Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.22 extracted from

  • Yen, C.L.; Cheong, M.L.; Grueter, C.; Zhou, P.; Moriwaki, J.; Wong, J.S.; Hubbard, B.; Marmor, S.; Farese, R.V.
    Deficiency of the intestinal enzyme acyl CoA:monoacylglycerol acyltransferase-2 protects mice from metabolic disorders induced by high-fat feeding (2009), Nat. Med., 15, 442-446.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.22

Application

Application Comment Organism
medicine enzyme deficiency protects mice from metabolic disorders (obesity, glucose intolerance, hypercholesterolemia, and fatty liver) despite high-fat diet, caloric intake is normal and dietary fat is absorbed fully but entry into the circulation is reduced, enzyme may be useful target to treat obesity and other metabolic diseases associated with excessive fat intake Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information Mogat2-targeted 129/SvJae embryonic stem cells are generated with a targeting vector designed to replace exon 1 of Mogat2 including the translation-initiating methionine, Mogat2 (-/-) and wild-type are generated from breeding heterozygotes (first backcrossed with C57BL/6J mice) Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
monoacylglycerol + acyl-CoA Mus musculus
-
 diacylglycerol + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
 129/SvJae and C57BL/6J, treatment starts at an age of 9 weeks
-

Source Tissue

Source Tissue Comment Organism Textmining
enterocyte triacylglycerol synthesis Mus musculus
-
small intestine absorption of dietary fat and storage in white adipose tissue Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
monoacylglycerol + acyl-CoA
-
 Mus musculus diacylglycerol + CoA
-
 ?
monoacylglycerol + acyl-CoA radioactive monoacylglycerol administration to the lumen of the small intestine Mus musculus diacylglycerol + CoA
-
 ?

Synonyms

Synonyms Comment Organism
acyl CoA:monoacylglycerol acyltransferase-2
-
 Mus musculus
Mgat2
-
 Mus musculus

General Information

General Information Comment Organism
malfunction Mogat2 (-/-) mice lack MGAT2 protein and have a greater than 50% decrease in intestinal MGAT activity compared to wild-type mice, they display a normal weight gain and body composition on low-fat diet, with 60% calories from fat knockout mice gain 40% less weight than wild-type mice after 16 weeks, Mogat2 (+/-) mice show an intermediate phenotype, female mice with 60% fat containing diet and males with a 45% fat containing diet also show reduced weight gain, knockout mice show lower fasting insulin concentrations, better glucose tolerance, lower concentrations of total and non-high-density lipoprotein cholesterol in plasma, similar plasma triacylglycerol concentrations as wild-type mice, and less than 5% of wild-type hepatic triacylglycerol content, 7% higher oxygen consumption during active (dark) phase and a higher body temperature (while the mechanism of the increased thermogenisis remains unclear) but similar locomotive activity, similar fat absorption in knockout and wild-type mice, similar fecal fat amounts, fecal mass and energy content, 70% triacylglycerol synthesis in enterocytes compared to wild-type, residual diacylglycerol formation from monoacylglycerol or alternative pathway via breakdown of monoacylglycerol to glycerol and fatty acids and entering into the glycerol-phosphate pathway which is more energy demanding, knockout mice show a reduced rate of fat entering the circulation upon a fat boost, more fat enters the distal intestine, therefore fat entry into the circulation is delayed Mus musculus
metabolism crucial role in assimilation of dietary fat Mus musculus