-
-
4.3
diphosphate
-
-
0.0036
GDP
0.36
GDP-glucose
-
-
0.24
GDP-mannose
-
-
2.3 - 2.8
GMP
0.046
GTP
-
-
2.4
IDP
-
-
5.3
TDP
-
-
4.1
UDP
-
-
1.3
XDP
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00000017
-
fetal liver
0.00000025
-
COS-1 cells
0.00000026
-
adult liver
0.00000036
-
kidney
0.00000037
-
hepatoma cell line AH-66
0.00000072
-
DAB-induced hepatoma cell
0.00000076
-
lung
0.000001
-
stomach
0.0000025
0.0000032
-
spleen
0.0000122
-
brain
0.000035
-
-
0.0000355
-
recombinant enzyme from COS-1 cells
0.000039
-
recombinant enzyme from COS-1 cells
0.000063
-
Sf21-cells
0.000083
-
Sf9-cells
0.000187
-
-
0.417
-
-
0.455
-
purified enzyme
0.497
-
purified enzyme
0.784
-
purified enzyme
4.37
-
purified enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6
-
liver microsomes
6.5
-
spleen
6.7
-
assay at
7.4
-
assay at
8
-
assay at
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 8.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 37
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
14 splice variants of the alpha6-fucosyltransferase gene are expressed early in human embryos
Manually annotated by BRENDA team
-
CD34+ cell, activity of 6FucT increases ahead of, and thereafter concomitantly with, cells expressing the CD41a antigen. When the CD41a+ subpopulation of cells is immunoselected (using anti-CD61 i.e. anti GpIIIa antibodies), its 6FucT activity increases proportionally to the yield of DC61+ cells
Manually annotated by BRENDA team
-
heavy load of 6FucT
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
typical type II membrane protein, which is localized in the Golgi apparatus
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
additional information
PDB
SCOP
CATH
ORGANISM
UNIPROT
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
-
x * 34000 + x * 39000
39000
-
x * 34000 + x * 39000
44000
-
x * 44000, SDS-PAGE
60000
-
x * 60000, SDS-PAGE
62000
-
1 * 62000, SDS-PAGE
66000
2 * 66000, SDS-PAGE
120000
gel filtration
additional information
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
2 * 66000, SDS-PAGE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
no glycoprotein
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in complex with GDP and GDP-fucose, using 350 mM potassium sodium tartrate, 100 mM MES pH 6.5 and 50 mM MgCl2
NodZ protein is crystallized in the presence of phosphate ions in two crystal forms. The enzyme is arranged into two domains of nearly equal size. Although NodZ falls in one broad class (GT-B) with other two-domain glycosyltransferases, the topology of its domains deviates from the canonical Rossmann fold, with particularly high distortions in the N-terminal domain
co-crystallized with GDP-L-fucose
-
hanging drop vapor diffusion method, crystal structure at 2.6 A resolution
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 10
-
-
489335, 489339
4 - 10
4 - 9
-
-
489336
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acetonitrile
Methanol
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C to 37°C: 24 h, no loss of activity
-20°C, no loss of activity, at least 1 month
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
anti-FLAG M2 affinity gel chromatography
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by affinity chromatography with GDP-hexanolamine resin and GnGn-bi-Asn-resin
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from blood platelets by affinity chromatography with an asialo-agalacto-transferrin-glycopeptide resin
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from serum by affinity chromatography with a GDP-hexanolamine resin
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large scale, from brain by affinity chromatography with GDP-hexanolamine resin and GnGn-bi-Asn-resin
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partially from CHO cell culture
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partially from several organs
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloned from cDNA library of MKN45 cells, DNA sequence determination and analysis
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complete genomic organization for the gene and cloning of two retina splice variants. The presence of the 5'-untranslated exon B diminishes the amount of FUT8 transcript and decreases the alpha6-fucosyltransferase activity by two-thirds, as compared to FUT8 transcripts starting with exon A or C
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expressed in Escherichia coli
expressed in Sf21 insect cells
expressed in Sf9 insect cells as soluble enzyme lacking the transmembrane region
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expression in COS-1 cells, DNA sequence determination and analysis
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large-scale production system for recombinant human FUT8, in which the enzyme is produced in soluble form by baculovirus-infected insect cells
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mutant enzymes expressed in COS-1 cells
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme activity is upregulated in colon cancer cells
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enzyme expression is positively regulated by c-Myc and c-Myb
in knockdown mutants only 2 and 3% residual expression levels of FuT8
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C46A
the mutant shows wild type activity
N183/341Q
the mutant shows reduced activity compared to the wild type enzyme
N183D
the mutant shows reduced activity compared to the wild type enzyme
N183D/N341D
the mutant shows reduced activity compared to the wild type enzyme
N183Q
the mutant shows wild type activity
N341D
the mutant shows reduced activity compared to the wild type enzyme
N341Q
the mutant shows reduced activity compared to the wild type enzyme
R359A
inactive
D368A
-
inactive mutant enzyme
D368A/K369A/V370A/G371A/T372A
-
inactive mutant of the flexible loop
D409A
-
inactive mutant enzyme
D410A
-
mutant enzyme is fully active, similar to the wild-type enzyme
D453A
-
inactive mutant enzyme
E373A
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inactive mutant enzyme
K369A
-
inactive mutant enzyme
R365A/R366A
-
inactive mutant of the donor substrate-binding domain
S469A
-
inactive mutant enzyme
Y382A
-
inactive mutant enzyme
D368A/K369A/V370A/G371A/T372A
inactive mutant of the flexible loop
R365A/R366A
inactive mutant of the donor substrate-binding domain
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
Show AA Sequence (223 entries)
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