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Literature summary for 2.3.1.191 extracted from
- A continuous fluorescent enzyme assay for early steps of lipid A biosynthesis (2012), Anal. Biochem., 425, 21-27.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | LpxD is a viable target for antimicrobial development | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene lpxD, expression as His6-tagged protein in Escherichia coli strain BL21(DE3) | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | 0.012 | UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine | pH 8.0, 25°C, recombinant His6-tagged LpxD | Escherichia coli |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3R)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine | Escherichia coli | - |
UDP-2,3-bis[O-(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine + a holo-[acyl-carrier protein] | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene lpxD | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged LpxD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (3R)-3-hydroxymyristoyl-[acyl-carrier protein] + UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine | - |
Escherichia coli | UDP-2,3-bis[O-(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine + a holo-[acyl-carrier protein] | - |
ir | |
| additional information | fluorescent enzyme assay, method optimization, overview | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LpxD | - |
Escherichia coli |
| UDP-3-O-(R-3-hydroxyacyl)-glucosamine acyltransferase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3 | - |
UDP-3-O-[(3R)-3-hydroxymyristoyl]-alpha-D-glucosamine | pH 8.0, 25°C, recombinant His6-tagged LpxD | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | LpxD catalyzes the third step of lipid A biosynthesis, an acyl-acyl carrier protein (ACP)-dependent transfer of a fatty acyl moiety to a UDP-glucosamine core ring, overview | Escherichia coli |
| physiological function | LpxD is essential for survival in Gram-negative bacteria | Escherichia coli |
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Release 2023.1 (January 2023)
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