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2.1.1.63: methylated-DNA-[protein]-cysteine S-methyltransferase

This is an abbreviated version!
For detailed information about methylated-DNA-[protein]-cysteine S-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.63

Reaction

DNA (containing 4-O-methylthymine)
+
protein L-cysteine
=
DNA (without 4-O-methylthymine)
 +
protein S-methyl-L-cysteine

Synonyms

Ada protein, Ada-C, AGAT, AGT, AGTendoV, alkylated DNA-protein alkyltransferase, alkylguanine transferase, ATASE, C-hAGT, carboxyl terminal domain of the inducible Escherichia coli ada alkyltransferase, hAGT, methylated-DNA-[protein]-cysteine S-methyltransferase, methylguanine DNA methyltransferase, MGMT, Mgt1, MJ1529, N-hAGT, O6-alkylguanine DNA alkyltransferase, O6-alkylguanine DNA-alkyltransferase, O6-alkylguanine-DNA alkyl-transferase, O6-alkylguanine-DNA alkyltransferase, O6-methylguanine DNA methyltransferase, O6-methylguanine methyltransferase, O6-methylguanine-DNA methyltransferase, O6-methylguanine-DNA-methyltransferase, O6-MGMT, O6meG-DNA alkyltransferase, OGT, Pk-MGMT, SSO2487, Tk-MGMT, TTHA1564

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.63 methylated-DNA-[protein]-cysteine S-methyltransferase

Crystallization

Crystallization on EC 2.1.1.63 - methylated-DNA-[protein]-cysteine S-methyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Cys acceptor site is buried in the protein and, in order for reaction with a DNA substrate to take place, a change in conformation of either the substrate or the protein must occur, Glu-172 and Asn-137 play a major structural role in the AGT protein
-
hanging drop vapor diffusion method, for wild type, enzyme using 0.2 M ammonium acetate, 22% (w/v) PEG 3350, and 0.1 M HEPES pH 7.5, while for mutant enzymes R37K and Y139F using 0.1 M HEPES pH 7.5, 4% polyethylene glycol 8000, and either 4% or 8% ethylene glycol, respectively
sitting drop vapor diffusion method, using 0.1 M HEPES (pH 7.5), and 6% (w/v) polyethylene glycol 8000
sitting drop vapor diffusion method, using 0.35 M potassium nitrate and 1.6 M ammonium sulfate
crystals formed at 20°C by conventional hanging drop-vapor diffusion method
-