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2.1.2.10: aminomethyltransferase

This is an abbreviated version!
For detailed information about aminomethyltransferase, go to the full flat file.

Word Map on EC 2.1.2.10

Reaction

[protein]-S8-aminomethyldihydrolipoyllysine
+
tetrahydrofolate
=
[protein]-dihydrolipoyllysine
 +
5,10-methylenetetrahydrofolate
 +
NH3

Synonyms

aminomethyltransferase, Amt, GCVT, glycine decarboxylase complex, glycine synthase, synthase, glycine, T protein, T-protein, T-protein component of glycine cleavage complex, tetrahydrofolate aminomethyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.2 Hydroxymethyl-, formyl- and related transferases
                2.1.2.10 aminomethyltransferase

Crystallization

Crystallization on EC 2.1.2.10 - aminomethyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
aminomethyltransferase/T-protein in complex with dihydrolipoate-bearing H-protein and 5-methyltetrahydrofolate, a complex mimicking the ternary complex in the reverse reaction, purified recombinant wild-type and mutant T-proteins, hanging drop vapor diffusion method, mixing of 22-25 mg/ml proteins, T-protein and H protein, with an equal volume of reservoir solution containing 0.095 M 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid, pH 7.5, 0.19 M calcium chloride, 26.6% v/v PEG 400, and 5% v/v glycerol, at 15°C, 4-5 days to 2 weeks, soaking of the native crystals in mother liquid containing 1 mM (6S)-5-methyltetrahydrofolate at 15°C for 4 h, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement
-
apoform and bound to 5-methyltetrahydrofolate
apoform, tetrahydrofolate complex, folinic acid complex and lipoic acid complex of enzyme, homology model of human enzyme
-