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Literature summary for 2.4.1.68 extracted from

  • Uozumi, N.; Yanagidani, S.; Miyoshi, E.; Ihara, Y.; Sakuma, T.; Gao, C.X.; Teshima, T.; Fujii, S.; Shiba, T.; Taniguchi, N.
    Purification and cDNA cloning of porcine brain GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1->6fucosyltransferase (1996), J. Biol. Chem., 271, 27810-27817.
    View publication on PubMed
Search for more literature for 2.4.1.68

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS-1 cells, DNA sequence determination and analysis Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibition Sus scrofa
Mn2+ slight inhibition Sus scrofa
additional information no inhibition by EDTA, Mg2+, Ca2+ Sus scrofa
Ni2+ strong inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine)
-
 Sus scrofa
0.046
-
 GDP-L-fucose
-
 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid sequence, alignment and comparison Sus scrofa
58000
-
 x * 58000, SDS-PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
large scale, from brain by affinity chromatography with GDP-hexanolamine resin and GnGn-bi-Asn-resin Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-
brain
-
 Sus scrofa
-
commercial preparation
-
 Rattus norvegicus
-
lung
-
 Rattus norvegicus
-
lung
-
 Sus scrofa
-
spleen
-
 Rattus norvegicus
-
stomach
-
 Rattus norvegicus
-
testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00000036
-
 kidney Rattus norvegicus
0.00000076
-
 lung Rattus norvegicus
0.000001
-
 stomach Rattus norvegicus
0.0000025
-
 testis Rattus norvegicus
0.0000032
-
 spleen Rattus norvegicus
0.0000122
-
 brain Rattus norvegicus
0.000039
-
 recombinant enzyme from COS-1 cells Sus scrofa
4.37
-
 purified enzyme Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine i.e. GnGn-peptide Rattus norvegicus GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine i.e. GnGn-peptide Sus scrofa GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGn-bi-Asn-PABA, synthetic substrate, fluorescent assay Rattus norvegicus GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGnF-bi-Asn-PABA ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGn-bi-Asn-PABA, synthetic substrate, fluorescent assay Sus scrofa GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGnF-bi-Asn-PABA ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) acceptor substrate is derived from bovine gamma-globulin Rattus norvegicus GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGnF-bi-Asn-PABA ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) acceptor substrate is derived from bovine gamma-globulin Sus scrofa GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGnF-bi-Asn-PABA ?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Sus scrofa

Synonyms

Synonyms Comment Organism
alpha1-6FucT
-
 Rattus norvegicus
alpha1-6FucT
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Sus scrofa
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 7.0 Sus scrofa
7
-
-
 Rattus norvegicus
7
-
-
 Sus scrofa