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H125A/D130A
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no enzymatic activity
C122A
about 20-30% of wild-type activity
C313A
about 20-30% of wild-type activity
C334A
located at the extreme C-terminus, about 200% of wild-type activity
C34A
about 20-30% of wild-type activity
DELTAC76
about 20-30% of wild-type activity
additional information
gene silencing of MGAT1 by siRNA
additional information
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gene silencing of MGAT1 by siRNA
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mice lacking the gene Mogat2 , which codes for an MGAT highly expressed in the small intestine, are resistant to obesity and other metabolic disorders induced by high-fat feeding. The Mogat2-deficient mice absorb normal amounts of dietary fat but exhibit a reduced rate of fat absorption, increased energy expenditure, decreased respiratory exchange ratio, and impaired metabolic efficiency. Recombinant expression of the human gene MOGAT2, encoding the enzyme, in the intestine increases intestinal MGAT activity, restores fat absorption rate, partially corrects energy expenditure, and promotes weight gain upon high-fat feeding. The changes in respiratory exchange ratio are not reverted, and the recoveries in metabolic efficiency and weight gain are incomplete
additional information
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mice lacking the gene Mogat2 , which codes for an MGAT highly expressed in the small intestine, are resistant to obesity and other metabolic disorders induced by high-fat feeding. The Mogat2-deficient mice absorb normal amounts of dietary fat but exhibit a reduced rate of fat absorption, increased energy expenditure, decreased respiratory exchange ratio, and impaired metabolic efficiency. Recombinant expression of the human gene MOGAT2, encoding the enzyme, in the intestine increases intestinal MGAT activity, restores fat absorption rate, partially corrects energy expenditure, and promotes weight gain upon high-fat feeding. The changes in respiratory exchange ratio are not reverted, and the recoveries in metabolic efficiency and weight gain are incomplete
additional information
a mutant lacking all five cysteine residues is devoid of monoacylglycerol acyltransferase activity
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a mutant lacking all five cysteine residues is devoid of monoacylglycerol acyltransferase activity
additional information
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MGAT2 knockout mice exhibit a reduction of metabolic efficiency and increased thermogenic energy expenditure when fed a high-fat diet, they are protected from developing obesity, fatty liver, hyperlipidemia, and glucose intolerance despite chronic high-fat feeding
additional information
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knockdown of hepatic lysophosphatidylglycerol acyltransferase 1 gene expression in transfected CHO cells by an adenovirus construct of short hairpin RNA (shRNA)
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Mogat2-targeted 129/SvJae embryonic stem cells are generated with a targeting vector designed to replace exon 1 of Mogat2 including the translation-initiating methionine, Mogat2 (-/-) and wild-type are generated from breeding heterozygotes (first backcrossed with C57BL/6J mice)
additional information
expression of human MGAT2 in the intestine of Mogat2-/- mice promotes the uptake and esterification of monoaclglycerol, and partially restores metabolic efficiency in diet-induced weight gain
additional information
mutant FL-DELTA1-160, with both transmembrane regions deleted, displays relocation in the cytosol. Mutant FL-DELTA161-335, which contains the N-terminus and the two transmembrane domains, exists in the membrane fraction
additional information
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mutant FL-DELTA1-160, with both transmembrane regions deleted, displays relocation in the cytosol. Mutant FL-DELTA161-335, which contains the N-terminus and the two transmembrane domains, exists in the membrane fraction
additional information
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expression of human MGAT2 in the intestine of Mogat2-/- mice promotes the uptake and esterification of monoaclglycerol, and partially restores metabolic efficiency in diet-induced weight gain
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