2.3.1.191: UDP-3-O-(3-hydroxyacyl)glucosamine N-acyltransferase

This is an abbreviated version!
For detailed information about UDP-3-O-(3-hydroxyacyl)glucosamine N-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.191

2.3.1.191

acyltransferases

lps

francisella

trachomatis

chlamydia

lipopolysaccharide

udp-n-acetylglucosamine

homotrimer

drug development

Reaction

a (3R)-3-hydroxyacyl-[acyl-carrier protein]

a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine

a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine

Synonyms

acyl-ACP:UDP-3-O-(3-hydroxyacyl)-GlcN N-acyltransferase, acyltransferase LpxD, CtLpxD, EcLpxD, firA, La0512, LpxD, LpxD1, Lpxd2, PA3646, UDP-3-O-(R-3-hydroxyacyl)-glucosamine acyltransferase, UDP-3-O-acyl-glucosamine N-acyltransferase

ECTree

2 Transferases

2.3 Acyltransferases

2.3.1 Transferring groups other than aminoacyl groups

2.3.1.191 UDP-3-O-(3-hydroxyacyl)glucosamine N-acyltransferase

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Results	in table
1	Activating Compound
23563	AA Sequence
1	Application
14	Cloned(Commentary)
6	Crystallization (Commentary)
22	General Information
2	IC50 Value
11	Inhibitors
11	kcat/KM [mM/s]
7	Ki Value [mM]
25	KM Value [mM]
2	Localization
3	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
33	Organism
11	Pathway
45	PDB ID
2	pH Optimum
16	Protein Variants
7	Purification (Commentary)
1	Reaction
29	Reference
2	Source Tissue
2	Specific Activity [micromol/min/mg]
21	Substrates and Products (Substrate)
4	Subunits
32	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
24	Turnover Number [1/s]

Cloned

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Cloned on EC 2.3.1.191 - UDP-3-O-(3-hydroxyacyl)glucosamine N-acyltransferase

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3 entries

by complementation of a temperature-sensitive Escherichia coli lpxD mutant, a meningococcal chromosomal fragment is cloned that carries the lpxD homologue

Neisseria meningitidis

P95377

698088

expression in Escherichia coli BL21(DE3)

Escherichia coli

Q0P6M7

755699

gene AtlpxD, two AtLpxD isozymes, AtLxpD1 and AtLpxD2, are encoded on chromosome 4, DNA and amino acid sequence determination and analysis, expression as GFP-tagged protein in Arabidopsis thaliana

Arabidopsis thaliana

F4JGP6

720913

gene lpxD, expression as His6-tagged protein in Escherichia coli strain BL21(DE3)

Escherichia coli

718571

gene lpxD, expression of N-terminally His6-tagged LpxD in Escherichia coli strain BL21 (DE3)

Pseudomonas aeruginosa

Q9HXY6

718513

genes lpxD1 and lpxD2, DNA and amino acid sequence determination and analysis, phyylogenetic analysis, the lpxD1gene lies within an essential four-gene operon, lpxD1/fabZ/lpxA/lpxB, while the second gene lpxD2 as a single gene is predicted to be regulated by its own promoter

Francisella tularensis subsp. novicida

720944

lpxA (lpxAPg) and lpxDPg are cloned and expressed in Escherichia coli strains in which the homologous gene is mutated. Lipid A from strains expressing either of the Porphyromonas gingivalis transferases contains 16-carbon hydroxy fatty acids in addition to the normal Escherichia coli 14-carbon hydroxy fatty acids, demonstrating that these acyltransferases display a relaxed acyl chain length specificity

2 entries

LpxD protein modified with an N-terminal His6 tag followed by a one glycine residue linker and the P2A substitution, is constructed and transformed into Escherichia coli Rosetta (DE3)/pLysS

Escherichia coli

P21645

696356

overexpression in Escherichia coli

Escherichia coli

696264

when the wild-type firA gene is cloned into a T7-based expression vector, N-acyltransferase specific activity increases almost 360fold relative to wild-type extracts

Escherichia coli

698693